Nik retweetledi
Streamlining TMT-based multiplexing for #chemoproteomics, reported by @Nik_Burton & @Keribackus
nature.com/articles/s4200…
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Nik
44 posts
Nik
@Nik_Burton
Chemistry Ph.D. Candidate @ UCLA 🧪 NCSU Alum 🐺🚩
Los Angeles, CA Katılım Mart 2020
174 Takip Edilen93 Takipçiler
This reagent isn't just for show either. In a head-to-head comparison with traditional cysteine profiling using TMT we show decreased variance with sCIP-TMT likely due to not having to carry each sample through an individual sample prep. Also saves a lot of pipetting time!
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It's no secret TMT labeling is a bit laborious and we saw an opportunity to streamline this by developing click-compatible isobaric tags which allow for early-stage sample combination (doi.org/10.1021/jacs.3…). However we wanted to make this technology more widely applicable
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Really happy this is out and excited to see it adopted in the click-chemoproteomics space. A short thread on how we converted TMT tags into enrichable, click-compatible isobaric tags.
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Keriann Backus@Keribackus
excited to share our sCIP-TMT manuscript now out in @CommsChem link.springer.com/article/10.103… We've added some neat stuff since the preprint, including n-terminal proteomics. Congrats to @Nik_Burton !
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Nik retweetledi
🥳Congrats to biochemistry undergraduate student Emil Dominguez ’25 (@Keribackus group), chosen for the 2023-24 UCLA EDI Student Leadership Award! @uclaphyssci
chemistry.ucla.edu/news/2023-2024… @EmilRDominguez
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A big congratulations to Katya and her amazing team. The project was a ton of fun to work on, exciting to see it out! In short: state dependent targeting of splicing in T cells leads to ITK degradation/activation suppression through SF3B1 inhibition 1/2
Katya Vinogradova@katenokv
Very excited to share our new @biorxivpreprint! Big thanks to all the co-authors who contributed to this story on Covalent targeting of splicing in T cells! @Sculpturatus, Hiro, Nathalie, @chardw98 @teezhangg @xzhang85 Read more below!
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Nik retweetledi
Amazing @ChemRxiv preprint by @Keribackus. They develop sCIP-TMT tags that allow isobaric TMT labeling for cysteine profiling at an early stage in the workflow with huge potential to increase sample reproducibility and reduce workflow time. chemrxiv.org/engage/chemrxi…
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Nik retweetledi
Excited to share our new preprint: sCIP-ing towards streamlined chemoproteomics. chemrxiv.org/engage/chemrxi…
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Nik retweetledi
Super excited to share our latest preprint and our first deep dive into understanding how cysteine-reactive electrophiles impact the proteome, causing widespread protein degradation, aggregation, proteasome activation, stress granule formation, and more. biorxiv.org/content/10.110…
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Nik retweetledi
Preprint Friday for our lab! Proteoform-selective inhibitors of caspase-2 identified by chemoproteomics and a Tev-activation small molecule screening assay. biorxiv.org/content/10.110…
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Wooooooo
Keriann Backus@Keribackus
Just in time for @Nik_Burton's flash @ChemBioTalks our lab's first @J_A_C_S paper! sCIP custom isobaric chemoproteomics capture reagents. Something for everyone in this paper, new synthesis, FragPipe diagnostic ion mining, new reporters, and cysteine reactive electrophiles.
Nik retweetledi
The second flash talk of the @ChemBioTalks will be given by Nik Burton @Nik_Burton from the @Keribackus lab. He will present his research on “A solid-phase compatible silane-based cleavable linker enables custom isobaric quantitative chemoproteomics”. doi.org/10.26434/chemr…
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Nik retweetledi
We are excited to announce the three winners of the flash talk challenge for the @ChemBioTalks: Rosalie Lipsh @Rosalipsh @FleishmanLab, Nik Burton @Nik_Burton @Keribackus and Janine Gray @ChemGray @TateScience will give insights into their exciting research in a flash talk.
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