Kyle G Daniels

🚨 New Preprint 🚨 A continuous landscape of signaling encodes a corresponding landscape of CAR T cell phenotype How can we tune JAK/STAT signaling to create better immune cell therapies? doi.org/10.1101/2025.0…

I am so excited to share our new paper in @Nature: the first programmable, site-specific integration of a large DNA payload into T cells in vivo. A single IV injection results in therapeutic levels of TRAC-targeted CAR T cells in multiple models. #Ack1" target="_blank" rel="nofollow noopener">nature.com/articles/s4158… a 🧵

🚨🧬An in vivo cell therapy engineering platform of transformative potential. Congratulations to @j_eyquem and team on a breathtaking (and groundbreaking) body of work. @parkerici @UCSF nature.com/articles/s4158…

Today we report single-cell APEX-seq (scAPEX-seq) — a new method for unbiased mapping of *subcellular* transcriptomes at single-cell resolution. This approach reveals cell states that are not detectable by standard scRNA-seq, and enabled us to identify regulators of CAR T function that improve solid tumor killing. biorxiv.org/cgi/content/sh…

An honor to host the one and only @EricTopol. Of course, we talk about medical futurism. And, of course, we talk about Super Agers (his latest book). But we also talk about his journey from medical student to world’s best known cardiologist to medicine’s foremost soothsayer!

Annual reminder: if you’ve been accepted to multiple graduate programs and are still deciding, please let the ones you’re definitely not going to know as soon as possible! -Someone who got into his PhD off the waitlist the day after the deadline

Excited to take part in @sitcancer Spring Scientific - Next-gen Cell Therapies, T cell Engagers, and Combinations - in Tucson & Virtual on March 25–27. An amazing speaker lineup of luminaries from academia and industry. Registration closes March 25th! sitcancer.org/edu/2026-sprin…

Almost at the end of my PhD, and really excited and grateful to share that I'm joining @StanfordGSB as an Assistant Professor in Fall 2027, following a postdoc at Stanford @SIEPR! 🎉

@TLeavery @LocasaleLab Same here. But electricity, trash, custodial services, hazardous waste disposal are part of research. And govt has contracting standards that require unis be able to do expensive things like dispose of hazardous waste, track dangerous chemicals, and track financial compliance.

@ScienceKyle @LocasaleLab I’d much rather more go to the research

Universities with large academic medical centers are not small charities struggling to keep the lights on. They are part of sprawling financial complexes involving hospital systems, real estate empires, entertainment franchises, venture funds, and multi-billion-dollar endowments that often operate like hedge funds. Annual revenues at many of these institutions can approach or exceed $10 billion. Yet when the research funding model is questioned, the discussion suddenly turns to who will pay for janitors and light bulbs. Meanwhile, new research institutions can be run far more efficiently — potentially with overhead closer to ~15–25% of direct research costs.

Excited to share our new @Nature paper! We developed an IL-2–TGFβ co-agonist that enables efficient induction of antigen-specific pTregs and establishment of immune tolerance in vivo. An important step toward programmable immune tolerance. rdcu.be/e72lN

Today in @Nature we introduce INSTALL, which bypasses mammalian DNA immune sensing to enable non-viral DNA integration with recombinases—a step toward safe, scalable, and mutation-agnostic genome editing. 🧬 🧵(1/13) nature.com/articles/s4158… @BKleinstiver @CGM_MGH @MassGeneralNews @harvardmed

Happy to see this project that started during my PhD come to completion! Beautiful work by @AlexanderRotsch and team solving the structure of the influenza polymerase/elongating RNA polymerase II complex. Congrats to everyone for the amazing story! #author-information" target="_blank" rel="nofollow noopener">nature.com/articles/s4158…

Finally out - fully-defined, feeder-free production of CD4+ helper T cells from induced pluripotent stem cells (iPSCs)! Turns out you need to turn Notch down.... a notch 🥁 And you need to fine-tune stimulation of the T cell receptor ⚖️ cell.com/cell-stem-cell…

Tumor immune evasion is programmable🛡️🧠. Our integrated single-cell CRISPR screening framework maps kinase control of T cell–driven tumor states in Glioblastoma(GBM) 🧬and nominates druggable nodes 💊 (e.g., EPHA2/PDGFRA) that blunt evasive programs and boost T cell killing 🔥.

Our work on using Cas13 to treat Uveal Melanoma has just been published! This work represents the first proof-of-concept Cas13-based RNA therapeutic for uveal melanoma and is a example of translating our tools into a disease-specific application. cell.com/molecular-ther…

Aaaand it’s online ahhhhh!!! 🥳🥳 So excited!! The first glimpse of my postdoc work with @chorye @dukecagt. Here, @stefanmgolas and I developed TurboPRANCE, an open-source robotics platform for rapid and scaled phage-assisted continuous evolutions. 🧪Tweetorial party!👇1/n

I’m pleased to share our lab’s first paper (a review), highlighting new insights into macrophages, their mechanobiology, and cancer therapeutic potential through both genetic and non-genetic approaches. @UCSDJacobs @ucsdbe #Sec2" target="_blank" rel="nofollow noopener">nature.com/articles/s4438…

Study here: science.org/doi/10.1126/sc…

Congratulations to Arc Innovation Investigator Theo Roth (@Roth_Lab) on receiving a 2026 @MichelsonMedRF Next Generation Grant. Theo's team is using combinatorial genetic engineering to create immune cell states not found in nature. michelsonmedicalresearch.org/news/michelson…

@3DiMMUNE Those immune cells are collateral damage here 😅

@ScienceKyle Okay, then it may be reasonable. I have not read the paper since it is behind a paywall. Still, it seems like an unusual target, given that it is fairly common among immune cells.

There is an obvious contradiction in this model: it claims CD70 is largely absent from normal adult tissues, yet develops a more sensitive anti-CD70 CAR T cell to detect levels below conventional detection limits. #science #immunology science.org/doi/10.1126/sc…