Karl Kremling

Today, I'm excited to present the second big chunk of my Ph.D. work. We are building a thorough assessment of the burgeoning field of gene expression forecasting. Something is deeply wrong.🧵 - Preprint: biorxiv.org/content/10.110… - Code: #a-systematic-comparison-of-computational-methods-for-expression-forecasting-with-pereggrn" target="_blank" rel="nofollow noopener">github.com/ekernf01/pertu…

Demis -- I think AlphaFold3 is really exciting. As Reviewer #3, I got great results from the server. I tried hard to get @Nature to urge you to release the code but was unsuccessful. I did not get it for re-review so I don't know if you responded. So why no code? @GoogleDeepMind

It pains me to see facile critiques of GWAS on here from our clinical/biostats friends while the many actually good reasons to be critical of GWAS get little attention. So here's a thread on what GWAS does, what critics get wrong, and where GWAS is genuinely still lacking. 🧵:

Are you a comp biologist looking for your next position? Do you dream of nominating loci and seeing those targets edited in planta? Join us @Inari_Ag ! Previous exp. in plants is not required. Feel free to apply below or reach out if you have q's. grnh.se/5a04d3c12us

Important work by @hannesclaeys @Inari_Ag using CRISPR for gene #upregulation in maize through enhancer insertion. Happy to have made a small contribution to the development of this technology. @PSB_VIB onlinelibrary.wiley.com/doi/10.1111/pb…

My article is now out in @Nature! It’s been ~1 year since my post about the flaws within 2 high-profile articles that claimed 33-68% increases in crop yield. Our paper describes common issues & suggests yield-testing criteria for future pubs. A thread🧵 nature.com/articles/d4158…

Join us tmr for the 🌽#Zeavolution 🌽seminar at 11 ET / 8 PT from @Jgdp_S_Sidhu's (Jonathan Lynch lab) - DM for Zoom link! Also, we've had a cancellation for next week's seminar (May 31); feel free to DM me if you'd like to take the last slot of the semester!

@MariaAOrtegaP Thanks, this is a great reference

@hauteHominy I have experience with lyophilized poplar. I did not observe significant differences between freeze-dried and liquid N2 processed samples, for GOIs in qRT-PCRs. Also, pubs.acs.org/doi/full/10.10…

Does anyone have experience with lyophilization before RNA extraction for high-moisture plant tissues that turn rock-hard when frozen in liq N2? Seems like the transcriptome would shift, but the non-plant lit suggests this isn't an issue. Has anyone done it in plants?

@CherrityG @NonoyBandillo Would love to, but this is thousands of samples.

@hauteHominy @NonoyBandillo What tissues? My first recommendation is to always go the old-fashioned mortar + pestle route, then work from there.

Join us for the 🌽#zeavolution seminar tomorrow at 11 EST / 8 PST from Brandon Monier who will talk about work done in the @EdBuckler lab! As always, DM for a zoom link.

Join us for the 🌽🌽 #zeavolution seminar tomorrow at 11 EST / 8 PST from @juliosbernal who will talk about work done in his lab! As always, DM for a zoom link.

Join us for the #zeavolution seminar tomorrow from @JeremyPardo2 who will talk about work done in the @bob_vanburen lab! As always, DM for a zoom link.

Join us tomorrow for the #zeavolution seminar from Shawn Thatcher (of @corteva ) at 11ET / 8PT! Feel free to DM for the Zoom link.

🚨You can now turn off the Chat History in ChatGPT! This means that your conversation is not used to train our models nor saved in the UI. Great move by @OpenAI!

Join us tomorrow morning at 11 ET / 8 PT for Travis Wrightsman's #zeavolution🌽🌽 seminar! As always, feel free to reach out for a link to the zoom meeting

Join us tomorrow (Wed) at 11 ET / 8 PT for 🌽#Zeavolution🌽 seminars from Ruth Epstein and Tom Kosfeld of the Pawlowski and @BaxterTwi labs! As always, DM for zoom link!

Thanks to @vbern for the informative talk at #zeavolution 🌽 today about the invaluable maize resources of the North Central Regional Plant Introduction Station (NCRPIS) at @usda-ARS / @IowaStateU!

Join us at #zeavolution🌽🌽 tomorrow for Eli Rodgers Melnick's (@elirm) talk at 11 ET / 8 PT. As always talks are on zoom and please message me for a link. See you then!

Join us tomorrow at 11ET/8PT for the #Zeavolution talk by Nikita Sajai (@cornellNS623 ), who is in Wojtek Pawlowski's lab. As always, please message me if you'd like the zoom link or access to the slack group. Also, here is the speaker lineup #gid=1081974968" target="_blank" rel="nofollow noopener">docs.google.com/spreadsheets/d…