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So far the morphology of live nematodes appears foggy under a microscope compared to fixed and cleared ones. I have seen a few live nematodes that do come out clear. But none of the recent ones I have mounted have clarity.
@LPerepolkin It's always good to make sure, so that you are certain that when you send of the amplicon for sequencing you'll be getting back nematode DNA. Nothing worse than paying for barcode sequencing and getting fungal sequence data back!! Good luck, keen to see what you find! 2/2
@LPerepolkin Nicely done Lawrence! May I suggest including a negative control in your PCRs? This will confirm that none of your reagents are contaminated. Depending on what gene region you are targeting for amplification, the primers you use can sometimes amplify non-target DNA 1/2
Using the nematode smash technique from Powers' paper and an accommodating Bento Lab dipstick DNA extraction kit protocol gives me a warm and fuzzy feeling when extracting nematode DNA. I see a gel band for all eight nematodes!!!
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We may find M.enterolobii on the Mi tomato variety (resistant to M. incognita, M. javanica) or breaking resistant population of RKN. We'll figure when doing RKN species identification.
@UFGulfCoastNema
Sorghum is an excellent host for root-knot #nematode, Meloidogyne incognita. Inoculated with 16,500 juveniles in a greenhouse test, the population density soared to over 2 million upon test termination 😱
@activeplantnema
The situation in Cairns and Far North Queensland remains critical. A huge thanks to all our emergency services and ADF personnel who are on the ground helping.
Had a great time presenting my work on root-knot nematode diagnostics at the #2023APPSAdelaide conference. A lot of really thought provoking presentations and posters at the conference.
@LPerepolkin Are you finding you can discern more features when they're stained? I used to stain trematodes for morphological characterisation, but thus far have found nematodes to be easy enough to study without stain