RBSCDB

Latin American Developmental Biology Conference #LASDB2024 Valparaíso, Chile, 17-20 March 2024. Abstract deadline: 30 November 2023 meetings.embo.org/event/24-dev-b… #EMBOdevBio

How do we go about generating neural cell types in vitro from human pluripotent stem cells? Generally, we leverage relevant morphogen pathways and iteratively modulate them by trial and error to generate a cell population of interest. This is tedious and we yet have to learn how to derive many disease-relevant cell types. In an effort spearheaded by the amazing @neal_amin & @kevinwkelley we took a first-level, systematic approach to address this question by building an arrayed screen of morphogen in organoids combined with multiplexed single cell transcriptomics. We discovered that this platform can generate considerable regional and cell type diversity across the human neural axis and it provided insights into the specification of interesting neuronal populations of the forebrain or cerebellum. See the results in the preprint below 👇 biorxiv.org/cgi/content/sh…