AscidianBioCell

Nice one Benoit

.@AbbyKindberg, @Bush_lab @UCSF and colleagues find that EPH/EPHRIN signaling drives cellular organization by modulating cell contact strength by regulating actomyosin contractility at heterotypic contacts. bit.ly/3ds85t3 #CellSignaling #Cytoskeleton #Development

@CampasLab but would it be also possible to put oil droplets between cells ?

@AscidianBioCell We do use fluorocarbon oils that are biocompatible and do not mix with lipids or water. Also, we use fluorinated surfactants to stabilize the droplet and prevent non-specific interactions at the surface. It seems that you want to use it inside blastomeres... is this correct?

We will talk about your tool in our lab and will probably contact you... does the oil droplet need to be fluorescent ? (no problemo)

@CampasLab yes, that is what we do usually. we would like to confirm forces felt by cells and assess whether there can be inhomogeneity in tension between cells or withun cells (as there is strong segregation of cytoplasm in our cells).

@CampasLab We've been injecting sigma oil or wesson vegetable oil but never tried other types of oil

@AscidianBioCell Great! It really helps a lot if it is fluorescent, specially for 3D reconstructions. However, you can measure stresses with droplets in some cases with only a 2D section and no fluorescence - you just need to see and measure the location of the droplet contour.

Are you ready for our next round of #MBLMarchMadness? We have two great match-ups for you today! From the Deuterostome Division: It's Axolotl v. Sea Squirt and in the Ecdysozoan Division: Beach Hooper v Lobster! Vote in the polls below to choose your winner!

Now Angelica MIGLIOLI from Villefranche sur Mer presents us at #JAP the larval development of Mytilus

I love the idea of shared talks between scientists from different disciplines who work together for #interdisciplinary research. I hope it will show that I hardly know or understand any physics 😩 but that we have learned to discuss through a common language to bridge disciplines

I wouldn't be mad at mask security 😂

Quorangtine

Researchers at @Toliclab optogenetically controlled the important cell-division protein PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces elifesciences.org/articles/61170…

It has been a long week... happy #fluorescencefriday

@lionlchristiaen @RacioppiClaudia @StolfiAlberto yes !! not sure who is the original :-)

Phallusia magic : the pigmented cell leaving the neural epithelium to go into the brain cavity is called the otolith. the other pigmented cell (in green too) is the ocellus. My question : is the otolith undrgoing "cell slithering" ??

@sha_gandhi @BronnerLab @Dev_journal what is your email adress ? mine is : remi.dumollard@obs-vlfr.fr . it would be great to get hold of your plasmid

@AscidianBioCell @BronnerLab @Dev_journal Could you please send me an email? I will be happy to ship the plasmids to your lab while we wait for them to be available through Addgene.

Very pleased to share my most recent work from the @BronnerLab now out (for early access) in @Dev_journal! Our modified #CRISPR vector allows concomitant delivery of Cas9, gRNAs, and a fluorescent protein in transfected cells. dev.biologists.org/content/early/…