Honggui Wu

🚨 Today in @Nature, we report GEMINI—a genetically encoded intracellular memory device that writes cellular dynamics into tree-ring-like fluorescent patterns within cytoplasmic protein assemblies.[1/n] nature.com/articles/s4158…

🤔 “Here we present evidence that eukaryotic circular RNAs (circRNAs) can serve as templates for 3' to 5' backward translation (BT), yielding polypeptides with distinct sequence and structural features not found in canonical proteomes.” biorxiv.org/content/10.648…

Whole-genome single-cell multimodal history tracing to reveal cell identity transition biorxiv.org/content/10.110… #biorxiv_genomic

Spatial methylome + transcriptome.

Our scMicro-C paper is out in @NatureGenet! Huge thanks to the reviewers for their valuable insights, to @XieSunney for unwavering support, and to fantastic collaborators @JiankunZhang21 & @tanlongzhi. Thrilled to share this work!

@super_jrub Can’t agree more!

All I want for Christmas is an air conditioned badminton hall 🥲

Ironman. Maternal iron deficiency causes male-to-female sex reversal in mouse embryos nature.com/articles/s4158… @Nature

In @nature we describe the use of scalable #proteinengineering & #machinelearning to predict millions of bespoke CRISPR-Cas9 enzymes, offering safer & more genome editing tools. 🧬🖥️ @RachelSilvers11 @CGM_MGH @MGH_RI @MassGeneralNews @harvardmed nature.com/articles/s4158…

🔥🔥🔥OUR NEW PREPRINT: Slowing and reversing aging? Nature does it, here we show how! Age deceleration and reversal gene patterns in dauer diapause. Huge congrats to Kristy Totska, João Barata, Walter Sandt and @Meyer_DH biorxiv.org/content/10.110…

Introducing RAEFISH, our lab's new flagship image-based spatial transcriptomics technology that simultaneously enables single-molecule spatial resolution and whole-genome level coverage of long and short, endogenous and engineered RNA species in cell cultures and intact tissues.

We are excited to report the discovery of TIGRs, a widely-occurring RNA-guided system found in bacteria and their viruses. TIGRs consist of a peculiar repeat region which is transcribed into RNA and processed into multiple guide RNAs to direct TIGR-associated proteins to their DNA targets. science.org/doi/10.1126/sc…

Sequencing by Expansion (SBX) -- a novel, high-throughput single-molecule sequencing technology biorxiv.org/content/10.110… #biorxiv_genomic

Did you know cancer cells can be electrically active, just like neurons? This electrical activity plays a key role in tumor progression. Excited to share my first postdoc paper at @TheCrick (and first paper from @LiGroup_Crick), published in @Nature! nature.com/articles/s4158…

New #preprint from our lab at @IMBA_Vienna ! How do DNA breaks locate homology sites in the vast space of the human genome? We show how #cohesin guides homology search for faithful repair! Read more 👉 tinyurl.com/DSBcohesin Follow along for key insights! 🧵1/12

@binzmit Congratulations!

Our paper is now online at Sci Adv: science.org/doi/10.1126/sc… See also the accompanying news: news.mit.edu/2025/with-gene…

@jmuiuc 新春快乐！！！

May all things go your way in the new year! 新春快乐，万事如意！🎉✨🐍

@XueMande Thank you!

Thrilled to introduce a new single-cell 3D genome analysis tool to our toolkit! Our high-throughput droplet Hi-C (dscHi-C) and dscHi-C-multiome methods use droplet microfluidics to generate tens of thousands of single-cell Hi-C profiles with high sensitivity in a single batch.

@Xieeee4 Thank you, Yang. Your droplet Hi-C method is a significant advancement in the field.

A new droplet-based single-cell Hi-C and multiomic method has just been released! Exciting to see its application on aging mouse brain. Congratulations to the Honggui and others! 🎉

@Michael_Xupb Thank you, Peibo!

@Wu_Honggui Congrats Honggui! that's another powerful tool for the field!

.@LanJiangBoston and colleagues developed droplet-based UDA-seq by incorporating a post-indexing step into two-round combinatorial indexing, enhancing throughput for large-scale single-cell analysis. nature.com/articles/s4159…