Alison Fanton
11 posts
@AlisonFanton
Scientist in the Hsu Lab, Arc Institute
A study published today in @NatureBiotech from the @pdhsu lab presents a new way to insert large DNA sequences into the genome using engineered recombinases that don’t require DNA cutting or rely on the cell's repair machinery.
Super proud of the fearless @AlisonFanton for her PhD defense yesterday! Alison is a super talented bioengineer and molecular biologist who has done pioneering work on DNA recombinases for human genome insertion. Congratulations, Dr. Fanton!
🧵In new work, we report a systematic engineering roadmap to optimize large serine recombinases (LSRs) for direct, site-specific insertion into the human genome 🧬. We achieved over 50% insertion efficiency and 97% genome-wide specificity, a 10X improvement over our previous work
.@sitaritapita and I are excited to share RESPLICE, a new RNA-programmable mode of editing that leverages RNA targeting CRISPR proteins and the endogenous spliceosomal machinery to enable versatile RNA edits in human cells! (1/12) biorxiv.org/content/10.110…
Just shared at @KeystoneSymp a new @ArcInstitute discovery of the bridge RNA recombinase mechanism: a new class of natural RNA-guided systems that retains the key property of programmability from RNAi and CRISPR while enabling large-scale genome design beyond RNA and DNA cuts
