Harriet

@UCDflowerpower Depends on the type of population (dh/ril/etc), recombination fraction, and bulk size - number of markers is not usually the limiting factor. Happy to chat if you want send me a DM 😊

Ok, quantitative genetics folks, really dumb question: Can one estimate the effect size of a QTL from a bulked segregant analysis? If this is not possible, how many markers does one minimally need to estimate effect size?

@easyJet for the love of god can someone please reply to me

@easyJet hello???????

.@easyJet is there any chance that someone could respond to my baggage claim from APRIL?? You lost it, so at least have the courtesy to communicate with me. I’ve been ignored since May. I know you’re busy but cmon now… I’ve had to fork out to replace everything 😞 #notgoodenough

@IrishRail am trying to book a train but page crashes after I put card info in! Can I book over phone?

A great #Easter! First @alex_perochon and my paper on #fusarium responsive gene clusters in wheat nature.com/articles/s4159… And then @sobia_ajaz on susceptibility to #septoriatriticiblotch in wild and ancestral wheat lines bsppjournals.onlinelibrary.wiley.com/doi/10.1111/pp… @doohancropslab

@TheHillsCountry Milk burnt to the bottom of a pan 🙈

Is there a substance known to man, grippier than the layer of scrambled egg left in the bottom of a saucepan? 🧐

Brilliant

@ChrisJ_Burt @Rothamsted @BristolUni @ragtseedsuk What an absolutely brilliant group of supervisors!!!!! That’s one lucky student.

Fantastic opportunity for an iCASE PhD studying barley yellow dwarf virus in wheat @Rothamsted @BristolUni with application into wheat breeding @ragtseedsuk. Guaranteed some excellent supervision (and some ramblings from myself) swbio.ac.uk/files/2020/10/…

@UCDflowerpower What is the answer??? Assistant, associate or in some places ADJUNCT professor??!!?!!

Until I started as an assistant professor I had no idea which level is more senior: assistant or associate.

@popupcamptrout @magiovan @UCDflowerpower There are more plant based assays now available on the bioanalyzer - they are more appropriate for the peaks that you expect to get in plant tissue! Made a huge difference in RIN and ratio values when we used the generic eukaryotic assay vs plant assay!

@magiovan @UCDflowerpower Also bioanalyzer RIN values are very mammal-centric. Plants have extra peaks from chloroplast Ribosomes that result in a poor RIN from perfect RNA. Also many hemipteran insects have extremely wierd ribosomal RNA.

RNA quality test for RNA-seq and qPCR. What should we use?

@UCDflowerpower Nanodrop and bioanalyzer! Depending on the tissue, the nanodrop is important (for example with starchy tissue, the starch co-precipitates with rna and nanodropwill show this). But for degradation, you need bioanalyzer! Also required by most rnaseq companies too!

@hannah_wht @UCDSBES @UCDEarth Judas. But also well done and you did UCD proud!! Can’t wait for the next UCD TCD drinks when we are finally allowed!!!

@spleonard1 But.... but the lids though 😭😭😭😭😭

Yes it’s balanced

Hey #AgTwitter I made this lift for my Dad a couple of years ago so he could continue to run the combine. He is no longer physically able to. If this would help any older farmers I would love to see someone benefit from it. @TheChadColby @MachineryPete @JohnDeere @AaronFintel

@ETSshow Crocheting teddies and trying to teach my carnivorous farmer father what tofu is

Fun question for a Sunday afternoon What is a subject outside of your professional work that you could give a 15 minute talk on right now?

@c_hertweck @jacquimonaghan I’ve had a paper rejected from a frontiers journal......

@joanna_kacprzyk I have a genome joke but it’s really long

I had an apoptosis joke, but I killed it.

Green olives stuffed with garlic and jalapeños are life changing

@fiona_belbin github.com/hbenbow/RNAseq…

#R is driving me insane. Trying to get significance levels from Tukey added as labels on a boxplot. Tried several online solutions but can't get it to work with my data (with the correct lettering). Any useful suggestions?