Imaging_Artifact

Want to ask a #CryoEM #TeamTomo or #ElectronMicroscopy question anonymously? Send an email to imagingartifact@gmail.com to have it shared from this account.

@therickybaker @ImagingArtifact I think it's ODO1: 2-oxoglutarate dehydrogenase(E1)! We recently solved the structure of this contaminant in our dataset (E.coli, His purified contaminant) and found out it was this. Here's 2D templates of our volume:

We are struggling to identify these class averages in a #cryoEM dataset. Does anyone recognize this protein complex from E. coli? Looks to be C2. Doesn't look like the usual culprits (ArnA, GroEL, Catalase, etc.). Sadly preferred orientation so can't get a useful map

GoldGrids Day! 👇🏼Before gold evaporation

Had a rare view 🔍 into the @thermofisher #Krios autoloader, when our #FSE changed the parts. Lasted ~656 cycles since the 2018 installation @ismb_london / @BirkbeckUoL 🦉#cryoEM ❄️🔬facility. Fascinating system that enables automation across TEM for life sciences.

🤔🧐

If you're not on the multi-thousand-person cryo-EM listservs... we (EZ lab @PrincetonCS, @FlatironInst, #CCPEM) just announced a new #heterogeneity challenge for cryo-EM! #CAHRA2025 Webinar this Fri Nov 14 at 12p ET. Datasets and more info here: heterogeneity.notion.site/challenge We've been preparing this challenge for over a year 🥵 (designing the heterogeneity, collecting data, validating results and metrics). Super excited to finally share it with the community! There are three mystery❓❔❓ datasets! Some fun facts about each: 1) comp-het: an experimental dataset containing a mixture of mystery❔complexes 2) conf-het: we're asking folks to submit *atomic models* instead of density maps for the first time ❗️❕ 3) pose-entanglement: a simple dataset to test how much the pose distribution influences conformation estimation 🐎 Huge shoutout to the members of my group driving this work @FeathersRyan and Robert Heeter, @sonyahans + @PilarCossio2's groups @FlatironInst, and Joel Greer from Tom Burnley's group at #CCPEM. Calling both methods developers and users to participate! Check out our website for more details!

That was a 2/3! Thanks to @nm_razad @Pennywang925627 @valendraica for their help, @ImagingArtifact for reposting, and all for their nice suggestions. Still one average missing, and I seriously doubt it is our sample.

Common E. coli contaminants. Thanks for sharing! @nm_razad

Happy Halloween everyone! 🎃

@ChavesSanjuan Given these 2D classes, 3D should produce something reasonable if you can figure out the right symmetry. Possibly D2?

Please, #cryoEM community (and @ImagingArtifact), we need some help to identify some objects in our 2D averages. Probably, contaminants from e coli recombinant protein production. Any idea?

If you are looking for some TEM-related parts, check it out: ebay.com/usr/ucsc-cryoem

Successful replacement of the humidity inlet 🚿 for the TFS/FEI MARK-IV Vitrobot @ismb_london / @BirkbeckUoL 🦉 #cryoEM ❄️🔬. I Never realized how worn and dirty 🦠 these components can get. Working a lot better now, hopefully holds up until the MARK-V is out 😉.

When particles have orientation bias, the resulting maps are often streaked along the missing directions. In this case study, we cover why orientation bias produces anisotropic #cryoEM maps and how you may be able to recover from it! guide.cryosparc.com/processing-dat…

Reprocessing some old #cryoEM data from my time in @aleschziner lab. Gonna use this to prove to my students that I used to be awesome in the lab! 2.7 Å from 550 micros, hand-selected in Leginon and collected in super-res (gasp!) on a Talos with a K2. Ancient by today's standards

Windows has come a long way! Relion 5.0 compiled fine in the WSL-2 and now runs natively in Windows.... Very cool! #cryoem

Good day @ismb_london brining back the FEI Tecnai T10 & T12 electron microscopes 🔬with a O-ring fixes, filament change, and replacement of the high tension tank (easy lift) with @thermofisher engineers. #cryoem.

Running an early 2000s version of #xmipp and #chimera on a 1996 Silicon Graphics Indigo² workstation. These pieces of software have stablished the foundations for many modern #CryoEM image processing packages.

Received some nice hardware from @SubangstromLLC to increase #cryoEM ❄️🔬storage for our users @BirkbeckUoL - @ismb_london. 4 grids per grid box 📦, 12 grid boxes per puck 🏒, 10 pucks per cane. That is 120 grid boxes or 480 grids in a cane - should keep the Krios busy. Hopefully moving away from those 50ml falcon tubes.