Joe Muldoon

Thrilled to report in @natBME our new effort to improve the manufacturing and access of gene edited T cells. Awesome collab with the @RossWilsonLab and @MarsonLab. We developed a protein-based delivery for CRISPR systems in human primary cells. A 🧵 by Ross 👇🏼

I’m excited to share our report - out now in @natBME - of a new approach to CRISPR delivery that is poised to transform the way engineered T cell therapies are manufactured. Visit tinyurl.com/CRISPR-PERC for “how to” details. It’s threadin’ time! (1/11) nature.com/articles/s4155…

Thrilled to share the first Eyquem lab paper! Today we report in @CellCellPress evolving the AAV6 capsid to enable efficient engineering of murine T cells. An exciting collab with @asokanduke and led by the brilliants @WilliamNyberg and Jon Ark cell.com/cell/fulltext/… 🧵1/11

Excited to share our paper @CellCellPress tinyurl.com/22s9pztn. Ray Liu and Iowis Zhu led the development of clinically optimized and tunable nextgen synNotch receptors dubbed SNIPRs for cell therapies. Thank you @UCSF @GladstoneInst @parkerici press: tinyurl.com/2p8usz7z

Today we report in @NatureMedicine the HLA-independent TCR (HIT). Using CRISPR knockin, we remodeled the TCR specificity to target cell surface antigen in an HLA-Independent fashion and benefit from the TCR's unique sensitivity. 1/4 nature.com/articles/s4159…

HLA-independent #Tcell receptors, in which the heavy and light chains of a CAR scFv are incorporated into the endogenous TCR locus, are more effective than #CD28-based #CARs at targeting #tumors with low antigen expression. #leukemia #AML @sloan_kettering nature.com/articles/s4159…

Check out GAMES - a model development workflow for rigorous characterization of synthetic genetic systems! pubs.acs.org/doi/10.1021/ac…

Are you interested in using mathematical modeling to help understand, refine, or employ your synthetic biology system, but don’t know where to start? Introducing GAMES—a model development workflow for rigorous characterization of synthetic genetic systems.

Excited to share new work w/ co-lead @CamilliaAzimi, @MarsonLab @kickassscience1 @roybal_lab: using a method we call 'CAR Pooling', we tested many CARs in multiplex & found new non-T cell signaling domains which improve CAR-T efficacy. biorxiv.org/cgi/content/sh… 👇🧵 1/10

Very excited to be part of the Mnemo adventure and humbled to be a co-founder next to my former mentor Michel Sadelain, Isabelle Rivière and Sebastian Amigorena. endpts.com/michel-sadelai…

Thanks @NIBIBgov for highlighting this cool project led by @JoeJMuldoon with @BagheriLab @NUSynBio nibib.nih.gov/news-events/ne…

1/4 Excited to share our new preprint from the @roybal_lab & @MoKhalilLab-Raymond Liu and Iowis Zhu led the development of NextGen synNotch receptors. SynNotch is one ‘species’ in a larger ‘genus’ of SyNthetic Intramembrane Proteolysis Receptors (SNIPRs) tinyurl.com/7kf6bdyr

1/4 We’re excited to share two major papers out @ScienceTM showing the many benefits of synNotch CAR circuits for the treatment of solid tumors. Axel Hyrenius-Wittsten, an incredible postdoc @roybal_lab, led the studies in Ovarian Cancer and Mesothelioma. tinyurl.com/drje876u

Our new study on predictive design of mammalian genetic programs is out in @ScienceAdvances! We paired engineered genetic parts that have special capabilities with computational models to design circuits that perform sophisticated, useful functions advances.sciencemag.org/content/7/8/ea…

Nice piece on predictive design of mammalian genetic programs - a great collaboration with @BagheriLab led by @JoeJMuldoon. Beautiful art by Justin Muir. @NUSynBio @NIBIBgov @ScienceAdvances advances.sciencemag.org/content/7/8/ea… news.northwestern.edu/stories/2021/0…

Looking to obtain COMET or mMoClo plasmids from @Addgene ? We have deposited 291 plasmids and they are starting to become available on @TheLeonardLab Addgene Page. See thread below for more information and links:

Need a stocking stuffer? Our study on elucidating and refining synthetic receptor mechanisms is out in final version today @OUPSynBio! @NUSynBio @NorthwesternEng doi.org/10.1093/synbio…

A powerful approach for controlling delivery at the single cell level: this was a fun collaboration with @Espinosa_HDE, Jack Kessler, and teams @JoeJMuldoon @NUSynBio

Now on bioRxiv—we build upon and combine several mammalian syn bio technologies. Our approach enables the construction of a wide range of genetic programs, and it makes the leap from relying upon intuition to using accurate model-guided design. Very proud of the team.

Congratulations @JoeJMuldoon (@TheLeonardLab and @BagheriLab) on your thesis seminar today! #IBiSGrads #IBiSAlumni