Bacterial Genetics and Evolution Lab

Si te gusta la Genética Bacteriana, eres residente en Andalucía, menor de 30, licenciad@ en Biología/Biotecnología, con inglés B2 y estás desemplead@, considera solicitar el contrato de 2 años PEJUS7-B1-54 para unirte a nuestro laboratorio en @unisevilla: investigacion.us.es/recursos-human…

@I_Rubio_Somoza I'd try using a tightly regulated promoter (PLTetO-1, for instance), and a DH5alpha strain constituvely overproducing its repressor (in this case TetR). If the repressor is encoded just in the same plasmid, some leakage can occur while the repressor is being expressed.

We synthesize a gene sequence and when we try to amplify the original plasmid in Top10, DH5 alpha or DH10 beta we always end up with a transposon insertion in the miniprep, any tips on how to avoid it?, thanks a lot in advance!

@AaronDelOlmo Otra cuestión también sería por qué se siguen haciendo tests de IQ, cuando hace mucho que se sabe que miden la capacidad para resolver un tipo muy concreto de desafíos, que están sesgados culturalmente y que no suponen una medición científica fiable de inteligencia general.

Gloriosa maestra decide decirle a una madre a los 6 años que su hija es "tonta", que no "da para más" a raíz del WISC. También se lo dice constantemente a la niña. La niña repite tercero de primaria por "tonta". Hoy con 12 años, no sé el motivo, la niña piensa que es tonta.

@salonium Accepting the hypothesis of accidental discovery of penicillin would also imply that Fleming did not know the work of Ernest Duchesne on bacterial inhibition by fungal species, published decades earlier.

I’m a disbeliever in accidental discoveries (at least, in biology). Whenever I’ve looked into one, the story turns out to be false. The most famous is penicillin – supposedly, the fungi wafted in through a window, fell into a petri dish of cultured staphylococci, and suppressed the bacteria’s growth. But in a recent article (asimov.press/p/penicillin-m…), @kevinsblake explains that doesn’t really work (grown staphylococci aren’t affected by penicillin; it only works if introduced before the bacteria begin growing); plus, Fleming’s notes on the discovery provide very little detail and the specific results he described couldn’t be replicated by other scientists (even though penicillin does work against staphylococci when introduced correctly.) There are more: Pasteur’s supposedly accidental discovery of a chicken cholera vaccine was more likely the result of systematic work by his then-assistant, Émile Roux. (jstor.org/stable/2332836…) And, as @NikoMcCarty writes, the discovery of GFP, nanopore sequencing, and optogenetics are also often described as accidents, but none of them happened that way either. nikomc.com/2026/04/01/opt… People love serendipity, so why am I bursting their bubble? I don’t think this is limited to accidental discoveries; I think many historical science anecdotes are highly embellished: - Edward Jenner didn’t deliberately expose a young boy with full-blown smallpox to test his vaccine (he used variolation); and he wasn’t the first to try using cowpox bsky.app/profile/scient… - Cobra catching bounties in British India didn’t lead to a rise in the number of snakebites, and there was only hearsay evidence that cobras were bred in response at all twitter-thread.com/t/169650089580… - Barry Marshall didn’t develop stomach ulcers from drinking a concoction of H. pylori (he did develop gastritis though…) cdn.centerforinquiry.org/wp-content/upl… - No one knows who actually found the highly-productive strain of penicillin on a cantaloupe, but it probably wasn’t 'Moldy Mary' scientificdiscoveries.ars.usda.gov/tellus/stories… But in this case it irks me for an additional reason – it gives the impression that innovation happens sporadically, by chance, when there are actually ways that we can systematically speed it up – such as better funding, institutions and incentives. So: are there any true accidental discoveries that hold up to scrutiny?

@Uruguay @RonaldAraujo_4 @fedeevalverde Andá a explicar cómo de un país de tres millones de habitantes sale un científico reconocido por la revista Nature (N°1) como un Top 10 en el Mundo: @gonzamoratorio en google.com/amp/s/www.bbc.… ...O en 2024 como uno de los 16 líderes mundiales elegido por la Universidad de Yale USA

@pastorams @EnriqueViguera Perfecto, ya sólo faltaría estipular que toda investigación financiada con fondos públicos se publique en plataformas de este tipo o revistas "non-profit", y muchos de los incentivos perniciosos del sistema de publicación científica quedarían anulados. Sentémonos a esperar...

Notición! A la práctica significa que cualquier grupo de investigación afiliado a una institución española podrá enviar sus artículos a #ORE para que sean publicados allí, sin coste, si pasan la revisión por pares abierta. Esto será operativo a partir de este otoño🚀

@joseramosvivas Totalmente de acuerdo. Y aparte, no es exactamente lo mismo que hicieron cuando "crearon la primera bacteria sintética"?

😱😱¿Ciencia ficción o realidad? 🧪🧟‍♂️ ¡Científicos han logrado "revivir" bacterias muertas 🦠✝️! ​Un nuevo estudio del J. Craig Venter Institute marca un hito en la biología sintética. No es solo edición genética, es "reanimación" celular. ​🧵 ↘️ ​1️⃣ Células "Zombie": Los investigadores mataron bacterias (M. capricolum) desactivando su ADN con químicos, dejando solo la estructura celular intacta 🧫. 2️⃣ Trasplante Total: Insertaron un genoma sintético completo en estas bacterias muertas 🧬. 3️⃣ El Despertar: Al recibir el nuevo ADN, la bacteria "muerta" volvió a la vida, empezó a crecer y a dividirse como una especie nueva ⚡. 4️⃣ Sin Antibióticos: A diferencia de métodos antiguos, este sistema no necesita marcadores de selección, lo que lo hace mucho más eficiente para crear organismos a la carta 🛠️. ​Esto abre la puerta, por ejemplo, a crear microbios diseñados para producir combustibles limpios o medicamentos específicos. 🌍💊 ​🔗 @SEMicrobiologia @COSCEorg @ANIH_1 @microBIOblog ​#BiologíaSintética #Ciencia #Genética #Innovación #CraigVenter #Biotecnología #Microbiología 👇 biorxiv.org/content/10.648…

“What Zohran Mamdani can teach us about changing the publishing system.” timeshighereducation.com/opinion/what-z…

@Miriam_Junne No sólo toleró y permitió. El gobierno israelí financió Hamas, como han reconocido sus propios cargos políticos...

Decir que los palestinos sufren ‘por Hamas’ es intentar borrar hechos documentados: Hamas no surgió de la nada. En los 80 Israel toleró y permitió el crecimiento de redes islamistas en Gaza para debilitar a la OLP de Arafat, que era la principal fuerza nacional palestina. Gaza es uno de los territorios más pequeños ¿Nos puedes decir cuánto mide? Ese pequeño territorio es de los más vigilados del planeta. ¿Me estás diciendo que Israel ha demostrado que tiene armamento desarrollado para matar quirúrgicamente líderes en Irán o en Siria y Líbano pero no en Gaza?, ¿cómo te explicas que en Gaza la ‘solución’ haya sido arrasar barrios enteros, hospitales, mezquitas y universidades, “han tenido” que matar decenas de miles de civiles periodistas, rescatistas, doctores, niños, mujeres, refugiados, personal de la ONU buscando a sus enemigos en un territorio que es más pequeño que la isla de Cozumel ? ¿Que deliberadamente les dejan sin agua y sin alimento y abusan de ellos en las cárceles porque es “culpa de Hamas”. Esa excusa para justificar toda su maldad expiró hace tiempo. Ya hay demasiada documentación como para decir que no perdonamos que hayan hecho volver el nazismo en toda su extensión.

@coliescheri @IsraelMFA "Unprovoked". Is this a parody account?

@IsraelMFA Please share more images its so beautiful to watch

Iranian proxy Hezbollah launches unprovoked rocket attacks from Lebanon. Israeli civilians are the target. This is the reality now in northern Israel.

@glopllu69 @FigaredoJoseM Por no hablar de que las reservas de uranio se están agotando...

@FigaredoJoseM El precio medio de hoy en Francia, con nuclear, es de 0.194 euros el kWh, en España, el precio medio es de 0.1609 euros el kWh. Pues no sé si eso de arruinarnos tiene que ver con ésto.

¡Nos quieren arruinados! El daño que este gobierno está haciendo al futuro de España es terrorífico.

¿Os acordáis cuando en el colegio nos ponían discursos de Hitler y nos llevábamos las manos a la cabeza preguntándonos cómo podía haber normalizado la gente las barbaridades que decía? Pues sospecho que de dentro de 80 años los niños se preguntarán lo mismo cuando vean esto.

@MicroBioMol In experimental sciences, ideas get you max 50% of the way; the other 50% relies on their experimental confirmation/refutation. Great ideas "validated" by sloppy experiments that turn out to be wrong slow down scientific progress way more than peer-review does.

"Papers are judged less by the originality of the idea and more by the volume of data, the sophistication of statistics, and the beauty of the figures. Science risks becoming data-rich but idea-poor." Ideas, fascinating and great ideas, are the true motor of scientific progress

@PROMAKOS_ Aparte, Pinker también olvida (deliberadamente, en mi opinión) que esa supuesta cultura occidental basada en los principios de la Ilustración sería incompatible con comportamientos claramente definitorios de dicha cultura, como el colonialismo.

La Ilustración no inventó nada, simplemente secularizó conceptos con arraigo en la civilización del occidente medieval, que es la única civilización occidental real que ha habido (después se dividió en varios proyectos civilizatorios). El problema es que Pinker escribe desde una tradición intelectual angloparlante y protestante que tiene un punto ciego estructural respecto a la cristiandad medieval y católica. En ese canon, la historia de Occidente arranca más o menos con la Reforma, salta a Newton y Locke, y llega a la Revolución Francesa. Lo que ocurrió entre Agustín y Erasmo es oscurantismo o decorado. Las universidades medievales, el derecho canónico como primer sistema jurídico transnacional, la Escolástica como metodología racional antes que cualquier empirismo moderno, la Escuela de Salamanca... todo eso no existe o es anecdótico. No es solo ignorancia, es una narrativa con una función comcreta: si la libertad empieza en la Reforma y la Ilustración, la herencia católica queda fuera del relato fundacional de Occidente. Pinker está describiendo SU versión de occidente.

@thecyrusjanssen Perhaps is not a matter of who is in charge. Under the command of any of its leaders, since WWII the US has been the greatest source of geopolitical instability for the sake of profit.

It's time to say the quiet part out loud: Xi Jinping is a more stable, reliable leader in the world today than Donald Trump Now before the China haters come back with the classic rhetoric: "How much does China pay you to post this?" Just honestly answer these questions: - Which leader has been the biggest source of global instability over the past 12 months? - Which leader launched a global trade war only to have tariffs been ruled unconstitutional by his own government? - Which leader bombed a sovereign nation, kidnapped their leader with the sole purpose of stealing that nation's oil? - Which leader promised his supporters to NEVER start a new war in the Middle East only to lie and march the world closer to WWIII? - Which leader bombed a school killing 165 innocent children only to lie about it and cover up his mistake? - Which leader has destroyed his nation's standing in the world more in the past 12 months than any leader before him? Facts matter and the only logical conclusion is the United States reputation has never been more compromised and destroyed by a single person in history than Donald Trump.

@HaizamAmirah Esto no va se saber o darse cuenta de nada, va de intereses, influencias y oportunidad. Está intentando rebajar su apuesta anterior porque ve que podría perderla, y poder decir que apostaba al caballo ganador sea cual sea.

🇩🇪 El canciller Merz está despertando. Después de apoyar un genocidio en Gaza, agradecer a Israel por hacer el “trabajo sucio” y mostrarse servil en la Casa Blanca, hoy declara: “El colapso de Irán o una guerra por delegación podría tener graves consecuencias para Europa, incluida la seguridad energética y la migración”. ¿Ahora se da cuenta? ¿No tiene asesores sensatos? reuters.com/world/middle-e…

@kevinvdahlgren André Antunes is great, yeah.

This is called Karen core and it’s the best thing I’ve seen in a while.

I don't even know what to write about this one. What am I supposed to say? "Hey everybody, they're lying to us about this war"? Everyone already knows that. Even the people who support this war know all the justifications for it are lies. They know Iran isn't building nukes. They know Iran poses no threat to the United States. They know all that bullshit about Iran cutting out women's wombs and murdering tens of thousands of protesters was evidence-free atrocity propaganda. Nobody needs me to tell them these things. Nobody needs me to tell them that this war is going to kill a whole lot of innocent people and inflict unfathomable amounts of suffering upon our species, both directly during these attacks and indirectly in the chaos and instability ensuing thereafter. Everyone already knows this. Everyone already knows this, and it's happening anyway. They're just doing whatever evil things they want to do, without the slightest regard for public opinion or consent. They're just going right ahead with a military operation to topple Tehran, after decades of inertia for fear of the horrific consequences it would unleash. They're just choking off Cuba using siege warfare, which previous presidents refused to do because it would be a monstrous act of war. They just kidnapped the president of a sovereign nation, which previous administrations had refused to do because it's plainly against international law. They just helped Israel turn Gaza into a gravel parking lot and are now building a giant dystopian tech surveillance encampment to imprison the survivors. They just designated an American company a "supply chain risk to national security" for the first time ever because the AI firm Anthropic refused to let the Pentagon use its technology to operate autonomous killing machines and surveil American citizens — an open admission that the Pentagon plans on using AI to run autonomous killing machines and surveil American citizens. There's an old Frank Zappa quote that's been popping into my head more and more lately: "The illusion of freedom will continue as long as it's profitable to continue the illusion. At the point where the illusion becomes too expensive to maintain, they will just take down the scenery, they will pull back the curtains, they will move the tables and chairs out of the way and you will see the brick wall at the back of the theater." We're seeing a lot more bricks lately. That's all I can think to say right now.

Qué magnífico artículo de Javier Sampedro ⁦@padreoms⁩ !! Gracias por decirlo tan bien y tan claro. La ciencia es intrínsecamente lenta y su motor la curiosidad

@soigomaa People who seek knowledge to understand/help others often are really aiming to understand/help themselves. Most of them never manage to do either. Their solutions most likely are not wisdom; not even useful. When struggling, the only solution is a good professional and hard work.

The man who wrote "How to Save Your Marriage" in the U.S. shot his wife and posted the photo online. Dale Carnegie, author of "How to Win Friends and Influence People," died completely alone. Benjamin Spock, who shaped modern parenting and sold millions of books, had sons who tried to place him in a nursing home. Maria Montessori, the world's most celebrated educator, gave her own son to foster care to raise other people's children. A Korean author behind the bestseller "How to Be Happy" took her own life after years of depression. The pattern is disturbing: the people selling life answers often couldn't save themselves. Coaches. Gurus. Influencers. They package clarity, confidence, and control while privately unraveling. Teaching wisdom doesn't equal living it - and sometimes, the louder the advice, the deeper the chaos behind it. Self-help isn't proof of mastery. It's often proof of searching.