Mihail Todorov

🧠Bad vascular aging ≠ vessel loss - it's a fork in the road. Some paths preserve function, others enter a hypervascular, BBB-leaky state tied to memory impairment🔬. Why do some aging brains stay resilient while others decline?🤔 🔍Some clues in our new preprint🧵👇

Does brain aging always mean vessel loss? 🧠We found a surprising hypervascularization, driving cognitive decline & blood-brain barrier damage in aging. 👇🧵biorxiv.org/content/10.648… Led by @MihailMuc

Thrilled to share our work on DISCO-seq, which combines RNA-preserving tissue clearing with whole-organ 3D imaging to enable anatomically unbiased single-cell transcriptomics, with applications in cancer & infection biology. #SpatialBiology #SingleCell #Transcriptomics #DISCOseq

In this study we show that FOXF2 maintains cerebrovascular function via Tie2 signaling, and that pharmacological activation of Tie2 with AKB-9778 rescues deficits in cerebral small vessel disease and stroke. You can read the full story here: nature.com/articles/s4159…

Most transcriptomics studies sample ~0.001% of a mouse. Which region to choose? Often we guess. We introduce DISCO-seq🤗flipping the order: Whole-body 3D imaging with RNA-preserving clearing→ pick ROIs in 3D→ unbiased scRNAseq Led by @HarsharanBhatia biorxiv.org/content/10.648…

@katalin_volgyi @Judit_GonzalezG @NatureNeuro I'm very happy and greatly thankful for the opportunity to team up with such amazing scientists to elucidate novel mechanisms in stroke and cerebral small vessels disease. A spot on use case for VesSAP - our deep learning method for whole-brain unbiased vascular analysis. 🧠 🚀

🚀 We’re hiring! Staff Scientist / Postdoc – Tissue Clearing & 3D Image Analysis (m/f/d) (LMU Munich) Are you a great fit, or do you know someone outstanding, please reach out 🔁 If you want to at the frontier of whole-organ / whole-body 3D imaging, and help generate truly beautiful datasets that drive major biological discoveries and therapeutic development, see below ✨ We’re building the next-generation pipeline for tissue clearing + light-sheet microscopy + quantitative 3D analysis in the SyNergy Excellence Cluster (Mesoscale Hub) and we’re looking for someone excited to push this forward with us. 🧠🔬📈 🎥 I’m also attaching a short video showing the kind of high-quality imaging and datasets you’d be working with. What you’ll do 🛠️ 🔹 Lead and evolve tissue clearing + light-sheet workflows across collaborative SyNergy projects 🔹 Turn complex 3D datasets into robust quantitative insights (visualization, atlas registration, readouts) 🔹 Develop new methods and analysis pipelines together with our AI team 🤖 🔹 Maintain and optimize cutting-edge light-sheet systems (optional: support animal license writing) What we’re looking for 🎯 ✅ Strong hands-on experience in tissue clearing and/or fluorescence microscopy ✅ Solid experience with light-sheet microscopy and 3D imaging workflows ✅ Familiarity with 3D tools like Imaris / arivis Vision4D, stitching (e.g., BigStitcher), and quantitative analysis in cleared tissues ✅ Service mindset, great organization, and strong scientific English How to apply 📩 Apply via the LMU Klinikum online application form lnkd.in/gCgdaJuV Please also send your application to: farida.hellal@med.uni-muenchen.de CC: ali.ertuerk@med.uni-muenchen.de 📎 Include one PDF: short cover letter, CV, 2–3 referees, and earliest start date. 📍 Campus Großhadern (Munich) and Helmholtz Munich | 🕒 Full-time | 📅 Start: 01 January 2026 If you love high-quality imaging, cutting-edge biology, and building something that will matter, we’d love to hear from you. 🌍✨ #hiring #StaffScientist #Postdoc #TissueClearing #LightSheetMicroscopy #ImageAnalysis #SpatialBiology #Neuroscience #SyNergy #LMU #Munich

@katalin_volgyi @Judit_GonzalezG @NatureNeuro I'm very happy and greatly thankful for the opportunity to team up with such great scientists to elucidate novel mechanisms in stroke and small vessels disease. A spot on use case for VesSAP 🧠🚀

We discovered that the skull bone marrow has direct connections to brain surface & mirrors brain inflammation, making it a new site for diagnosing & monitoring brain pathologies. Kolabas…Ertürk, Cell: cell.com/cell/pdf/S0092… biorxiv.org/content/10.110… bit.ly/3PQuymm

We have developed MouseMapper AI, a deep learning ensemble for the analysis of whole-body systems, including the nervous and immune systems, to map disease/drug perturbations tissue by tissue, organ by organ, at the cellular level in 3D. MouseMapper uncovered obesity-induced whole-neuronal changes, most notably, a loss of trigeminal nerve endings linked to impaired whisker sensitivity, as well as tissue-specific macrophage clustering across the body. Kaltenecker, Horvath, Al-Maskari, Kolabas, Chen… Ertürk, bioRxiv (2024) biorxiv.org/content/10.110…

Excited to share our latest work with Denali Therapeutics as Deep Piction. Using our proprietary clearing & imaging, we’ve mapped the full targeting profile of brain-penetrating transport vehicles (ATVs) in both whole mouse bodies and monkey brains. nature.com/articles/s4146…

See our recent @NatureBiotech study SCP-Nano in flow! Mapping LNPs, AAVs, and DNA nanostructures to track targeting & unseen toxicity before clinical trials 🤩. Already >190K access, the most in recent years! 👇🏼 nature.com/articles/s4158…

LNP/mRNA therapeutics and AAV-based approaches require cell-level precision to achieve success without significant toxicity to other cells. To enable this, we now introduce SCP-Nano🤗 in @NatureBiotech: an AI-based technology for precision nanotechnology. nature.com/articles/s4158…

SCP-Nano: Advancing Precision Medicine with Nanocarrier Visualization How can life-saving therapies reach their target cells without harmful side effects? 👉t1p.de/muluc 💡Researchers at #HelmholtzMunich, @LMU_Muenchen, and @TU_Muenchen have developed SCP-Nano (Single-Cell Profiling of Nanocarriers): The new technology visualizes nanocarriers in the entire mouse body at a single-cell level. 🔬 This innovation combines advanced imaging and #AI to detect even ultra-low doses of nanocarriers like lipid nanoparticles, DNA origami, and AAVs. SCP-Nano paves the way for safer and more effective mRNA vaccines, gene therapies, and beyond. 🌟Why it matters: SCP-Nano ensures therapies are delivered to the right cells while identifying harmful off-target effects - driving the future of personalized medicine and precision drug development. @erturklab @jieluo692 @SyNergy_Cluster @NatureBiotech #Nanotechnology #PrecisionMedicine #DrugDevelopment #AIinHealthcare #mRNATherapies #GeneTherapy #PersonalizedMedicine

Our new study shows that SARS-CoV-2 spike protein accumulates & persists in the body for years after infection, especially in the skull-meninges-brain axis, potentially driving long COVID. mRNA vaccines help but cannot stop it🔬🧠🦠🧵👇@cellhostmicrobe cell.com/cell-host-micr…

ErtürkLab is now on BlueSky! 🦋 We’re exploring new spaces for science communication. Follow us for our updates on AI & tissue clearing-driven whole body imaging for cellular-level disease research: bsky.app/profile/erturk…

Thrilled to announce that I will be starting as an Assistant Professor at Cornell University in February! #MICCAI #miccai2024 linkedin.com/posts/johannes…

Imagine seeing a disease's impact on every cell in the body. Now, you can! We introduce MouseMapper, an AI tool to reveal system-wide disease perturbations. MouseMapper showed obesity-induced changes in facial nerves & body-wide inflammation 👉🏼🧵biorxiv.org/content/10.110…

What if we could map every cell in the body, revealing its location and molecular identity? In this @NatureMethods perspective, we discuss the new era of 3D-omics by tissue clearing and AI, called Deep 3D Histology: rdcu.be/dNBe8 PDF: bit.ly/3WaD0QU 🧵👇🏼

The July issue is live today! nature.com/nmeth/volumes/… The beautiful cover comes from the @erturklab and shows c-Fos+ neuronal mapping in whole mouse brains. See paper: nature.com/articles/s4159…