OpenCellCZB

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OpenCellCZB

OpenCellCZB

@OpenCellCZB

OpenCell is a collaborative proteome-scale project to measure human protein localizations and interactions led by the @LeonettiManuel lab @czbiohub

San Francisco, CA Katılım Mart 2021
3 Takip Edilen192 Takipçiler
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Marco Hein @marcoyannic.bsky.social
Marco Hein @marcoyannic.bsky.social@marcoyannic·
One of the super useful features of the opencell.czbiohub.org website is that you can look up the cellular copy numbers and concentrations of your favorite proteins - check it out! (even for proteins that aren't among our targets or their interactors)
Marco Hein @marcoyannic.bsky.social tweet media
Manuel Leonetti@LeonettiManuel

Our paper is out today in @ScienceMagazine, I’m stoked @OpenCellCZB ! Check out opencell.czbiohub.org: CRISPR tagging + 3D live imaging + IP mass spec to map the human proteome. science.org/doi/10.1126/sc… 👇 tweetorial from our 2021 pre-print.

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OpenCellCZB retweetledi
elevyLab
elevyLab@ElevyLab·
Check our perspective on the @OpenCellCZB awesome work from @LeonettiManuel "Modules" help interpret biological systems. However, we see in these new data that connectors far outweigh modules in PPI networks! What does that mean? We discuss it: science.org/doi/10.1126/sc…
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OpenCellCZB
OpenCellCZB@OpenCellCZB·
@damiandn We certainly tried! But we've "only" tagged ~10% of the proteome - there's a long way to go, and many cell types to explore (and we hope robots will get us there, since it took a heroic amount of TC by many folks on our team to get us even this far)
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Damian Dalle Nogare
Damian Dalle Nogare@damiandn·
When I was in grad school (pre CRISPR, obvs) a prof once asked what our fantasy experiment was. I said it was to stick a fluorescent tag on every gene in the genome and image the endogenous localization. These crazy bastards have basically done it.
Manuel Leonetti@LeonettiManuel

Our paper is out today in @ScienceMagazine, I’m stoked @OpenCellCZB ! Check out opencell.czbiohub.org: CRISPR tagging + 3D live imaging + IP mass spec to map the human proteome. science.org/doi/10.1126/sc… 👇 tweetorial from our 2021 pre-print.

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OpenCellCZB
OpenCellCZB@OpenCellCZB·
@sciencehacker Thank you! Please let us know if you have ideas about how to make the web app even better - we are always striving to improve it!
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OpenCellCZB
OpenCellCZB@OpenCellCZB·
This is just the beginning! We are continuing to tag new proteins and we are working on a way to distribute our existing library of cell lines. Stay tuned for new releases of our web portal and datasets!
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OpenCellCZB
OpenCellCZB@OpenCellCZB·
All of our data is freely available to explore and download at opencell.czbiohub.org/download. Special thanks to the AWS Open Data program for hosting our collection of 6,000 raw 3D microscopy images: #microscopy/raw/" target="_blank" rel="nofollow noopener">czb-opencell.s3.amazonaws.com/index.html#mic…
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OpenCellCZB retweetledi
Chan Zuckerberg Biohub Network
Chan Zuckerberg Biohub Network@czbiohub·
Published today in Science, a team from CZ Biohub and @MPI_Biochem combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. 🧵 science.org/doi/10.1126/sc…
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OpenCellCZB
OpenCellCZB@OpenCellCZB·
Excited to see this update! Cytoself enabled us to quantitatively compare our IP/MS interaction data with our microscopy images (spoiler alert: we showed that if proteins in our library share similar localization patterns, they are more likely than not to directly interact!)
Hirofumi Kobayashi@liilii_tweet

We have an updated preprint of cytoself, our deep-learning method for profiling protein localization with self-supervised learning! Fantastic collaboration with @kchev, @LeonettiManuel and @loicaroyer. (1/8) biorxiv.org/content/10.110…

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OpenCellCZB retweetledi
Chan Zuckerberg Biohub Network
Chan Zuckerberg Biohub Network@czbiohub·
Cellular life is amazingly complex. To find cures for diseases, we must understand the molecular details of how cells and their proteins operate. Visit our new OpenCell website and interactively explore the localization and interactions of human proteins:opencell.czbiohub.org
Manuel Leonetti@LeonettiManuel

Endogenous tagging + 3D live-cell imaging + mass-spec interactome = opencell.czbiohub.org, an open-source map of the human proteome. So excited for our first release, wonderful collaboration with @labs_mann @loicaroyer & many other colleagues. @OpenCellCZB

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OpenCellCZB
OpenCellCZB@OpenCellCZB·
Our first release is live! 🚀 A collaborative effort led by @LeonettiManuel with @labs_mann and @loicaroyer and many others at @czbiohub to build an open-source map of the human proteome. Check it out and let us know what you think! opencell.czbiohub.org
Manuel Leonetti@LeonettiManuel

Endogenous tagging + 3D live-cell imaging + mass-spec interactome = opencell.czbiohub.org, an open-source map of the human proteome. So excited for our first release, wonderful collaboration with @labs_mann @loicaroyer & many other colleagues. @OpenCellCZB

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Keşfet

@LeonettiManuel @damiandn @sciencehacker @ScienceMagazine @MPI_Biochem @czbiohub @labs_mann @loicaroyer