Ivan Kuznetsov

53 posts

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Ivan Kuznetsov

Ivan Kuznetsov

@IvanKuznet

Cards fellow @UCSFCardiology || Prev IM resident @PennIMResidents & MD/PhD @PennMedicine @pennbioeng || Engineered biology to study metabolic disease.

Katılım Eylül 2019
401 Takip Edilen139 Takipçiler
Ivan Kuznetsov retweetledi
Jay Giri
Jay Giri@jaygirimd·
🧵 Many “hot takes” about the reduction of @NIH indirect grant costs to 15%. Let’s lay out the facts about the university grant management process & accounting. And then use this to project likely short and longer term impacts of the policy.
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NIH
NIH@NIH·
Last year, $9B of the $35B that the National Institutes of Health (NIH) granted for research was used for administrative overhead, what is known as “indirect costs.” Today, NIH lowered the maximum indirect cost rate research institutions can charge the government to 15%, above what many major foundations allow and much lower than the 60%+ that some institutions charge the government today. This change will save more than $4B a year effective immediately.
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Prof. Nikolai Slavov
Prof. Nikolai Slavov@slavov_n·
This rate of validation suggests that antibodies should be considered non-specific until proven otherwise: 357 validated out of 1,124 tested. .
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Feng Zhang
Feng Zhang@zhangf·
Since the discovery of CRISPR-Cas systems, people have wondered if a similar type of system exists in eukaryotes. Today, we report in @Nature the characterization of Fanzor, a eukaryotic RNA-guided system that can be reprogrammed for human genome editing. nature.com/articles/s4158…
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Mikhail Shapiro (same on bsky)
Mikhail Shapiro (same on bsky)@mikhailshapiro·
Can you express multiple genes from a single mRNA at programmable ratios without long intervening sequences or peptide overhangs? 🤔 Yes, with SEMPER! 😉 Congrats @mtduan, @ishaanjdev and coauthors on this new tech for mammalian synbio & gene therapy. biorxiv.org/content/10.110…
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Alice Ting
Alice Ting@aliceyting·
LOV-Turbo is published today in @naturemethods and plasmids for LOV-Turbo are available on Addgene, here: addgene.org/browse/article… We hope many people will use LOV-Turbo in exciting new ways! nature.com/articles/s4159… If you plan to use NanoLuc BRET to activate LOV-Turbo, please reach out, as we are engineering improved LOV-Turbo + NanoLuc variants with higher BRET efficiency.
Alice Ting@aliceyting

Light-regulated TurboID, or LOV-Turbo, is a feat of engineered allostery, created by @SongyiLee10 and @CheahJoleen via structure-guided design, then optimized by yeast display directed evolution. Activity is nearly undetectable in the dark state, but increases within seconds to a level comparable to TurboID after weak blue-light illumination. Removing light shuts LOV-Turbo off again (ie, it is reversible). LOV-Turbo is easy to use in mammalian cells, yeast, bacteria, and even in the mouse brain to suppress background labeling from endogenous biotin, to perform pulse-chase proteomics, and to spatially control biotinylation. We also show that instead of external blue light, LOV-Turbo can be activated by genetically-encoded light, ie bioluminescence from luciferase, via BRET (Figure 4F-I). Huge thanks to incredibly talented co-first authors @SongyiLee10 and @CheahJoleen, and our wonderful collaborators Steve Carr and @namude. Also many thanks to co-authors @Simenzhao, Charles Wu, @heegwangroh, @tinakim_neuro and @kelvinfcho for their contributions. Plasmids will be available on Addgene in the near future! biorxiv.org/cgi/content/sh…

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Joachim Goedhart
Joachim Goedhart@joachimgoedhart·
Finally out: mScarlet3 A brilliant and fast-maturing red fluorescent protein. Congrats to the teams of Dorus Gadella and Antoine Royant! nature.com/articles/s4159…
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Ali Max Erturk
Ali Max Erturk@erturklab·
Imagine you could stain whole adult mice with standard IgG antibodies. What you couldn't do! We present wildDISCO technology for this🤗, creating the first whole body vascular, lymphatic, and nerve atlases. By @HongchengM @jieluo692 biorxiv.org/content/10.110… 🧵👇🏼(turn on the sound)
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Kristin Knouse
Kristin Knouse@kristinknouse·
Thrilled to share our lab’s first paper in which we establish genome-wide CRISPR screening within a single mouse! We are so excited about the power of this platform to enable high-throughput genetic dissection of diverse phenomena within a living mammal. cell.com/cell-genomics/…
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Vamsi Mootha
Vamsi Mootha@VamsiMootha·
Delighted to introduce SNORCL, a genetic tool for on-demand generation of localized oxygen inside living cells. We harness the power of genetics to express a xporter (NIS) and enzyme (Cld) that together yield SupplemeNtal Oxygen Released from ChLorite. tinyurl.com/54wre8nx
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Keşfet

@NIH @MinaXu7 @YiXing77 @DrMingyaoLi @JunLuLab @dingyao_zhang @MarkGerstein @Nature