Catherine Millar-Haskell
172 posts
Catherine Millar-Haskell
@CatInScience
Biomedical Engineering, PhD * * Extracellular vesicles * * Assay Dev Scientist @ https://t.co/Jh8hMLfsby 🖤🐘Local Goofball 🤍💜
Newark, DE شامل ہوئے Nisan 2019
206 فالونگ180 فالوورز
@CatInScience Sounds interesting! And yes capturing them is tricky, especially in quantities! Good luck with your work
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I love the fact that pipettes were developed by a postdoc tired of doing tedious repetitive tasks.
I wish I could make a fully automated EV isolation system. Or a high throughput sampler for NTA. Or... 🤔
Sketching Science@sketchscience
The micropipette is one of the most essential tools in modern biology, chemistry and medicine. It allows researchers to handle small volumes of liquid with precision and accuracy. But do you know how it was invented?
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I can't believe it's already been a year. :)
ISEV-Journals@ISEV_Journals
Interested in the #TME? Well, these data published in #JExBio in July 2022 demonstrate that the enzyme QSOX1 may have more influence on the tumour microenvironment than generally appreciated. 👉 bit.ly/3ld0u9N #OneYearinJExBio @CatInScience, @lab_slater, @GleghornLab
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@ClarkNano Jalapeño poppers or stuffed peppers are usually a hit! Just need shredded beef, cheese, and peppers :)
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Ideas for shareable appetizers for the cul-de-sac party this Friday? Needs to be easy because I will be working all day, but also impressive because I need to make friends.
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Today while walking through a frosty, swampy area in the woods, I came across this strange looking plant. It smelled like garlic and it was impossible to uproot.
Turns out it's skunk garlic, a thermogenic plant that can generate heat and melt surrounding ice. Pretty dang cool!
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Catherine Millar-Haskell ری ٹویٹ کیا
So glad to see my first 1st author paper is published. I'm very excited to share these findings with the world
AJP-Heart and Circ@ajpheartcirc
#ArticlesInPress: Sex-related external factors influence #pulmonary vascular #angiogenesis in a sex-dependent manner (Hayward-Piatkovskyi, et al.) ow.ly/z9nA50LF2BP @GleghornLab @piatkovskyi @kdocneo @crgonyea @PyleSienna @UDelaware #SexualDimorphism #angiogenesis
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I didn't know powdered sugar came 10x concentrated from the grocery store. Does this mean I have to dilute by a tenth to get a 1x working concentration for my dessert? 🧑🍳
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…And a gentle reminder that negative (null) results are just as meaningful as "positive" ones!
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My second paper from my PhD with @GleghornLab is out as a preprint! A big thanks to my advisor + my lab for supporting me as I tried to figure out what really regulates QSOX1 in pancreatic tumor cells. 🧦My favorite little disulfide bond generating catalyst. 🧦
bioRxiv@biorxivpreprint
Matrix mechanics, not hypoxia, modulate quiescin sulfhydryl oxidase 1 (QSOX1) in pancreatic tumor cells biorxiv.org/cgi/content/sh… #bioRxiv
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@MicrobiomDigest Now, perhaps if they didn't label any of their samples and used different versions of their data (instead of one report), they could end up grabbing the wrong plot by mistake. As someone who has run hundreds, if not thousands of samples, I can't really imagine doing that.
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@MicrobiomDigest From my experience (w/NovoExpress) you can easily duplicate plots and export them. This looks like the same run with the gating shifted slightly to the left. How easy it is to make this mistake by accident: not easy at all. You'd have to have pretty bad with data management...
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For people experienced in #FlowCytometry, how easy would it be to reuse the same plot but put different percentages on them? Is this a mistake that is easy to make?
Caner Geyik@luneris
Still trying to find that "one" early apoptotic cell that made the 0.11% difference in siFosB group. @MicrobiomDigest
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@Joadwe @aquicarattino You're correct, it's NTA. I think NTA is "precise" (at least in its engineering), but it is absolutely not accurate. Event then - if a filter truly removed most EVs > 0.22 um, then I doubt we'd see a peak specifically around 370 nm. But I'm only speculating.
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I have a random question for the #extracellularvesicles community that I've been mulling over for a while: why do people use 0.22 um filters instead of, let's say, 0.45 um filters to clarify conditioned media prior to EV isolation/purification?
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@aquicarattino I wouldn't say they are working poorly, necessarily. Lipid-based particles behave differently than other types of nanoparticles. They can deform and squeeze through the pores more easily. They are also more fragile. But bottle top filters do a good job of removing cell debris.
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@CatInScience Interesting! Is there any speculation on why filters are working so poorly?
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@aquicarattino No, this is not real data. In hindsight, I probably should have put a watermark across the plot to make it more obvious. I made the plot based on data that I've seen in numerous papers as well as my own observations. Here is a real plot showing >30% of the particles are >200 nm
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@CatInScience Is this real data? I wonder how come almost 30% of the particles have a size bigger than the filter pore size. Is this an acceptable ratio for the downstream measurements?
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@fadi_abz That's a good point - the material (PES, PP, PTFE) will certainly change how EVs pass through and using a syringe filter can create a lot of back pressure. I think both EV yield and quality may be adversely affected. Curious if anyone has set up experiments to test this already.
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@CatInScience There is already doubts about 0.22 um filtration due to the possibility of EVs sticking to filters or to larger EVs being disintegrated into smaller vesicles by the force applied during filtration.
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I think performing a filtration step is necessary for non-adherent cell culture, but is it needed for adherent cells? Has the community looked into alternate clarification steps? @IsevComms
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Is there a concern that the size cut-off overlaps with many EV subpopulations? For some studies, would this selection bias alter or skew results?
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I'm in my third month of being jobless after graduating with my PhD. I'm financially broke although my extended family is taking care of me. Is this normal? Everyone is saying the job market is hot right now but I can't find any interested employer. I'm at a loss for what to do.
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