Sam Old

@wolfgangkhuber Could you use this to host shiny apps instead of using shiny.io?

@Rarghwoolf And what about adults?!

I don’t mind being yelled at when it’s a baby piwakawaka

@schotz Yeah I tried it for some scrna and flow cytometry stuff but it keeps making up random functions or renaming them. You need to spend some time finding the real functions, which is not the best time saver.

Does ChatGPT write R code? Asking for a friend.

Stop using the elbow criterion for k-means and how to choose the number of clusters instead. (arXiv:2212.12189v1 [stat.ML]) ift.tt/TJaIE2X

Be careful with this R stuff kids. It starts with just wanting to analyze some data, then before you know it you're trying to figure out how to deploy dockerized shiny apps.

@bioinformer @PNgsabrina They are zero vs one, zero vs two, zero vs three... counts. Need to not use raw p-value here, lfc shrinkage, more for QC.

Hey #RNAseq folks... what's up with this volcano plot?🌋🧬N=4/condition. Human cells. Ever seen "wings" like this before? #Looksodd2me - but raw data looks great. qPCR confirmed DEGs. Maybe rounding/precision error in pipeline? Maybe I just worry too much? #bioinformatics

@cararthompson I'll try scattermore! I think I previously used geom_hex with a large amount of hexes for >10mil points

Five quick #rstats tips I've picked up in the plots I've made for the first category of the #30DayChartChallenge 🧵👇 1. Need to plot a million points and ggplot() is taking too long but you want to stay within the tidyverse? 👉 Use 📦{scattermore} with geom_scattermore() 🥳

@data_question Wow, I always made ggplots in a for loop, this is way better!

One can use `%+%` operator from ggplot package to replace the current data of plot object with new data

Here are some tips to supercharge your ggplotting skills #RStats (1/n)

@rbjanis5 dplyr::filter I frequently get collision problems with filters from several packages. Also pheatmap::pheatmap()

🥳Finally out!!! 🥳 ... and with a timing that could not have been better (thanks to the editors @BioMedCentral!) to wish me, @AnnekathrinLudt and all of you a pleasant Christmas time - while you wait for Jan2022 to analyze in depth your RNA-seq data🤗🧬🍹 bmcbioinformatics.biomedcentral.com/articles/10.11…

Are you: ✅ An Australian (or NZ 👀) bioinformatics student? ✅ Looking to expand your network? ✅ Keen to organise fun social events? ✅ Passionate about bioinformatics education? Apply to join the COMBINE Committee for 2022! combine.org.au/join-the-2022-…

If the 27+ pages at rdcu.be/cBwr seem too much, here is a summary of the main points 🙂(finally)😉 #Immunology #Allergies #ILC2 @DCell_papers 1/8

Move over banana Venn

Mayer et al. show a homeostatic circuit of IL-13, dermal ILCs and CD11b-lo DC2 cells support the development of TH2 cells and curtails the development of TH17 cells in the skin of mice and humans. rdcu.be/cBzLa @francaronchese @JohannesUMayer

@melissaboardman I ran into a pair on the walk to Butterfly Creek lately! So fun to see them in the wild after following your posts.

Here’s a bundle of cuteness to start your day… These tiny birds are the now famous Te Ahumairangi titipounamu/rifleman fledglings. Here are the 5 siblings having a snuggle 🥰 Only one is male, he has four big sisters! #titipounamu #nzbirds #TwitterNatureCommunity

@kerryhilligan @Malaghan_Inst @royalsocietynz @francaronchese Awesome!

Really excited to share that I’ll be returning to Aotearoa and ⁦@Malaghan_Inst⁩ next year as a Rutherford Fellow! 🎉 Thanks ⁦@royalsocietynz⁩ for the opportunity and my amazing mentors ⁦@francaronchese⁩ and Alan Sher for all your support royalsociety.org.nz/news/2021-ruth…

@mobeginomics @tpq__ So very soon! We are currently going through some revisions before it is published on shinyapps.io

@SameOld_SamOld @tpq__ Are they available for the public. Would love to test drive

@C_L_Bridges @sbrookbrooks Dwindling fan these days, but ye!

Hello! This is a very professional request: If you're still playing Pokemon Go in NZ and consider yourself a "huge fan", I would love to talk to you for a feature I'm doing on the game five years on.

Thrilled to see our recent work featured in a Preprint Watch Journal Club by Mattiuz & @MiriamMerad on @NatRevImmunol !! 😊