Massimo Onesto

10 posts

Massimo Onesto

Massimo Onesto

@massimo_onesto

PhD Candidate in Pasca Lab @ Stanford University; Working to understand the molecular mechanisms of human axon guidance.

Katılım Mart 2022
50 Takip Edilen68 Takipçiler
Massimo Onesto retweetledi
Sergiu P. Pasca
Sergiu P. Pasca@Sergiu_P_Pasca·
Our latest work is out today in @ScienceMagazine: a self-organizing system that models organizer-driven patterning and commissural axon guidance in human neural development. This was achieved with human midline #assembloids by combining floor plate and spinal cord #organoids from pluripotent stem cells. A key advance is the three-part configuration (a floor plate squeezed in between two spinal cords), which enables human axons to cross the midline (and the subsequent synchronization of activity between the two parts seen in the video below). This platform opens the door to many applications; here, we used it to screen and identify human-enriched midline crossing regulators. Work led by @massimo_onesto with Neal Amin
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Massimo Onesto retweetledi
Kevin Kelley
Kevin Kelley@kevinwkelley·
Great to see our paper online! Amazing collaboration with @neal_amin over the years. With invaluable input from @KaganovskyNeuro, @massimo_onesto, Jin, Yuki, @jamespmcqueen, Noah, and others in @Sergiu_P_Pasca group.
Sergiu P. Pasca@Sergiu_P_Pasca

Today we report in @CellStemCell a systematic approach to generate neural #organoids resembling various domains of the neural axis. @neal_amin & @kevinwkelley developed a multiplexed morphogen screen to generate hundreds of #organoids that ultimately transcriptionally mapped to almost 2/3 of the clusters in the 1st trimester human CNS Also led to generating surprising cell types, including the primate-enriched TAC3 neurons or Purkinje cells of the cerebellum (more on that soon) Check out the paper and summary below

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Sergiu P. Pasca
Sergiu P. Pasca@Sergiu_P_Pasca·
We recently introduced human neural loop #assembloids created from 4 parts to study the cortico-striato-midbrain-thalamo-cortical pathway Here’s a short animated summary of the work led by Yuki Miura and Ji-Il Kim in the lab
GIF
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Massimo Onesto
Massimo Onesto@massimo_onesto·
I’m excited to share that my review article on #assembloids just dropped in Cell Stem Cell! Check out the post below to learn more about this fast-evolving field and its potential for uncovering new insights into human biology and finding cures for diseases.
Sergiu P. Pasca@Sergiu_P_Pasca

Assembloids have emerged as a powerful tool for studying previously inaccessible processes of development and tissue homeostasis. While initial #assembloids were developed to model human brain development, there are now models for most organ systems, from the bladder to the heart and gastrointestinal system. In our new article in @CellStemCell, we summarize recent advances in the generation and application of neural and non-neural assembloids to explore complex cell-cell interactions. Effort led by @massimo_onesto and Ji-Il Kim in the lab

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Sergiu P. Pasca
Sergiu P. Pasca@Sergiu_P_Pasca·
Assembloids have emerged as a powerful tool for studying previously inaccessible processes of development and tissue homeostasis. While initial #assembloids were developed to model human brain development, there are now models for most organ systems, from the bladder to the heart and gastrointestinal system. In our new article in @CellStemCell, we summarize recent advances in the generation and application of neural and non-neural assembloids to explore complex cell-cell interactions. Effort led by @massimo_onesto and Ji-Il Kim in the lab
Sergiu P. Pasca tweet mediaSergiu P. Pasca tweet media
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Sergiu P. Pasca
Sergiu P. Pasca@Sergiu_P_Pasca·
Hard to believe, but our lab at @Stanford celebrates its 10th anniversary this year. It feels like we started just yesterday, yet so much has happened. Here's a quick selfie with this remarkable group of scientists, working with more enthusiasm than ever to unravel the mysteries of the human brain and its disorders. Warmest salutations to the many lab alumni scattered across the world!
Sergiu P. Pasca tweet media
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Xiao Yang
Xiao Yang@XiaoYang63·
Thrilled to share that I will join @JohnsHopkins @JHUBME as an Assistant Professor in 2025! The Yang Lab will develop novel bioelectronics and biomaterials for brain-machine interfaces, regenerative medicine, and the study of human neural development and diseases.
Xiao Yang tweet media
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Massimo Onesto
Massimo Onesto@massimo_onesto·
Excited to share a preview of my thesis work in @Sergiu_P_Pasca's lab at Stanford University! We’ve successfully generated human midline assembloids and identified regulators of midline axon guidance facilitated by the floor plate. Dive into our preprint for all the details!
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Massimo Onesto retweetledi
Sergiu P. Pasca
Sergiu P. Pasca@Sergiu_P_Pasca·
Cells located in the midline of the embryo play crucial roles in development. These cells often influence cell fate decisions and guide axons to establish bilateral connections. Despite their importance, studying these transient cells and their complex interactions has been challenging. Today, we introduce human midline #assembloids—3D self-organizing cellular clusters derived from stem cells by the functional integration of floor plate #organoids and spinal cord #organoids. We demonstrate that midline assembloids enable cell specification and axon guidance in human neurons. Additionally, we use these assembloids to screen and identify human-enriched factors that control these processes. Work led by the incredible @massimo_onesto & @neal_amin!! More details in the preprint 👇
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Keşfet

@ScienceMagazine @neal_amin @KaganovskyNeuro @jamespmcqueen @Sergiu_P_Pasca @CellStemCell @Stanford @JohnsHopkins