Lander Lab

Last chance to apply to the Cold Spring Harbor #cryoem course this March (application deadline is Jan 15th)! Learn all about sample prep, microscope operation, and image processing at this fun 2-week course @kollmanlab @Jen_Cash_11 @cshlcourses meetings.cshl.edu/courses.aspx?c…

Congrats to our grad students who pitched therapeutic targets at this year's @ScrippsGradPrgm Drug Discovery Shark Tank - particularly Shelby @ShelbyFerrier and Morgan @m_m_gee on their winning proposal to target Cholera toxin subunit B, which raised $4 million!

Proud of new work tinyurl.com/yhz4c6ry from the lab by Zhicheng (Chen) Cui @Chen3dem using single-particle cryo-EM on liposomes to show how mTORC1 integrates nutrient and growth factor signaling at lysosomes, collab. w/ @G__Napolitano, A. Esposito, and A. Ballabio.

You can now follow us at bsky.app/profile/lander…

The human mitochondrial ClpXP is uniquely assembled and regulated compared to its bacterial counterparts. Congrats to Kelly Chen on her years of hard work on this challenging complex! biorxiv.org/content/10.110…

@AlanRoseman3 @jhschaef Mystery solved. And fascinating! Thanks Alan

@jhschaef It's collagen VI

Let us know if you recognize this complex

@mimi1inh @scrippsresearch My bench skills are long gone…

@LanderLab @scrippsresearch Gabe, where's yours??? 🤓

Proof our lab skills are cutting-edge—both at the microscope and the (pumpkin carving) bench! 🎃🔬 #CryoEM #PumpkinScience @scrippsresearch

Ready for the final day of the BPS meeting on 'Emerging Theoretical Approaches to Complement Single-Particle Cryo-EM' in Trieste! 🔬 We had outstanding speakers, great science, and friendly atmosphere. Thanks to @BiophysicalSoc @cecamEvents, @palermo_lab @RommieAmaro @LanderLab!

Apply now and come work at @VanderbiltU with me and my outstanding colleagues in the Center for Structural Biology! Would be thrilled to recruit more #NMR friendly colleagues! 😉🙌 @VanderbiltCSB @VandyBiochem @VUBasicSciences

Mesoscale Explorer molstar.org/me/ Paper out !! Excellent work with @asrmoin @dsgoodsell  and @sehnaldavid doi.org/10.1002/pro.51…

Some thoughts on the current state of single particle cryo-EM map/model validation. (TLDR: preferred orientation remains a big problem, ❤️ Q-scores. There's been progress, but there's still more to do. ) authors.elsevier.com/sd/article/S09…

The registration deadline of the meeting "Emerging theoretical approaches to complement single-particle cryo-electron microscopy" is today! Join us in Trieste, Italy, with an outstanding list of speakers. biophysics.org/2024trieste#/ Pls RT & Apply !

@RubinsteinJohn @GonenShane @jzhaosbp Grid factory set up in La Jolla thanks to @RubinsteinJohn & @jzhaosbp! Efforts spearheaded by @lalexandrescu1, carbon or gold with a bunch of different hole sizes and spacing. Come by any time @GonenShane !

It's once again time to order grids. This time for our slated 2027 projects. 🤞we can get some by then

We always thought nucleotide state strictly defined AAA+ conformation, but Jeff Mindrebo's work on mito LONP1 suggests more is at play - substrate interactions also drive protein translocation! Revisiting & rethinking the hand-over-hand mechanism: tinyurl.com/49y7a6h6

@CrollTristan Finally getting around to using this, very pleased with the implementation. Thanks Tristan! Is it possible to color the residues according to Q-score like in the OG Chimera plugin?

Now installable via ChimeraX (Tools/More Tools... and look for QScore) - code is at github.com/tristanic/chim… (MIT license). I was able to make a few optimisations - this version runs at about 0.5-1ms/atom (~10s for "average" cryoEM models). Hope you all find it useful! (2/2)

Given ISOLDE is a model-fitting tool, it was about time I gave it a way to, you know, actually validate the fit of a model to a map. As such, in consultation with Greg Pintille and Tom Goddard I've implemented the Q-Score algorithm (nature.com/articles/s4159…) in ChimeraX. (1/2)

Hey all new(ish) structural biology PIs out there - I started a slack workspace geared toward us! If you’d like to join, DM me your email and method(s) of choice, and I’ll add you to the group.