Kadina Johnston

Happy to share that our work "A combinatorially complete epistatic fitness landscape in an enzyme active site" by myself, @patrickalmhjell, @EllaWD_PhD, Grace Liu, @NJP_Chem, @jsunn_y, and @francesarnold is now out in @PNASNews! (1/15) pnas.org/doi/10.1073/pn…

Protein engineers and biochemists (and everyone, really), check out this awesome work by @kadinaj constructing a 160,000-member fitness landscape in the active site of (my favorite enzyme!) TrpB. There's one neat result (among many) that I wanted to discuss in more detail: (1/9)

Have you ever wondered about how epistasis affects protein engineering efforts? In this case study, we created a combinatorial fitness landscape to explore just that. I am so proud of the work that @kadinaj (and everyone else!) put in to make this project a reality. Check it out!

Great work by @kadinaj and her team--here is a complete enzyme 4-site dataset for #ML4proteins, folks! #enzyme.

Please feel free to reach out to me if you would like a copy of the manuscript! (15/15)

All data and code for this work is publicly available! Raw fastq reads are on the SRA: ncbi.nlm.nih.gov/sra/?term=PRJN…. Processed data and software are available at CaltechData: data.caltech.edu/records/h5rah-…. (14/15)

Happy to share that our work "A combinatorially complete epistatic fitness landscape in an enzyme active site" by myself, @patrickalmhjell, @EllaWD_PhD, Grace Liu, @NJP_Chem, @jsunn_y, and @francesarnold is now out in @PNASNews! (1/15) pnas.org/doi/10.1073/pn…

Excited to finally receive my degree after defending my thesis back in September! I had an amazing ride at @Caltech in the Arnold lab, and @francesarnold taught me so much about doing science and sharing it with others. Thank you to everyone who made my time there so meaningful!

Merck Discovery Biologics is #hiring! Come join us if you are passionate about using cell free expression systems to impact the discovery and engineering of therapeutic #proteins! jobs.merck.com/us/en/job/R294…