Fulka Lab

Can cycloheximide cause DNA damage? Possibly. Inhibiting protein synthesis stresses cells and raises DNA‑damage markers like γH2AX — but are these actual DNA breaks or is it just induction of markers? #CellBiology #DNA

When you can't replicate published results, self-doubt sets in: why isn't it working? What´s wrong? In desperation, you start optimizing with what you have. Glad we did! We learned a lot, and now we´re looking forward to the key experiment. Exciting! #CellBiology #OocyteResearch

@euvlook Well, the biggest nightmare so far was to try to replicate some of the published data. No idea if we are not doing it right or what (maybe a diffent mouse, maybe different culture...).

@FulkaLab Nice shot，How far are these DNA damages from going out of control? After all, DNA damage is everywhere. Also, what nightmare did you encounter?

How do oocytes handle DNA damage when transcription is shut down? What I thought would be a small, fun project has ballooned into a nightmare of endless variables. Science really isn’t all sunshine and rainbows. #CellBiology #ReproductiveBiology #futureART

Bovine oocytes and embryos closely mirror human ones and are vital to reproductive research, yet they are notoriously challenging to handle. Many thanks to our colleagues in the Department of Reproduction at the Institute of Animal Science. #ReproductiveScience #AnimalScience

We are thrilled to be part of the ART FOR THE FUTURE project, aiming to explore new frontiers, from AI and germ cells in vitro to ethical or legal issues, bridging basic science and clinical practice. More: buff.ly/jF24A2d #futureART #Strategie_AV21 #ReproductiveMedicine

Check out these beauties! Our advanced-maternal-age oocyte media has produced its first embryos. It's both exciting and nerve-wracking, but I love every moment! 🤩 #Embryology #Innovation

How can we tackle issues like DNA damage or mitochondrial dysfunction in embryos? By intervening before the first embryonic replication, we can prevent these issues from becoming permanent, ensuring healthy development. Read more: buff.ly/gPwRBk1 #Genetics #Embryology

@zaffagg3 @Bims_BiomedNews PhD forever? 😁

@FulkaLab @Bims_BiomedNews Certainly not a project you give to a single PhD student 😄

Happy Sunday 🥚! Today we have a spectacular issue of #cebooc featuring a fundamental paper in reprdocutive biology: biomed.news/bims-cebooc/20… Can we clone a mammal indefinitely? Check it out here: pubmed.ncbi.nlm.nih.gov/41876466/ Powered by @Bims_BiomedNews

@TakashiAkeraLab Super-excited to hear your talk!

Thanks a lot for the invitation! Looking forward seeing you soon!!

Curious about why some genes are favored in the egg during meiosis or how selfish genetic elements break Mendel's rules? Join us on April 2nd at the Institute of Experimental Medicine to learn more! #Genetics #Meiosis #ScienceEvent

Ever wondered about the function of nucleoli in zygotes and early embryos, and their interaction with the embryonic genome? Mark your calendars! #Embryology #Genomics

It took me a while to realize that signs of oocyte aging aren't uniform (why would they be, right?): Two mouse strains, similar in age, completely different spindle traits. How much does this apply to human individuals? #OocyteAging #GeneticDiversity

@BoianiMichele Congratulations! Nice paper!

After sharing the advanced publication access few weeks ago, I am now pleased to share the final typeset version, open access, of my group’s latest contribution in Molecular Human Reproduction doi.org/10.1093/molehr… In a nutshell, we report that the first two blastomeres are reciprocally different in terms of epiblast production, for reasons that seem to depend on the site where the spermatozoon penetrated the oocyte, in mice. Our half-cell proteomics data supports that this difference is possibly due to heterogeneities in ooplasm composition and how they are apportioned into the blastomeres depending on the orientation of the first zygotic division. Thanks to the co-Authors, to the Reviewers, to the Journal, and I hope you like the paper!

@HAIYANGWANG6 @NatureAging @MBIsg @ACRLE_NUS @NUSingapore Cool paper! And a cool method!

My paper on rejuvenating aged oocytes has been selected as the cover story for the latest issue of @NatureAging! 🌟 Grateful for this recognition! @MBIsg @ACRLE_NUS @NUSingapore #Oocyte #IVF #ReproductiveLongevity #AgingResearch x.com/NatureAging/st… nature.com/articles/s4358…

@ControllingLMNT @EwelinaBolcunF For sure. Successful maturation does not guarantee a "good" egg, and a euploid egg does not guarantee a viable embryo, right? Still don´t know where the reduced maturation rates in AMA oocytes I find in papers come from...

@FulkaLab @EwelinaBolcunF After oocytes reach a dev stage, it’s almost impossible for them not to undergo spontaneous maturation when granulosa cell cGMP transfer is lost. Gross maturation is essentially meaningless, no? Better to look at egg activation and cleavage IMHO.

How does one identify an oocyte with good developmental potential? Contrary to the often-reported decrease in maturation rate with advancing age, we never observed any difference. Has anyone truly witnessed this, or is it just the result of pressure to meet expectations/publish?

@zaffagg3 I have been working with AMA mice (>10months) for something like two years and don't see a difference. Sometimes, I would say they "mature" even slightly better than young ones. So I am wondering where the data comes from, I found it in so many papers.

@FulkaLab Idk if I have quantitative data but yes, in my hands aged oocytes (10-12mo) generally matured well (at least in terms of PB extrusion)

@zaffagg3 Yeah, in mice. It is in a lot of papers, but I just never saw it (not even in the same strain)... like, never.

@FulkaLab In mice you mean?