Asif Ali

Organelles harbour pH gradients biorxiv.org/content/10.648… #biorxiv_cellbio

@Naam_kafi_hai Asif

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🔬❄️ The new Krios 5 from ⁦@thermosciEMSpec⁩ is looking sharp in its new Midwest Center for Cryo-ET lab ⁦@UWBiochem⁩ @UWMadisonCALS⁩ ⁦@UWMadison⁩! Increasing #cryoEM #teamtomo access for bioscience researchers with support from ⁦@NIH_CommonFund⁩ ❄️🔬

What a terrific recognition of our research field! Dirk Görlich (Max Planck Institute Göttingen) & Steven McKnight (UT Southwestern) received the Lasker Award for their work on low-complexity domains & their phase separation. Read the perspective in PNAS: pnas.org/doi/epdf/10.10…

Excited to share our new work "Dissecting the Cdc37 Co-chaperone Code: Functional Roles in Chaperone-Mediated Stress Adaptation" in press at the Journal of Biological Chemistry jbc.org/article/S0021-… 🔥🔥🔥

Happy to share our community note published in Nature Communications. In this 14-page article (34 authors from 9 countries), we discuss current practices in biomolecular phase separation and hope it will be useful to the biomolecular condensate community. nature.com/articles/s4146…

MOSAIC lattice light sheet xy projection over 2+ hrs in human retinal pigment epithelial cells of endoplasmic reticulum remodeling (cyan) and transport of vesicles (yellow) containing β4-galactosyltransferase....

@slavov_n This is amazing. And during stress when there is sudden halt of rRNA production triggers chaperone stirred condensation of orphan ribosomal proteins. Cells has planB ( preservation model) in stress situation. Here is the link of the study doi.org/10.1038/s41556…

Transcriptional over-expression of genes coding for ribosomal proteins does not elevate protein levels. Protein levels remain stable because the excess subunits are degraded. Inhibition of the proteasome leads to the accumulation of insoluble aggregates.

@declan_c9 This is exciting! It will be interesting to test if it’s reversible after stress and to explore the role of Sis1/Hsp70 in the process.

@Asifcellbio Great piece! We just had a paper out where we found Tpk3 (a PKA subunit in yeast) forms foci during stress that relocalise to nuclear ring structures in the absence of Hsp42. Perhaps this works by a similar mechanism. doi.org/10.1016/j.celr…

Hey #condensate enthusiasts, We just wrote a perspective piece describing biomolecular condensation as a preservation mechanism for orphan proteins during environmental changes. Preserve or destroy: Orphan protein proteostasis and the heat shock response rupress.org/jcb/article/22…

☕️In this issue: A Comment on #VolumeElectronMicroscopy, standards in sample preparation and image acquisition for enhanced reproducibility and data reuse. 👉🏼rdcu.be/dGMhZ nature.com/articles/s4155…

Design principles to tailor Hsp104 therapeutics biorxiv.org/content/10.110…

Our recent work (led by @sammykeyport) on evolution of biomolecular condensation is now highlighted as a featured article (and is open access) 🔬 nature.com/collections/bb… nature.com/articles/s4146…

Online Now: Nucleolar stress caused by arginine-rich peptides triggers a ribosomopathy and accelerates aging in mice dlvr.it/T4cJ7k

Macromolecular condensation organizes nucleolar sub-phases to set up a pH gradient: Cell cell.com/cell/fulltext/…

PRC2 is reported to bind RNA. Yet, genetic experiments have raised serious questions about their functional relevance. Our paper revisits the biochemical evidence supporting widespread PRC2-RNA interactions and finds that many of these do not occur in vivo. tinyurl.com/mr3thkyx

Need a quiet space to read, relax or work? Visit the Arley D. Cathey Learning Center in this "study with me" style video. High-vaulted stone ceilings bring a sense of grandeur to your study session, with a carefully curated mix of classical music. youtu.be/qupg8_2vFgc

amazing integrative study of coronavirus NL63, with cryoET, sub-tomogram classification and averaging (@chiulab, @SLAClab), glycan profiling by mass spec (@DocGatorDawg1), MD (@abhisekhsingha1), and functional infectivity assays (@JingJin10601360) rdcu.be/dqvKa

We are on Cover, #Chaperone #Condensates. Framing Heat Shock response as Condensostasis rather than Proteotoxic.

👋Our November issue's live! Cover: #chaperone #condensates 👉🏿Read: #MitochondrialMetabolism #neuron #mechanotransduction #PhaseSeparation #lipidhomeostasis #ribotoxic stress #genomestability #senescence #MitochondriaTransfer #DEI... & more! nature.com/ncb/volumes/25…