Craig C. Mello

@anne_churchland @biorxivpreprint @CSHL @farznaj @GeorgeBekheet @analog_ashley @simonMusall Way to go Anne! BioRxiv publishing is soooo awesome!! Take that reviewer #3! How about a limerick, that captures the feeling?

Celebrating @biorxivpreprint at the Blackford Bar. @CSHL @farznaj @GeorgeBekheet @analog_ashley @simonMusall

@RNA_biologist @JMTali @SpitaleLab Dear Jagannathan lab, Please surprise your PI with some nice GFP positive animals!!!

@JMTali @craigedit @SpitaleLab This sounds cool! We might give it a shot... (unless my lab surprises me with a GFP-tagged cell line when I get back from this conference :D)

NEW CRISPR protocol: biorxiv.org/cgi/content/sh… Please share with your friends and let me know how the hybrid donors work for you! The idea is two PCR products one with homology arms, one without, mix melt and anneal. The hybrids improve efficiency of GFP-size inserts 5-10X

@genes_edinburgh Good question! We have not carefully compared the tox of the hybrids to non-hybrid PCR products. Hybrids are more effective but could have a higher toxicity. Nevertheless they give many more GFP positive animals so its all good.

@craigedit Brilliant work! Just wondering do you see some toxicity with the sticky-ends donor DNA as compared to ssDNA or dsDNA?

One more CRISPR story we just posted on Biorxiv. Adding 5' modifications to donors stimulates HDR and donor potency. Probably does so by preventing end joining reactions that reduce available donor in cells. Please pass along. biorxiv.org/cgi/content/sh…

@pollyp1 @HarmitMalik We have not tried it in mosquitoes, we use OFF instead. Ha ha. I think we have some preliminary data it helps in vertebrate cells. Please let us know if works for you!

Don't forget to optimize your RNP concentration using a co-injection marker! It is toxic, you will still get short edits with ssODNs but you won't get long edits if the conc. is too high. Worms getting long dsDNA seem to be more sensitive to RNP dose.

@LubanLab @MaehrLab @UMassMedical @UMassMedRTI I'm taking notes on my iPhone! Really, I was.

@MaehrLab pours it on for the Program in Molecular Medicine @UMassMedical with a thymic developmental genetics tour-de-force, while a Mello @UMassMedRTI Craig worm guy soaks it all up

@anne_churchland The consensus is that I need some instruction in limerick composition!

@SpitaleLab How did you know? I can't shake this reviewer. No mechanistic insight into the meaning of life or kiteboarding!

@craigedit Must’ve been reviewer #3!!!

REJECTION: "Thank you for sending us the details of your proposed new record category 'longest distance kite boarding in a calendar year'. Unfortunately, after thoroughly reviewing your application, we are afraid to say that we cannot accept your proposal." Guinness World Records

I meant 4098 miles in 2017.

The pew meeting in Tucson was fabulous! The guest speakers reminded us, our society relies on us! The talks were diverse, concise, and inspired! And although the skit was quite dumb, we had lots of fun. We're just bummed that Torsten retired. (Inspired by Anne Churchland)

Just back from the best PEW meeting ever! Thanks to all the scholars, fellows, advisors and the whole PEW team especially Rebecca and Kara. Torsten, thanks for your vision! You will be back! I'm new to twitter so please add me to your contacts. Please stay in touch!!