Joseph Longo

I’m excited to share our preprint, where we describe a critical fate of glucose in physiologically activated CD8+ T cells. @DrRGJonesLab @VAIMetabolism biorxiv.org/content/10.110…

Excited to share our work from the @PospisilikJ lab, now in #NatureCancer! #Epigenetic heterogeneity via Trim28, arising early in #development, shapes long-term #cancer risk. Check it out: nature.com/articles/s4301…

@OwenJ457 @AnnualReviews Congrats Owen!

1/ 🌟 Excited to share our latest review in Annual Review of Pathology: Mechanisms of Disease! @AnnualReviews 🌟 Obesity isn’t just a metabolic disorder—it’s a fundamentally altered immunological state. Read it here: annualreviews.org/content/journa…

Thrilled to share our latest study in @SciImmunology! We reveal the critical role of Srebf2-dependent cholesterol metabolism in the clonal expansion of insulin-sensitizing ST2hi VAT Treg subsets, and how obesity disrupts this process. science.org/doi/10.1126/sc…

Happy to announce our collaboration with @Tcellogic on metabolic features of exhausted T cells is now out in @ScienceMagazine. TL;DR they can't use acetate to fuel their function. Great work by Shixin Ma and Mike Dahabieh. science.org/doi/10.1126/sc…

I am proud to share the @DrRGJonesLab most recent preprint and the bulk of my thesis project. We show that T cells mediate the anti-tumor effects of dietary restriction. @VAInstitute @VAIMetabolism biorxiv.org/content/10.110…

1/ Did you know? Glycans—complex sugar structures—decorate almost every protein processed by the ER/Golgi. These N-glycans coat our cells, playing critical roles in cell interactions and immune processes. 🍬 Curious about how they vary? Let’s dive in!

I've been on a Twitter moratorium for the last week given the.. news, but I'm happy to announce a new story from our group @NatImmunol describing a metabolic sensitivity of exhausted T cells to lactic acid in tumors! 1/ nature.com/articles/s4159…

Today in @CellCellPress we report a new pathway of ketone metabolism. This BHB shunt generates an orphan family of anorexigenic ketone metabolites that are chemical cousins of exercise-inducible Lac-Phe. Congratulations @mariadolores_mg @Stanford_ChEMH cell.com/cell/fulltext/…

@isaac_s_harris Congrats, Isaac!!

New pre-print from the lab! We describe an alternative route for β-hydroxybutyrate metabolism that allows it to bypass the TCA cycle to support fatty acid synthesis in cancer cells. Check it out! 👇 biorxiv.org/content/10.110…

Have you ever wondered how mitochondrial and cytosolic pathways for de novo lipogenesis (DNL) interact? We did! Published Online today (cell.com/cell-metabolis…) and led by first-author @adam_rauckhorst, we traced 13C-labeled lactate/pyruvate, acetate, the ketone acetoacetate, and leucine in several mouse models of disrupted hepatic mitochondrial and cytosolic acetyl-CoA generating pathways. Surprisingly, deleting the mitochondria citrate carrier (CiC) increased DNL from both exogeneous and leucine-generated acetoacetate. Overall, our findings delineate a mitochondrial-cytosolic DNL substrate supply network that may guide more accurate therapeutic development for MASLD and T2D. Many thanks to our expert collaborators: No X Ryan Sheldon, now leading metabolomics at the @VAInstitute, who helped start the project and develop the necessary mass spec methods with @adam_rauckhorst; for @StanislawDeja and @Isotopomer for deuterium tracing by NMR and careful contibutions to quantitatively framing our mass spec data; and Tom Vallim of @TarlingVallim for guidance with in vivo gene deletion by AAV CRISPRs. Please also see the exciting co-published manuscript from @WellenLab and Christian Metallo Labs (cell.com/cell-metabolis…) showing that bempadoic acid does more than just inhibit ACLY! @FOEDRC, @UIowaResearch, @UIMolecPhysBio

🚨 Preprint alert! Our new work uncovering that glutathione (GSH) boosts tumor growth by supplying amino acids is now available at @bioRxiv! Check it out 👇🏼and follow along for the #tweetorial (1/10) biorxiv.org/content/10.110…

Excited to share the first research publication from our lab! Lipid availability influences ferroptosis sensitivity in cancer cells by regulating polyunsaturated fatty acid trafficking sciencedirect.com/science/articl…

Catabolism of extracellular glutathione supplies amino acids to support tumor growth biorxiv.org/content/10.110… And a very exciting piece of work from @isaac_s_harris & team!

Glucose-dependent glycosphingolipid biosynthesis fuels CD8+ T cell function and tumor control biorxiv.org/cgi/content/sh… #biorxiv_immuno

This work was made possible by our outstanding core facilities @VAInstitute and the help of our collaborators. Please pass along any thoughts or feedback you may have!

Taken together, we propose that GSL biosynthesis tethers CD8+ T cell-mediated immune responses to glucose availability, and that targeting GSL metabolism in T cells may be an effective strategy to modulate T cell function.

I’m excited to share our preprint, where we describe a critical fate of glucose in physiologically activated CD8+ T cells. @DrRGJonesLab @VAIMetabolism biorxiv.org/content/10.110…