David Hoffman

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David Hoffman

David Hoffman

@davephoffman

Sr. Staff Optical Engineer at @10xGenomics: 🔬+ 🧬. Views are my own (whose else’s would they be?)

Pleasanton, CA Katılım Ekim 2011
60 Takip Edilen488 Takipçiler
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Michael 英泉 Eisen
Michael 英泉 Eisen@mbeisen·
Here is the letter I submitted in response to the NIH's Request for Information on Maximizing Research Funds by Limiting Allowable Publishing Costs
Michael 英泉 Eisen tweet mediaMichael 英泉 Eisen tweet mediaMichael 英泉 Eisen tweet mediaMichael 英泉 Eisen tweet media
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Eric Betzig
Eric Betzig@Eric_Betzig·
Correlative two color 3D super-resolution single molecule localization microscopy and block face electron microscopy of mitochondria and the endoplasmic reticulum in vitreously frozen COS-7 cells doi.org/10.1126/scienc…
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Ruixuan Gao
Ruixuan Gao@Ruixuan3·
(1/x) We are excited to introduce VIPS (Volumetric Imaging of biological specimens via Photochemical Sectioning) in our preprint! VIPS uses a light-based process called “photochemical sectioning” to achieve petabyte-scale high/super-resolution imaging: biorxiv.org/content/10.110…
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Nature Methods
Nature Methods@naturemethods·
We're very pleased to announce the February issue of Nature Methods, which includes a "mini-Focus" follow-up to our July 2023 Focus Issue on the future of bioimage analysis! (please see thread) nature.com/nmeth/volumes/…
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Jonny Nixon-Abell
Jonny Nixon-Abell@AbellJonny·
✅💡 very happy to have this paper finally out. Chris and I started working on this project in 2017…so great to get this across the line at last! nature.com/articles/s4158…
Jonny Nixon-Abell@AbellJonny

🔬🚨 New preprint from @cjobara and myself during our time together in the @JLS_Lab and Blackstone lab. Motion of single molecular tethers reveals dynamic subdomains at ER-Mito contact sites...a (belated) thread🧵...(1/9)

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Sadaf Shadan
Sadaf Shadan@sshadan·
Cellular organelles exchange molecular information through transient contact sites, which are sensitive to experimental analysis. Reporting @nature, scientists use advanced microscopy to map and analyse dynamic subdomains at ER-mitochondria contact sites. shorturl.at/jAX39
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nature
nature@Nature·
Nature research paper: Motion of VAPB molecules reveals ER–mitochondria contact site subdomains go.nature.com/4bcisy8
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David Cook
David Cook@DavidPCook·
Excited to share our comparison of Xenium and CosMx data! I feel like a lot of labs/core facilities have made similar observations internally, so we're really glad to put this out for the broader community. biorxiv.org/content/10.110… 1/n
Luciano Martelotto 🛠🧬💻🇦🇺@LGMartelotto

I’m going to quietly leave this here…but not without a shoutout to @DavidPCook, @kirkbjensen and @Kellieiswise for their AMAZING work. 🙌 A Comparative Analysis of Imaging-Based Spatial Transcriptomics Platforms biorxiv.org/content/10.110…

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Jennifer Waters
Jennifer Waters@JenCWaters·
If you are imaging more than one fluorophore in your specimen, be sure to perform the correct controls to ensure your results are not confounded by bleedthrough or autofluorescence! youtu.be/D_vxHMWC2mQ
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10x Genomics
10x Genomics@10xGenomics·
What if you could explore 1,000s of your genes at subcellular resolution & get deeper cellular and spatial insights? What if you could do it with near-instant data after a run? Don’t wonder: find out how Xenium can take your ‘what ifs’ to ‘what next’. 10xgen.com/47blS2g
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Adam Bowman
Adam Bowman@AdamB0wman·
Our paper on fluorescence lifetime voltage imaging of neurons is out! We combine kilohertz frame rate, micrometer spatial scale, and picosecond lifetime resolution in vivo. Check out the videos and original thread below. @ChengHuangThu science.org/doi/10.1126/sc…
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Gregory Lab
Gregory Lab@TheGregoryLab·
Image provided by @10xGenomics of a whole mouse pup run using the #Xenium Mouse Tissue Atlassing panel! 🧬👏
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Talley Lambert
Talley Lambert@TalleyJLambert·
The nd2 package (Nikon NIS elements file reader for python) just got a major internal refactor. v0.6.0 reimplements the outputs of the official SDK in pure python. (No more wrapping of c code) Speed is improved, and it’s easier to install anywhere now. github.com/tlambert03/nd2
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Keşfet

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