Bryan Johnson@bryan_johnson
Doing magic mushrooms this Sunday.
Protocol
+ dose: 5 grams
+ frequency: 1x/mo for 3 mo
+ objective: exploring longevity effects
Measurement
+ 249 independent biomarkers
+ 29 vials of blood
+ brain scans
+ urine, stool, saliva, fertility
+ multi-omics profiling (DNA, epigenetics, metabolism, hormones, microbiome, proteins, cognition)
Cardiovascular
+ LDL-C
+ HDL-C
+ Triglycerides
+ Total cholesterol
+ Apolipoprotein B
+ Apolipoprotein A-1
+ Lipoprotein(a)
+ LDL-P
+ HDL-P
+ Small LDL-P
+ LDL size
+ Large VLDL-P
+ Large HDL-P
+ VLDL size
+ HDL size
+ LP-IR score
+ Oxidized LDL
+ Lp-PLA2 activity
+ Myeloperoxidase (MPO)
+ Homocysteine
+ VEGF
Hematology
+ WBC
+ RBC
+ Hemoglobin
+ Hematocrit
+ MCV
+ MCH
+ MCHC
+ RDW
+ Platelets
+ Neutrophils
+ Lymphocytes
+ Monocytes
+ Eosinophils
+ Basophils
+ Immature granulocytes
+ Erythropoietin (EPO)
+ ABO/Rh typing
Hormonal
+ Thyroid panel with TSH
+ Free T4
+ Testosterone
+ Dihydrotestosterone (DHT)
+ 5α-DHT
+ Prolactin
+ Estradiol
+ LH
+ FSH
+ Estrone (E1)
+ Estriol (E3)
+ Androstenedione
+ Androsterone
+ 5β-Androstanediol
+ Etiocholanolone
+ Cortisol
+ Cortisone
+ DHEA
+ DHEA-S
+ Pregnenolone
+ IGF-1
+ Melatonin (6-hydroxymelatonin sulfate)
Metabolic
+ Glucose
+ Insulin
+ Hemoglobin A1c
+ α-Hydroxybutyrate
+ β-Hydroxybutyrate
+ Uric acid
Kidney
+ BUN
+ Creatinine
+ eGFR
+ BUN/Creatinine ratio
+ Cystatin C
+ Urinalysis (specific gravity, pH, color, appearance, leukocyte esterase, protein, glucose, ketones, occult blood, bilirubin, urobilinogen, nitrite, WBC [hpf], RBC [hpf], epithelial cells, casts, bacteria)
+ Albumin/Creatinine ratio
Pancreatic
+ Total protein
+ Albumin
+ Globulin
+ Total bilirubin
+ Alkaline phosphatase
+ AST
+ ALT
+ GGT
+ Amylase
+ Lipase
Minerals
+ Sodium
+ Potassium
+ Chloride
+ CO₂ (bicarbonate)
+ Calcium
+ Magnesium
+ Iron
+ TIBC
+ UIBC
+ Iron saturation
+ Ferritin
+ Zinc
Vitamins
+ Vitamin B12
+ 25-hydroxy Vitamin D
+ Coenzyme Q10
+ α-Tocopherol
+ β-Carotene
+ Glutathione
+ Total glutathione
Amino Acids
+ Cysteine
+ Methionine
+ Taurine
+ Glycine
+ Serine
+ Threonine
+ Alanine
+ Valine
+ Leucine
+ Isoleucine
+ Phenylalanine
+ Tyrosine
+ Tryptophan
Inflammation
+ Interleukin-6 (IL-6)
+ Interleukin-10 (IL-10)
+ Tumor Necrosis Factor-α (TNF-α)
+ Rheumatoid factor (RF)
+ ANA by IFA
+ Calprotectin (GI inflammation),
+ Secretory IgA (mucosal immunity)
Neurology
+ P-tau217
+ Epinephrine metabolites (metanephrine, normetanephrine)
+ Homovanillate (HVA)
+ Vanilmandelate (VMA)
+ 5-Hydroxyindoleacetate (5-HIAA)
Toxicology
+ Lead
+ Lithium
Urology
+ PSA total
Fertility
+ Total Motile Count
+ Sample Volume
+ Concentration
+ Motility
+ Morphology
+ Count
Metabolomics
+ Citrate
+ cis-Aconitate
+ Isocitrate
+ α-Ketoglutarate
+ Succinate
+ Fumarate
+ Malate
+ Pyruvate
+ Lactate
+ 2-Hydroxyglutarate,
+ Hydroxymethylglutarate,
+ 3-Methylglutaconate,
+ 2-Methylsuccinate
+ Formiminoglutamate (FIGLU),
+ Methylmalonate (MMA),
+ Xanthurenate
+ Kynurenate,
+ Quinolinic acid
+ Glucarate,
+ 8-Hydroxy-2′-deoxyguanosine (8-OHdG),
+ Pyroglutamate
+ 3-Hydroxyphenylacetate,
+ 4-Hydroxyphenylacetate,
+ 2-Hydroxyphenylacetic acid
+ 4-Cresol,
+ p-Hydroxybenzoate,
+ Benzoate,
+ Hippurate/Hippuric acid
+ Phenylacetate/Phenylacetic acid,
+ Indoleacetate/Indoleacetic acid
+ Arabinose,
+ Citramalic acid,
+ α-Hydroxyisobutyric acid
Microbiome
+ Bacteroides fragilis
+ Bacteroides vulgatus
+ Faecalibacterium prausnitzii
+ Akkermansia muciniphila
+ Roseburia spp.
+ Eubacterium rectale
+ Lactobacillus spp.
+ Bifidobacterium spp.
+ Escherichia coli
+ Clostridium difficile
+ Enterococcus faecalis
+ Candida albicans
+ Saccharomyces boulardii
+ Prevotella copri
+ Ruminococcus spp.
+ Methanobrevibacter smithii
+ Blautia spp.
+ Proteobacteria spp.
+ Firmicutes/Bacteroidetes ratio
+ Beta-glucuronidase
+ Butyrate
+ Propionate
+ Acetate
Aging & Epigenetics
+ Telomere length
+ Relative telomerase activity
+ DNA methylation age
+ DNA methylation levels at CpG sites associated with biological aging
+ Glucocorticoid receptor methylation (NR3C1)
+ SIRT1 methylation
+ MTOR methylation
+ Oxidative stress response genes (NFE2L2 promoter methylation)
Cognitive
+ Brain age score
+ Functional connectivity strength
+ Global connectivity index
+ Hemodynamic response amplitude
+ Hemodynamic response latency
+ Resting-state oxygenated hemoglobin (HbO) concentration
+ Resting-state deoxygenated hemoglobin (HbR) concentration
+ Total hemoglobin (HbT) concentration
+ Oxygen saturation (O₂Sat)
+ Cerebral blood volume (CBV)
+ Cerebral blood flow (CBF)
+ Neurovascular coupling index
+ Prefrontal cortex oxygenation
+ Parietal cortex oxygenation
+ Temporal cortex oxygenation
+ Occipital cortex oxygenation
+ Task-evoked hemodynamic response (HbO/HbR)
+ Temporal autocorrelation of hemodynamics
+ Functional brain network modularity
+ Inter-regional coherence
+ Brain lateralization index
+ Oscillatory power (slow cortical fluctuations)
+ Phase synchronization index
+ Mean reaction time (during cognitive tasks)
+ Reaction time variability
+ Heart-rate variability (HRV)
+ Resting heart rate
