Thorsten Wagner

TomoTwin official release pt 1 🥳! After some code streamlining and writing documentation we’ve released the first stable build of TomoTwin. Here’s an example of TomoTwin picking Tc toxins based on a single reference without having to be trained on the protein beforehand. 🧵1/6

Forward-looking preview by @WUBin10069342 to our latest paper @Structure_CP by @AndrewNChang highlighting the power of #AI in #cryoEM of patient-derived samples: tinyurl.com/tu2fyvme Paper: tinyurl.com/3cycffzm Thanks to @pixelbaumarkt @Intein for #crYOLO, & @cryosparc

Sebastian Tacke has been honored with the Paper of the Year Award by the Journal of Structural Biology @ElsevierConnect for his innovative technical developments in the field of #cryoET #cryoEM Congratulations! #teamtomo

One more piece of evidence to add to the pile. This was an extremely heretic viewpoint in early 2023, and now it is increasingly becoming self-evident conventional wisdom.

Really happy to share that the last chapter of my submitted phd thesis is already on biorxiv!🥳 Using a tophat transform, I made template matching more precise, allowing automated annotation with little false positives! #teamtomo biorxiv.org/content/10.110…

We are proud of @oleg_sitsel former @Intein lab postdoc who is now Assistant Professor and head of the Marine Structural Biology Unit @OISTedu . We wish him much success and all the best on beautiful #okinawa #cryoEM

@mpimoph @oleg_sitsel @Intein @OISTedu Oleg, I wish you all the best for your new position. We will miss you, your enthusiasm and your loud laughter!!! Don't get eaten by sharks during sample collection ;-)

Curious about the molecular mechanism of #actin elongation by #formins? Then check out our latest work in @ScienceMagazine! This was possible due to outstanding team effort. Big thanks to co-first author @WoutOosterheert, Bieling lab and @intein @mpimoph science.org/doi/10.1126/sc…

Termine in anderen Monaten als April sind gar nicht erst buchbar. Ist das euer Ernst? Erinnert mich an die Erfahrungen aus der letzten Folge der @LageNation ...

@stadtdortmund Ich möchte einen Reisepass für meine Kinder beantragen. Die Kinder müssen anwesend sein. Ein Mittwoch um 9 Uhr ist der einzige freie Termin im ganzen April. Meine Kinder sind zu dieser Zeit in der Schule. Soll ich sie etwa beurlauben?

So proud that our Tc toxin release story with @Intein was featured on the cover of today's @NatureMicrobiol issue! 🎉 Check out this 💥explosive 💥tale here: nature.com/articles/s4156…

Are you ready to meet the #toxin with the most AWESOME name? Say hello🤗 to The Makes Caterpillars Floppy 1 toxin! It took us nearly a decade of work🔬 to find out what it looks like, but it was really worth it. Look at this 330 kDa giant beauty full of secrets! Thread 1/8.

My lab has exciting openings for postdocs/staff scientists and a technician. Involves super-cool projects, a dynamic international environment, cutting-edge infrastructure, and a stunningly beautiful tropical island! oist.jp/careers/two-po… oist.jp/careers/resear… Please repost!

It is my great pleasure to announce that I am joining @OISTedu as faculty! My lab will focus on characterizing our oceans’ amazing inhabitants mainly by cryo-electron #tomography, with #corals representing our chief interest. Collaborators always welcome – please reach out to me!

Our paper revealing how specialized #Yersinia cells 🦠release a deadly Tc toxin-containing cocktail ☣️ by blowing themselves up 🧨🤯 is finally out! Read it in @naturemicrobiol: nature.com/articles/s4156… #toxin #pathogen #tomography #explosion #T10SS

Read how a few soldier cells armed to the teeth confer virulence to an entire bacterial #Yersinia population by sacrificing themselves - find the corresponding paper in @naturemicrobiol @Intein @oleg_sitsel @Zhexin_Wang @pixelbaumarkt @maxplanckpress mpi-dortmund.mpg.de/news/soldier-c…

Very excited to share our preprint on the multi-state kinetics of the syringe-like injection mechanism of Tc toxins. Great collaboration between the Raunser @Intein @mpimoph, Seidel @HHU_de, @enricabordignon @sciences_UNIGE and Dong @harvardmed labs. biorxiv.org/content/10.110… 1/3

Great example (thx @proteincapsid for the tomogram) to experience how TomoTwin organizes the embedding space. The virus center is located in a completely different region than the "envelope" :-) Optimized clustering workflow will be released soon tomotwin-cryoet.readthedocs.io/en/stable/

Big thanks to (twitterless) Vicky Trinkaus, @Intein and all our speakers for a stunning 2-day cryoEM/ET winter school @mpimoph - well timed to the first snowfall in Dortmund!

TomoTwin 0.7 is out and ready to use for #TeamTomo :-) This release adds new tools for advanced strategies that describe how to speed up the calculation of embeddings, potentially improve their umaps, and how to refine references/targets! 🧵 All changes: github.com/MPI-Dortmund/t…

The second strategy creates masks using an estimate of the average background embedding. It can exclude large portions of irrelevant regions, dramatically speeding up the embedding step and allowing the umap to focus on relevant embeddings. #strategy-2-speedup-and-improvement-of-embeddings-using-median-filtering" target="_blank" rel="nofollow noopener">tomotwin-cryoet.readthedocs.io/en/stable/stra…

The first strategy helps you to refine manually selected references or cluster targets using umaps. #strategy-1-refinement-of-references-targets-using-umaps" target="_blank" rel="nofollow noopener">tomotwin-cryoet.readthedocs.io/en/stable/stra…