NikonImagingUCSD

Yesterday was our first Microscopy Focus Group meeting! Big Thanks to presenter @JuliusBogo1 for sharing his knowledge about microscopy! It was excellent work to design and integrate machine learning processes in high throughput imaging!! @UCSD_NIC @SamReckPeterson @MelsNeurons!!

This Imaging Fellows program is a unique opportunity to gain experience in advanced microscopy core facility management and to pursue a scholarly project in advanced imaging.

We are recruiting! nature.com/naturecareers/…

@rita_strack nor any stop to think

work, work everywhere, nor any drop to drink

@christlet @MadS100tist @Maurice_Y_Lee @ManuelTHERY Totally concur. Make sure you have magnesium but not calcium in the fixation buffer, try a variety of conditions (MeOH, MeOH + PFA, PFA + Glut (with downstream NaBH4 treatments)), and be sure that your fix is mildly basic.

@ericvonotter I think you just described their in house QC protocol.

@ericvonotter Sometimes the most painful lessons stay with us longer.

@ericvonotter Friends don't let friends...

A cool paper from @barrjamie that used copious amounts of confocal imaging to examine the lung-brain connection. journals.physiology.org/doi/full/10.11…

BIG NEWS - Art of Espresso is open again!!! They are opening every day at 8am and closing sometime after lunch. Go there now and show some love!

In case you haven't hear about this through other channels. We're looking for a new director microlist.org/listing/direct…. Come work with a great group of scientists in fabulous La Jolla, CA.

@MarijnSiemons @kschink @florianstroehl @JamesDManton @ASIImaging @Thorlabs This is what I was thinking of. Thanks!

@kschink @UCSD_NIC @florianstroehl @JamesDManton @ASIImaging @Thorlabs Im sharing my build of a snoutscope with Nikon body as base. We have a 3D printed Sacndreas, custom UI and hopefully 10+ vps. Follow along at #aSnoutyTale! 😃

There is an account that has been posting custom printed pieces to build a snouty light path. Could someone direct me to it?

Know someone interested in a light microscopy core facility position? The @UNC_SOM Neuro Microscopy Core is seeking an imaging specialist. Work w the amazing @cziscience imaging scientist @MichelleItano! Join the awesome imaging community @UNC ! unc.peopleadmin.com/hr/postings/21…

@LoogerL @joachimgoedhart @HSchihada @LABrechaun @NathanShaner No doubt. It's great for getting down to molecular mechanisms at the really small scale, but once those are answered you've got to move on.

@UCSD_NIC @joachimgoedhart @HSchihada @LABrechaun @NathanShaner Sure it has throughput! And when you've got an in vitro system, it's great! But so few in vitro systems are representative of in situ...

What CY FRET pair do people currently recommend? mTurquoise2 + mVenusQ69M or Citrine2? @joachimgoedhart @LABrechaun @NathanShaner @LoogerL

@LoogerL @joachimgoedhart @HSchihada @LABrechaun @NathanShaner But you have to love that cys-light maleimide life. A former labmate tested over 600 mutants to find the right labeling pair for doing single molecule FRET on kinesin.

@joachimgoedhart @HSchihada @UCSD_NIC @LABrechaun @NathanShaner no i meant the soooooper-old school way of mutating a single residue to a Cys and then incubating protein with Cys-reactive dye. that's 80% of what my PhD lab did. Super-low throughput, incompatible with living things, blah blah blah

@christlet @NikonInst I love looking at the traces after the fact. Sometimes you can see the training error trace suddenly drop, and it's like seeing the AI's eureka moment. We find that with a Titan RTX we can train a model in about 2:30

Sometimes $0.01 of mounting putty helps a $750k microscope work beautifully.

@IsabellaGaeta1 @TyskaLabActual Lovely confined diffusion/directed transport

what's going on with those puncta 🧐 #CellBiology #Microscopy @TyskaLabActual