Technical Specialists , check this webinar for career advice. Will definitely look into the 'Technician Commitment' , action plans and opportunities to build confidence/responsibilities. Cheers @YorkBioimaging tinyurl.com/mk5bwuua
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Michael Nesbit
104 posts
Michael Nesbit
@MLNesbit
Senior Tech for Microscopy Facility @CHIRI_curtin, Perth, Aus. Also RO investigating cerebral capillary integrity in ageing and neurodegenerative diseases.
Perth, Australia Katılım Ekim 2020
372 Takip Edilen236 Takipçiler
This was really useful for a lonely facility staff member!
There's no manual for this so it's important to hear from experienced peers and was great to meet you all #LMA2024
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Attended the Core Facility Managers Workshop at #LMA2024, headed by @DrPaulMcMillan. It was good to see how other facilities are run and organized. I've picked up a few good things I can implement in my facilities to hopefully improve the user experience.
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@YorkBioimaging @zeiss_micro Good luck boss
Otherwise see me on ebay ;)
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@FA_Olivier @zeiss_micro If you were at the dinner... each table had one... maybe there's more
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@MLNesbit @zeiss_micro Omg was that a prize, or do they have a few more available?!
Melbourne, Victoria 🇦🇺 English
🔬 🔥🔬 🔥 I've been granted release from the lab!
I'm in Melbourne for #LMA2024.
(Light Microscopy Australia conference)
Come check out my poster on Thursday, or just see below and say HI
🔬 🔥 🔬 🔥
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Congrats @CHIRI_curtin image of the month winner Dr Gae Ellison!
Iron (ferroorange) in neuroblastoma cells, rendered using spots and surfaces in @ImarisSoftware
Captured using @AndorTechnology Dragonfly using @NikonInst 60x lens
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🧠Please watch - 1 minute - Visualise Your Thesis! Amazing winning entry from our final year PhD student Arazu! @CHIRI_curtin @CurtinMedia
Blood-Brain Barrier Integrity in Diabetes-Induced Alzheimer's: Investigating the Effect of Peripheral Amyloid-beta doi.org/10.6084/m9.fig…
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@NerdyCammy @ilastik_team Awesome thanks Cam, will give it a go next time! Haven't played much with binary calculations in Fiji but definitely need to learn
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@MLNesbit @ilastik_team I haven't used local thickness before. But to select stuff like I have I think the following would work
- EDM of your binary
- minimum calculation between the EDM and your skeleton
- threshold result to select skeletons of desired size
- make new binary
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Ah Euclidean distance, is there anything you can't do?
A binary blob + EDM + Skeleton = average width of blob arms :)
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@NerdyCammy @ilastik_team we were pretty happy with the Labkit segmentation step, but the issue with Local Thickness was it normalising to the thickest diameter in each image, rather than a standard.
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@MLNesbit I think this is a case where @ilastik_team is your answer to get good thresholding for the skeletonise step. Shoot me one of your images and I can have a play.
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@CityofPerth hello, who looks after the digital tower @ Yagan Square?
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@NinaTParker My friend used to go as a Muggle from Harry Potter.
No effort necessary!
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Image Competition - open to CHIRI members using any facility AND external members using CHIRI equipment!
Learn about our Institute:
🏠 research.curtin.edu.au/chiri/
🔬 research.curtin.edu.au/chiri/our-faci…
🖥️ @CHIRI_curtin
🇦🇺 Perth, WA
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well done Nathan! our first winner to use 60x!
nice objectives @NikonInst and confocal @AndorTechnology
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Great work Virginie and Tim! @CHIRI_curtin #ImageOfTheMonth
Fantastic colab with @NIFAus and @CMCA_UWA
Australian National Imaging Facility@NIFAus
Congrats to @CMCA_UWA NIF Fellow @DrTimRosenow and Dr Virginie Lam on being awarded @CHIRI_curtin's Image of the Month! The high-res DTI with white matter fibre-tracking, overlaid on a structural image, was expertly produced by Tim on UWA's 9.4T preclinical MRI.
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@DaniBeckman @zeiss_micro That's good to hear thanks! Yes we do mouse and rat , works great with snap frozen (post fix on slide) but not at all with PFA / sucrose freezing, no perfusion
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@MLNesbit @zeiss_micro Yeah, these are not frozen sections. We keep them at 4 degrees max. But I think the most important factor for vascular markers is how you process the brain. Is it a rodent model? Perfused?
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The photo below shows an excellent combination to study blood-brain barrier integrity: claudin 4 (blue) and gfap (red/yellow). @zeiss_micro
Is #MicroscopyMonday! Share any microscopy you have; it doesn't have to be fluorescence! Let's make Twitter prettier 😁 🔬 #NeuroTwitter
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