Microscopy Memes 🔬

Why do my cells look blurry?

Testing oil objectives with spring-loaded nose cones

New programmers adding comments

Bring a bucket and a mop for this #NanoPyx

me (lactose intolerant): Do you have almond milk? Technician making blocking buffer: what

Biosafety officers looking at this thread

Most people don't pay attention to acquisition order on #confocal #microscopes. Frequently, nuclear stains like DAPI are the first channel. Instead, let the labelling inform you. For example: Channel 1: Desmoglein 1 Channel 2: Plakophilin 2 Channel 33342: Hoechst 33342

@mcammer1 I used a repository provided by my university, so it won't help you. But check out twitter.com/CiminiLab/stat…

@MicroscopyMemes Which respository? Writing a guide for PIs for new DMP policy.

When your large microscopy data finally uploaded to the data repository and you click on a different section before saving

Thanks Pfizer

When I'm checking alignment of the STED scope and Jerry asks what I'm imaging

When someone says "statistics" in response to "What are you looking to get out of these images?"

Surely this'll convince sCMOS manufacturers to do something.

What do you do after justifying aims? #grantwriting

When you interrupt someone mouth pipetting

Me to my cells as I optimize live-cell imaging conditions

@RunningPhoton @microRussell @JamesDManton @AndrewGYork @FOMconferences

At which iteration would you stop this image restoration? Comment below.🤔🔬 me, @microRussell, @JamesDManton, @AndrewGYork had an idea, which I am going to present at #FOM2022 | @FOMconferences on Tuesday at 13:25 (CET) in "Image Analysis Methods"

When deciding which objective to purchase, save time by buying one which has already been scratched.

How #STED microscopy works

Actual footage of the fast moving diffraction-limited object that I want to FRAP.

Every paper on modifying fluorophores be like