Patrick Almhjell

385 posts

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Patrick Almhjell

Patrick Almhjell

@patrickalmhjell

Trying to understand (and enjoy) the little things — postdoc, probing enzymes w/ @herschlab+@fordycelab — PhD, engineering them w/ @francesarnold — he/him

The Sierras Katılım Ekim 2018
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Patrick Almhjell
Patrick Almhjell@patrickalmhjell·
My primary PhD project with @francesarnold at @Caltech is out now in @nchembio! Here we show that the β-subunit of tryptophan synthase (TrpB) is a latent *tyrosine* synthase (TyrS), capable of regioselective and irreversible alkylation of phenols to tyrosines. Read more below:
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Chang Liu
Chang Liu@chang_c_liu·
Our work describing extensive gene divergence on laboratory timescales with upgraded OrthoRep systems is now out in @ScienceMagazine here - science.org/doi/10.1126/sc…. X thread describing the preprint is quoted below. Congratulations to @rix_gordon and team!
Chang Liu@chang_c_liu

In this work, we bring OrthoRep’s mutation rate up to 10^-4 substitutions per base, allowing us to durably hypermutate and evolve chosen genes in vivo at 1-million times the genomic mutation rate of the host yeast cell. What does this afford? biorxiv.org/content/10.110…

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Patrick Almhjell
Patrick Almhjell@patrickalmhjell·
@FloreanMatilde @OConnor_lab This is so cool!! I did the tyrosine synthase evolution work – it’s awesome to see what some of these TrpB-like enzymes actually do. Amazing work! (Fun to see you try the phenol experiment at the end there, but the explanation you found makes a lot more sense for these plants.)
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Jason Yang
Jason Yang@jsunn_y·
Was great to be a part of this project! Kadina put together a great sequence-fitness dataset, and it’ll be particularly useful for testing machine learning methods!
Kadina Johnston@kadinaj

Happy to share that our work "A combinatorially complete epistatic fitness landscape in an enzyme active site" by myself, @patrickalmhjell, @EllaWD_PhD, Grace Liu, @NJP_Chem, @jsunn_y, and @francesarnold is now out in @PNASNews! (1/15) pnas.org/doi/10.1073/pn…

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Patrick Almhjell
Patrick Almhjell@patrickalmhjell·
There is a lot more to dig into in this dataset, but @kadinaj did a great job of working through it to come up with new insights in enzyme evolution and protein engineering. Give it a read! (9/9) x.com/kadinaj/status…
Kadina Johnston@kadinaj

Happy to share that our work "A combinatorially complete epistatic fitness landscape in an enzyme active site" by myself, @patrickalmhjell, @EllaWD_PhD, Grace Liu, @NJP_Chem, @jsunn_y, and @francesarnold is now out in @PNASNews! (1/15) pnas.org/doi/10.1073/pn…

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Patrick Almhjell
Patrick Almhjell@patrickalmhjell·
In other words, did this change in selection pressures unlock access to an entirely new optimum that is relevant under the original, native conditions, or only under these new, slightly altered conditions? My money's on the former, but we'll have to see! (8/9)
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Patrick Almhjell
Patrick Almhjell@patrickalmhjell·
Protein engineers and biochemists (and everyone, really), check out this awesome work by @kadinaj constructing a 160,000-member fitness landscape in the active site of (my favorite enzyme!) TrpB. There's one neat result (among many) that I wanted to discuss in more detail: (1/9)
Kadina Johnston@kadinaj

Happy to share that our work "A combinatorially complete epistatic fitness landscape in an enzyme active site" by myself, @patrickalmhjell, @EllaWD_PhD, Grace Liu, @NJP_Chem, @jsunn_y, and @francesarnold is now out in @PNASNews! (1/15) pnas.org/doi/10.1073/pn…

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Dr. Ella Watkins-Dulaney
Dr. Ella Watkins-Dulaney@EllaWD_PhD·
Have you ever wondered about how epistasis affects protein engineering efforts? In this case study, we created a combinatorial fitness landscape to explore just that. I am so proud of the work that @kadinaj (and everyone else!) put in to make this project a reality. Check it out!
Kadina Johnston@kadinaj

Happy to share that our work "A combinatorially complete epistatic fitness landscape in an enzyme active site" by myself, @patrickalmhjell, @EllaWD_PhD, Grace Liu, @NJP_Chem, @jsunn_y, and @francesarnold is now out in @PNASNews! (1/15) pnas.org/doi/10.1073/pn…

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Anuvab Das
Anuvab Das@anuvab_chem·
I am excited to share our latest discovery which is out now in @J_A_C_S! This study uncovers how engineered hemoproteins can leverage biological nitrogen cycle intermediates for enzymatic C–H amination, opening new paths towards sustainable synthesis. pubs.acs.org/doi/10.1021/ja…
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Frances Arnold
Frances Arnold@francesarnold·
What an honor to be chosen for the Priestley Medal. #Enzymes are the best chemists, of course, but the Priestley medalists are no slouches...and include many of my colleagues @CaltechCCE. Thank you!
ACS Pressroom@ACSpressroom

Congrats to #NobelLaureate @francesarnold, recipient of the 2025 Priestley Medal, the highest honor awarded by ACS which recognizes an individual for distinguished service to #chemistry. brnw.ch/21wLb7x #ChemTwitter #Awards #NobelPrize

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Patrick Almhjell
Patrick Almhjell@patrickalmhjell·
My primary PhD project with @francesarnold at @Caltech is out now in @nchembio! Here we show that the β-subunit of tryptophan synthase (TrpB) is a latent *tyrosine* synthase (TyrS), capable of regioselective and irreversible alkylation of phenols to tyrosines. Read more below:
Patrick Almhjell tweet media
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Keşfet

@ScienceMagazine @rix_gordon @OConnor_lab @kadinaj @EllaWD_PhD @NJP_Chem @jsunn_y @francesarnold