Doug Miller 🔬⚡

After waiting over 24 hours for @Uber support to respond, I still am unable to use a corporate card with their service. Their support is a complete joke. There is no phone number and no one responds to chats.

In @eLife: Against cortical reorganisation doi.org/10.7554/eLife.… Tamar Makin @plasticity_lab & I argue that one area of the brain cannot take over for another as claimed by remapping studies. Instead brain largely fixed in its capacities but some latent & can be up-regulated.

Excited to share my #MiFoBio2023 PPT on live cell image analysis! Just created a Git with the explanations for Cellpose and TrackMate. Perfect for biologists without any coding experience. Thanks again to @jytinevez lab and @computingnature lab. #deeplearning #Microscopy

@DrNancyPadilla @TrackingActions I'm guessing you would?

Hi there, does anyone have example successful COSYNE abstracts they are willing to share with me and my students? #babyPI

@HashemiLab 0% of the time, it works every time. I am a bastion of cringe humor and I will never change!

@AshleyIngiosi Readily available? Unlikely, as their commercial potential is much less appealing. But, if you're comfortable cloning, it's fairly straightforward creating custom ones to be packaged.

Are there AAVs that express calcium sensors in neuronal subtypes (e.g., GABAergic) without using Cre? For example, AAV-Vgat-GCaMP. I feel like they should exist, but I can't find them.🤔

@LecoqJerome I suspect this meme would serve how all my ideas about how the brain works end up (or rather how it doesn't quite work).

OpenScope 2023 Request for Proposal is open!

Someone I know met a woman who's a Nobel-prize winning biologist and she said "I see you are wearing make-up, that already tells me that you're not focussed and committed enough to make it in science".

@EmbraceTheCoda Oh, I don't miss that at all

@DougMillerNeuro That's the most frustrating. Spending weeks on something you could absolutely do in a day

@AdamB0wman @LecoqJerome Is it possible to do this approach using multiphoton excitation? I'd imagine an slm could be used to acquire images like a widefield, but I'm not well-versed enough with the approach of it would work.

@LecoqJerome Definitely! I'm working on another system with 17 mm crystal aperture for large FOV. This is probably close to the practical limit for transverse field geometry though

Looks like voltage imaging got about another factor 2 improvement, even while discarding most collected photons through a polarizer. Smart. science.org/doi/full/10.11…

Thrilled to finally share my PhD work, out today as a preprint! 🎉 Q: How do the brain’s dopamine and serotonin systems work together to drive learning? 🧠 Short answer, 🧵 below Long answer: 1/9

@EPhisCowboy Definitely get the quartz dialed in, otherwise a P2000 isn't worth it.

NMDAR 2B single channel. This Sutter P2000 pulls really nice pipettes. Still dialing in quartz. This was borosilicate with sylgard. But the tips appear smoother, and I can form seals easier with this puller

What is #resilience and how can we measure it? Our new paper in @EjamJournal shows the answer is not straightforward. We review existing approaches, formalize indicators of attractor resilience in #dynamicalsystems, and develop a new classification. 1/3 bit.ly/3P2PfNu

Check out our Review in @TrendsCellBio! "How it feels in a cell" @bonucci_martina and Tong Shu led the charge. We explore how the active, crowded intracellular environment influences biology. Here's a link for free access until July 25! RT plz! authors.elsevier.com/a/1hCLP3QxxSr7…

@norascience1 Can you buy me one too? 🥺

@WarmingHannah Why not both? Long recordings are always their own special achievement, and you can likely get multiple recording types out of it as well. We're probably saying the same thing, but I might be a bit dense this morning.

See also:

Analysing spontaneous currents, channelrhodopsin-induced synaptic currents, mapping interneuron input location, and recovering morphology all from the same neuron - what a way to feel productive 🤩 #electrophysiology

@Timothy0Leary Generally, simple methods are more than sufficient.

I offered a masters project on BMI recently. After I outlined it to a few eager students, they asked about the decoding algorithm and were visibly bewildered and/or horrified that it didn't involve "AI" or neural networks.

@SaveTheBees2024 @nixedPoE @robertskmiles @Jess_Riedel Exactly like that. They're all complex systems.

@nixedPoE @robertskmiles @Jess_Riedel Kinda like the weather, too?

I think most people (quite reasonably) think "We built ChatGPT, so we must basically understand how it works" This is not true at all. Humans did not build ChatGPT. In a way it would be closer to say we 'grew' it. We have basically no idea how it does what it does.