Tushar Kamath

Spatial genomics has existed for many years, but it has often been limited by complex imaging systems, specialized equipment, and $$$. With IRISeq, we wanted to simplify this to a simple PCR rxn. nature.com/articles/s4159…

@SeeFisch Its not clear to me if this is the correct strategy if the etiology of ascites is purely right sided HF and not cirrhosis. Also, the 2.5:1 ratio of mra:loop diuretic is totally made up afaik. Titrating to optimal lytes/volume status as opposed to fixd ratio is likely superior

One of the BIGGEST sources of confusion of diuretic therapy with ascites is getting the dosing wrong. For CHF spironolactone is 25 mg. In Ascites we START at 100 mg and go to maximum of 400 a day. Furosemide is added with 40 mg and maximum 160 mg

2/8 Introducing Synapse-seq: our AAV-based tool for mapping brainwide presynaptic projections or postsynaptic distributions of molecularly-defined neurons, via protein-guided RNA barcoding & single-cell/spatial readouts: doi.org/10.64898/2026.…

@emmakurz @MSKCancerCenter @MGHMedicine @MSK_DeptOfMed Ayyy!! Lfg

Thrilled to have matched into oncology fellowship at @MSKCancerCenter ! (w/ my amazing colleagues and friends @MGHMedicine PSP). I’m truly grateful and excited for this next chapter at @MSK_DeptOfMed, an institution that has served so many I love. Can’t wait to get started 🤍

We are thrilled to have the following superstars join our fellowship program in July 2026.  Congratulations to Erik, Wes, Tushar, Brian, May and Shawna, and congratulations to the MGH GI Division for recruiting such talent.  Welcome to the family!

A truly impressive synthesis of bio-psycho-social!

The first piece of work from my PhD has now been uploaded to biorXiv! Short 🧵below:

Excited to share our preprint, describing a method for heritability partitioning with GWAS sumstats that significantly improves upon S-LDSC Led by the fantastic Hui Li, and co-supervised by @XihongLin #ASHG24 poster 4089F medrxiv.org/content/10.110…

A particularly exciting way to look at transcriptional data using lessons learned from statistical genetics!

How do genetic perturbations change cells? How are these effects shaped by cell type and dosage? How do we best extract insight from modern massive perturbation atlases? Im pleased to share a new preprint where we develop a suite of statistical approaches to these Qs (link below)

Exciting stuff from @LaylaSiraj @julirsch and friends!

@LNuzhna What exactly is a “human aging dz”? Is cancer a human aging dz in that most cancer incidence rates are an exponential function of age? If so, I cant imagine diffuse mets are an act of bodily compensation

If you stare at any human aging disease long enough, you start to notice that it is usually not a “damage” in itself but rather an imperfect attempt to fix damage. And all that the current disease drugs target is this process of biology fixing itself / compensating. I wonder if we’d have a completely different set of diseases to fix once we get really good with halting the process of compensation as we currently know it.

What a fantastic presenter at SCGD!

@CalebLareau @tsion_abay @MerilTakizawa @ChaligneRonan @Satpathology Nice re: quality! Unclear, whether they had RNA (we didn't do any staining on top of just a standard electron micro prep). Likely some organelles that glommed onto the nucleus, maybe rRNA? It may not be a huge issue if you're using the same protocol to compare btwn conditions

@tkam80 @tsion_abay @MerilTakizawa @ChaligneRonan @Satpathology Sorted vs unsorted look as good as Flex by itself. There's no free lunch in multi-omics, but we think we're dangerously close (NYC $0.99 pizza close). we didn't examine the populations except flow and scRNA-- did the extranuc have RNAs? we can look for it in data

What a PERFFect day to share our new preprint! With @tsion_abay #BobStickels @MerilTakizawa @ChaligneRonan and @Satpathology, we introduce PERFF-seq, a new experimental approach to studying rare cells with scRNA-seq via transcript-specific enrichment. biorxiv.org/content/10.110… 1/n

@CalebLareau @tsion_abay @MerilTakizawa @ChaligneRonan @Satpathology Also this would defo be something i wouldve used extensively in grad school, kudos!

@CalebLareau @tsion_abay @MerilTakizawa @ChaligneRonan @Satpathology Cool stuff!! How does the quality of the data compare between the sorted/unsorted? Also, curious if you ever did some EM on the gated v ungated, we did a quick single replicate for our DA neurons w the TF enrichment and found, by eye, there was some extranuc material riding along

@tangming2005 Curious what the alignment quality is for those mt- and rps/rpl reads. In brain data i sparingly trust these populations. Obvi would require stains or flow data to make me really believe it!

single-cell folks, cluster5,6 are CD8T cells, the rest are CD4 T cells. cluster 0 has high ribosomal genes (rapid protein production?) and cluster 2 has high mitochondrial genes (dying?). what's the property of those cells?

@Abdul_Squared @MGHMedicine Thanks man!

@tkam80 @MGHMedicine Big congrats Dr Kamath!!!

Thrilled to have matched at @MGHMedicine in the Stanbury PSTP! Thanks to all my mentors, family, and friends for the support and love. Thank you to all the patients who have taught me so much so far and the ones that I will learn from on my next journey! Doctorin’ soon! 🩺🫡

@dolanmjg @MGHMedicine Thanks Mike!

@tkam80 @MGHMedicine Congratulations Tushar!!!