Alex Shaw

Training for rapid DDNS sequencing of poliovirus at the Institut Pasteur de Madagascar with trainees from Côte d'Ivoire, CAR, Madagascar and Algeria @VaccineEpi @NIBSC_MHRA @MRC_Outbreak @polionanopore

Dr Nuno Faria opening at CADDE Workshop on Portable Metagenomics for Pathogen Surveillance 2023 @CaddeProject @MRC_Outbreak

And to finish off, the lab have performed near-whole genome sequencing of poliovirus in single reads using native barcoding. Great work! @OmaniMOH @VaccineEpi @NIBSC_MHRA @MRC_Outbreak @polionanopore

Rapid analysis of DDNS poliovirus data using @AineToole 's Poliovirus Investigation Resource Automating Nanopore Haplotype Analysis (PIRANHA) software running in ARTIFICE- from RNA to sequences in 2.5 days. @OmaniMOH @VaccineEpi @NIBSC_MHRA @MRC_Outbreak @polionanopore

Great progress on the first two DDNS sequencing runs with the Omani CHPL #polio team! @OmaniMOH @VaccineEpi @NIBSC_MHRA @MRC_Outbreak @polionanopore

Training in direct detection and nanopore sequencing (DDNS) for rapid #polio outbreak detection underway with the Central Public Health Laboratories in Oman. @OmaniMOH @VaccineEpi @NIBSC_MHRA @MRC_Outreak @polionanopore

It’s world #polio day and a wonderful opportunity to share our work on faster poliovirus detection with ⁦@BillGates⁩ ⁦@trevormundel⁩ and Chris Karp Congrats ⁦@AlexShaw_AS⁩ ⁦@PlacideMbala⁩ ⁦@labgenpath⁩ and the polio sequencing consortium

Just arrived for #GrandChallenges 2022! Will be presenting poliovirus and NPEV collaborative surveillance work between @VaccineEpi @labgenpath #INRB @NIBSC_MHRA @AineToole and Andrew Rambaut. @MRC_Outbreak

Congratulations to #INRB @labgenpath team who stands as a key asset for @AfricaCDC Pathogen Genomics Initiative, expanding sequencing capacity to better monitor and control infectious disease #outbreaks in #DRC and beyond in the Central African region. @Central_RCC

Filming now in session for our Direct Detection of poliovirus by Nanopore Sequencing (DDNS) protocol training videos @VaccineEpi @MRC_Outbreak @gatesfoundation with @AlistairMB

postdoc epidemiology / evolutionary biology role with our team working on #polio eradication. opportunity to be involved in the Endgame! Deadline 23 Feb. DM or email me if interested. @ImperialSPH @MRC_Outbreak @gatesfoundation imperial.ac.uk/jobs/descripti…

Our group has an opening for a Research Associate focused on the evolution and epidemiology of poliovirus: imperial.ac.uk/jobs/descripti…

First trial of our remote training for direct detection of poliovirus from stool by nanopore sequencing today, with the Institut National de Recherche Biomédicale in DRC @nanopore @inrb_kinshasa

@firefoxx66 @AineToole @VaccineEpi @NetworkArtic @ION_BRU @TromanCatherine We'd very much like to but it's largely dependent on the local polio team. Ideally we'd get the sequences public, possibly a @nextstrain repository.

@AineToole @VaccineEpi @NetworkArtic @ION_BRU @AlexShaw_AS @TromanCatherine Will the sequences be publicly available afterwards? The lockdown on polio sequence data is rather insane in my experience... It would be great it out to more scientists so we can work on it. (Disclosure, I have a big interest in Enteroviruses!)

En route to Pakistan today with the @VaccineEpi lab! We're bringing the MinION to Islamabad to sequence poliovirus and will be running #RAMPART to analyse the data in real-time. Nerves gone and I'm excited to get there! @NetworkArtic @ION_BRU @AlexShaw_AS @TromanCatherine

Polio sequencing trip episode 3: This time it'll work flawlessly. Off to Pakistan we go.

@MikeyJ @IMIBirmingham @WvSchaik @JessicaMABlair @DrMicroMandroid @samstudio8 @alanmcn1 @pathogenomenick Congratulations Mike! We'll be sorry to loose you! I'm pretty certain @pathogenomenick is just a twitterbot though..

I have some exciting news: I am going to be moving to Birmingham, starting as a Lecturer @IMIBirmingham with lovely folks such as @WvSchaik @JessicaMABlair @DrMicroMandroid @samstudio8 @alanmcn1 and even @pathogenomenick who are real people after all & not just people on twitter.

Anyone inferring more sequencing issues from my lack of tweets would be correct. Two weeks of troubleshooting, results due in the <distant> future. Real science is hard.. if this were a movie we would have obliterated several invading alien species with that amount of effort.

One near heart attack later, after it looked like another failed protocol, we might actually have some polio genomes to sequence. Hopefully I won't succumb to library-prep related trauma first.

Better do one of those selfies (as my work experience kids spent the entire week doing). Spent mins trying to get both the suitcases in and make my face less round. Achieved neither. Prob need an emoji: 🐠

We'll begin with the valiant effort to stuff everything into a suitcase again. Definitely going to work this time. I should probably add a hashtag too.. er.. #Suitcase (I think that's how this works- I'm sure @alis_njc will correct me otherwise)