Daniel Bondeson

Did you know too much phosphate can be toxic to cancer? Super excited to see our paper describing phosphate dysregulation as an anti-cancer strategy, out now in @naturecancer! nature.com/articles/s4301…

We have a postdoc position available for a very cool collaborative project! Synthetic chemistry expertise is a must! Unfortunately due to the funding source, this position is only available to US citizens or permanent residents. Please share and reach out if interested!

Amazing new method for identifying substrates (both endo- and drug-induced-neo) of E3 ligases from @hai_tsang_huang! Congrats!

Happy to post this study on a new method for tryptophan labeling and its use to identify cation-pi interactions led by Xiao @Shaw_XXie and Patrick with @Toste_Group @UCB_Chemistry @berkeleyMCB in @Nature ! nature.com/articles/s4158… 1/n

The goal of teaching should not be to help the students learn how to memorize and spit out information under academic pressure. 🧠 The purpose of teaching is to inspire the desire for learning in them and make them able to think, understand, and question.

@GMBurslem @NRaniszewski1 Congratulations!!

First paper of the year accepted! @NRaniszewski1’s first (of many), first author paper! #proudpi

We have reopened the call for 1 of 2 postdocs in the Nwosu Lab. New deadline is Jan 31. Please kindly RT. In the spirit of the new year, those who applied in 2023 and believe their situation have changed should feel free to send me their updates directly. academicjobsonline.org/ajo/jobs/26453

Looking for a #Postdoctoral Researcher to join the newly established Tsvetkov lab at the @BIDMC_CancerCtr , @BIDMCpath to engage in cutting-edge science exploring questions at the intersection of metabolism, metal and cancer biology. Please reach out if interested and retweet 🙏

@wgibson @J_A_C_S Congratulations!

Our work describing targeted protein relocalization and transcriptional rewiring is now out in @J_A_C_S ! pubs.acs.org/doi/10.1021/ja…

@liangc_science Congrats!

Honored to receive the AACR Scholar-in-Training Award to give a oral presentation at AACR Annual Meeting 2023! I’ll be presenting (most of) my PhD work “Systematic profiling of conditional pathway activation as a new class of cancer dependency”. Let’s meet up in Orlando in April!

Our Pre-print is out, showing that FDX1 is an essential upstream regulator of protein lipoylation in human cells. This is mediated through direct binding of FDX1 to the lipoylating enzyme LIAS and not through Fe-S cluster regulation. @BroadCancer biorxiv.org/cgi/content/sh…

Check out our preprint on design and application of Transcriptional/Epigenetic Chemical Inducers of Proximity (TCIPs), to rewire cancer drivers and activate apoptosis. doi.org/10.1101/2022.1… The concept was to hijack a cancer driver, and reroute it to activate cell death.

Balance is needed when discussing academic careers *both the good and the bad* nature.com/articles/d4158…

@iamgkadam @alessiociulli I wouldn't infer that from these results. These are recombinant degrons, and we don't know whether existing lysines or other features within the POI are contributing to success or failure. That said, there are definitely a lot of factors for what makes a good glue target!

@DanB0nD1 @alessiociulli Great work! 👏👏 Just wondering - is it fair to say baed on these results that also when picking targets for molecular glue approach to a known E3 ligase, need to consider more factors than only the presence of degron in the endogenous aa sequence?

Interested in conditional degron tags for your protein of interest? We are too! But after finding the discovery process slow and inefficient, we developed a new system to more robustly identify degron tags. 🧵 nature.com/articles/s4146…

@alessiociulli @broadinstitute Thank you Alessio! We're now expanding the panel to include BromoTag and other new techs

A first survey comparison of performance of 5 degron systems across 16 targets, both N and C tagged, stably-induced in HEK293 cells. Nice work @DanB0nD1 and team @broadinstitute

@danetteldaniels @lukemsimpson Thank you Danette!

@DanB0nD1 @lukemsimpson Nice work!

@AlexBuhimschi Regardless, I am skeptical that a predictive model could be built that would be good enough to obviate testing multiple strategies. I would instead favor understanding the failures of the existing technologies and then designing a new system without those failure modes.

@AlexBuhimschi Thanks Alex! There are certainly some rules that you can easily predict, like localization or one terminus of your protein embedded in the middle of a protein complex. But I agree that understanding failure modes might help define additional rules.

@DocDellaire They can be very powerful tools! I'm happy to chat through things if you're interested

Have always wanted to try degron tags - here is a nice paper/tweetorial on comparing systems for conditional degradation of proteins. 👇🔥👇🔥

We now welcome proteolysis-targeting antibodies (PROTABs) as degraders of cell surface receptors. @Nature nature.com/articles/s4158…

@jcoker10 @Biologenius @TheSellersLab @TothovaLab @DrFranVazquez @AleIanari Thanks much! That's exactly what we're going for! Cheers

@DanB0nD1 @Biologenius @TheSellersLab @TothovaLab @DrFranVazquez @AleIanari This is awesome—just the kind of rigorous, careful, and AddGene deposited work that makes everyone’s life easier! Congrats 🔥