Ya Yang 杨涯

@Lagomarsino_L @ibc2024 Decided not to go due to the amount of paperwork and out-of-the-state in-person interview required for the visa application. But Rebekah Mohn will be there presenting our work. Say hi to her!

Finally booked my tickets and am getting excited for @ibc2024 the week after next! Who else will I see in Madrid? #ibc2024 (And of course I am already feeling #evol2024 fomo! Post from Montreal too please!)

@SigmaFacto List the major ones and minor ones separately. I limit my reviews to a single page. Once the authors have addressed the major ones, the paragraphs with the minor issues may have been removed. No need to list them all.

When reviewing a manuscript with many many major issues, do you point out several initial ones or all of them? All of them would take pages but if not all, you may need to do the remaining on the second round for another revision. Ideas? #AcademicTwitter

Still, we found weak signals of a positive association between Mental/Nervous System Disorders and Tyr-enriched metabolisms. Alex comprehensively addressed many caveats of such analyses doi.org/10.1002/ajb2.1…

@saltyecoevo @evoyeyo @Lucia_Lohmann @TribbleTweets @pedrohpezzi doi.org/10.1093/sysbio… by @dfmoralesb

@evoyeyo @yangyayy @Lucia_Lohmann @TribbleTweets @pedrohpezzi

Hey phylogenomic peeps, Do you know manuscripts about Neotropical taxa evolution with phylogenomic datasets available? Please drop them here (thanks a lot in advance!) 👇👇

Where do people do genome skimming with herbarium or partially degraded DNA? We've been using novogen but it does not work well when our samples are partially degraded.

@YinjieQiu A little chilly at night but nice otherwise. We camped at 10,000 feet and it wasn't bad.

@YinjieQiu How’s your metabolomics manuscript?😉

@Lagomarsino_L Yay!!! Sabbatical next?

And it's official- I'm a tenured Associate Professor!!! 🎉🎉🎉

@shantabirdo @CavenderG9 @fkbarker @phylodiversity @mcnulty_lab @gacatail @al_auerbach @mone_maddox @SamScansStuff @thewoodowl It was such a great seminar!! Congratulations!!!

@Lagomarsino_L I've got SSSS so many times that I once got searched four times before getting onto the plane. I even have a student being questions by FBI for 2 hours once before boarding.

A whiplash travel day: my first time selected for extra security screening with the SSSS code, and my first time flying first class (it was cheap, I assume because United is still recovering from recent mishaps). I’d love to repeat one of these, definitely not the other!

Learned how to mount aquatic plants by floating directly onto an herbarium mounting paper today!

A mini Chinese scholar gathering at #Evol2023 😊 @yangyayy @Lin_YiZhang @Xiang__Ji @Siyuan_Feng_ @guan_xiyuan

@ilcneto We usually just run a regular agarose gel before sending for lib prep and sequencing.

@ilcneto Phylogenomics can be done with lower quality RNA. For DGE if you have degraded RNA, after poly-A selection you might get biased sequence coverage. We never done OD 260/280 for our DGE samples either.

#Botany folks, have you had success increasing 260/230 using PEG for RNA extractions with the #RNeasy Plant Mini Kit? Apparently, RNeasy MinElute #Cleanup Kit is not doing a great job for my samples. @QIAGENscience

@ilcneto @QIAGENscience We thrown away some good plant RNA samples earlier on as sequencing cores kept telling us that our RNA were bad. We learned our lessons...

@ilcneto @QIAGENscience I never even measure OD 260/280.

@evoyeyo @pedoconnective @saltyecoevo It was great having you here!

Summer kicked off hecticly amazing! Visited @yangyayy’s lab in UMN, and the NY herbarium thanks to @pedoconnective. The 2-body “thing” is also fun with @saltyecoevo. Now back to the heat of Louisiana. #phdlife

@ilcneto @QIAGENscience Sequencing cores that don't work with plant materials will tell you that you have low RIN number and contaminated RNA, but that is often because they mis-interpret chloroplast rRNA peaks and unfamiliar with plant RNA

@ilcneto @QIAGENscience I never try to clean up RNA extraction. The first step of RNA-seq library prep is two rounds of mRNA enrichment using beeds with poly-T tails. This is a very effective way to clean up RNA.

Biologists from the @yangyayy lab in @umncbs talked about their research on plant pigments and chemistry, while encouraging visitors to make some #SciArt! #SciPride 8/END