Ziv Reich's lab

With a DNA barcoding method called gUMI-BEAR, scientists show how yeast populations adapt to environmental stressors through two distinct modes depending on stress type — rare or prevalent — indicated by their gene expression variability. Read the paper: go.aps.org/40W3OrX

We (finally) managed to publish the first part of my PhD! In many ways, this work challenged most of the fundamental stuff I think about how yeast adapt to stress, the roles and mechanisms of inducing variability, and how we should even approach approximating it.

Here, the authors describe CosMIC as a hybrid approach for large-scale, high-resolution microbial profiling of novel niches. This powerful tool for profiling microbial diversity detected over 40,000 novel small subunits. bit.ly/3RFSw6o #PoweredByPacBio

A new #preprint! Variability is strange, right? Especially when it arises in the background of an identical genetic background. Why would cells, having the same machinery, regulation, and environment, behave differently? Why would there be #noise? 1/13 rb.gy/45y36m

🚨 New preprint alert! 🚨 biorxiv.org/content/10.110… Is microbiome surveying accurate? Meaningful? Robust? For the most part, the answer is sadly underwhelming, especially for those working in undersampled niches. 1/8

🎉New publication alert!🎉 (First yeast one!!🍞🍺) Ever wondered what your life could have been if you'd made different choices? How much of you is determined by your genes? For yeast cells, we have answers! [1/9] journals.plos.org/plosone/articl…

Applying gUMI-BEAR for a large-scale, unsupervised screen of variants containing 2-4 randomly generated mutations in the Hsp82 gene. Using the barcode as a reverse primer, we could isolate variants that made up less than 0.2% of the population for further study. 🧵6/6

Applying the gUMI-BEAR method to track the evolutionary dynamics of 26,000 lineages for 44 days while the population undergoes several temperature changes. 🧵5/6

Happy to share our first microbial evolution preprint describing gUMI-BEAR, a modular, cellular-barcoding method that can be used for large-scale, unsupervised variant screening and to repeatedly track the evolution of the same populations. biorxiv.org/content/10.110… 🧵1/1