David Klebl 리트윗함
These ‘movies’ of proteins in action are revealing the hidden biology of cells nature.com/articles/d4158…
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David Klebl
35 posts
David Klebl
@DavidKlebl
Postdoc @BriggsGroup @MPI_Biochem | PhD at @AstburyCentre @UniversityLeeds | Cryo-EM and cryo-ET
Munich 가입일 Nisan 2018
302 팔로잉233 팔로워
David Klebl 리트윗함
Over 4 years in the making, I am excited to share our pre-print on the “In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-electron tomography”. We used cryo-ET to resolve the copia capsid structure to subnanometer resolution inside cells. A 🧵
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@DavidKlebl @stemuench @DrScarff Many congratulations David, and all other authors!🥳🎂
Great start to 2024 :)
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Really happy to finally share this work! Started some time ago by Howard White, I worked on this during my PhD with @stemuench and now completed by @DrScarff's group 🙂
biorxiv.org/content/10.110…
Charlie Scarff@DrScarff
Interested in how myosin generates movement? and how actin activates it? Check out our preprint: biorxiv.org/content/10.110… @UoL_LICAMM @AstburyCentre Beautiful collaborative effort!!
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David Klebl 리트윗함
The paper is now online in its revised form @naturemethods: nature.com/articles/s4159… . Many thanks to the anonymous reviewers and @rita_strack for the nice review experience. And shoutout to twitterless Oda, JP, and @CJOKaiser! It has been a fun journey. 🥳🥳🥳🥳🎉
Sven Klumpe@svenklumpe
Glad to finally share our manuscript on “Serial Lift-Out” by the amazing twitterless Oda Schiøtz, Jürgen Plitzko, @CJOKaiser , and myself. Doing multiple sections from single lift outs showing beautiful C. elegans ultrastructure 🤯 I could watch it all day 🤩How did we get there?
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Really enjoyed visiting Leeds for the #AstburyRetreat, and it was so nice to see everyone again @AstburyCentre!
Paul Taylor@PaulfrYorkshire
@DavidKlebl on fast time-resolved Cryo EM a fantastic talk including great science, falling cats 🐈⬛ 😆 and photos from Iceland @AstburyCentre #AstburyRetreat
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@BolandLab_GE Thanks @BolandLab_GE! Hopefully we can share some more details on the methods soon
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David Klebl 리트윗함
We're hiring! This new role will be involved in all aspects of facility operation with a focus on working with users to advance structural MS and proteomics projects on our new Orbitrap Eclipse and established UHMR platforms. Find out more and apply at: jobs.leeds.ac.uk/vacancy.aspx?r…
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I'm glad we could make a small contribution to this work: onlinelibrary.wiley.com/doi/10.1111/jm… Very different from our usual samples... ⏱️-❄️🔬 of 💎 @stemuench
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David Klebl 리트윗함
Check out our recent paper on direct extraction from the membrane with novel amphipols! With pandemic interruptions this has felt a long time coming, so happy to see it out 😁
Communications Biology@CommsBio
In this study, the authors present a cycloalkane-modified amphiphilic polymer that can extract membrane proteins for Cryo-EM analysis. @stemuench @AJHiggins12 @FrankSobott @alexjflynn @DrJonLippiat @postis_v nature.com/articles/s4200…
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Our new paper is online 🥳 We show how to prepare EM grids for a broad range of timepoints, to achieve time-resolved cryo-EM. @stemuench @FrankSobott
Structural Biology@ActaCrystD
David P. Klebl et al.: On-grid and in-flow mixing for time-resolved cryo-EM #ElectronMicroscopy #TimeResolved #Dynamics @universityleeds @universityleeds ... #IUCr scripts.iucr.org/cgi-bin/paper?…
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David Klebl 리트윗함
How do middle coat adaptor proteins assemble and contribute to remodel the plasma membrane? Take a look at our manuscript in Nature Comms.
➡️ nature.com/articles/s4146…
#endocytosis
@EMBLHamburg @FrankSobott @svergungroup @DavidKlebl @stemuench @SkruznyLab @CssbHamburg
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David Klebl 리트윗함
Our publication on the invisible hands of sample prep is now visible to everyone! Please check it out to demystify some of your challenges on:
nature.com/articles/s4159…
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@RSBon_Lab @FrankSobott @stemuench @MatthewFeasey @HeskethEmma @naranson That's right. This took a lot longer than we originally thought.. But I'm very happy with the result!
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@FrankSobott @stemuench @DavidKlebl @MatthewFeasey @HeskethEmma @naranson Do I remember correctly that the interesting MS data first appeared in rotation project 2?
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David Klebl 리트윗함
Took a while to get around the preferred orientation of HSPD1 but good to see the hard work of @DavidKlebl and the team @MatthewFeasey @HeskethEmma @RSBon_Lab @naranson @FrankSobott finally out. cell.com/iscience/fullt…
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and here's a link to the final version, published a while ago @Structure_CP: cell.com/structure/full…
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Excited to see our paper on proteins at the air-water interface (almost) published! biorxiv.org/content/10.110…
GIF
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David Klebl 리트윗함
Excited about our review on flanking regions in amyloids! Published today in Frontiers in Neuroscience:
frontiersin.org/articles/10.33…
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@soph_hesk @biorxivpreprint @Javi_Lizaendo @embl @SkruznyLab @svergungroup @stemuench @FrankSobott Thanks Sophie!
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@DavidKlebl @biorxivpreprint @Javi_Lizaendo @embl @SkruznyLab @svergungroup @stemuench @FrankSobott Ohhh this is exciting! Congratulations!!
Am excited to pick your brain about these things at some point haha! ☺️
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Our work on endocytosis adaptor proteins is now on @biorxivpreprint 🥳 Great collaboration with @Javi_Lizaendo, @embl, @SkruznyLab and @svergungroup. And with @stemuench and @FrankSobott, of course!
biorxiv.org/content/10.110…
GIF
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