RutaGeras

STED microscopy enhances the spatial resolution by reducing the effective fluorescence emission volume, disrupting the linear relation between excitation and spontaneous emission probability. Find out how in this weeks Primer: go.nature.com/4dD0PYX

#TeamDonut🍩 entering Heidelberg Castle 🏰 for the Castle Dinner in the King‘s Hall 👑 #EESImaging

The @StefanHellLabs family at #SMLMS2023 — 🍩's past and present united! @AndImaging, R. Gerasimaite, @IlariaTesta4 @LOstersehlt @VicidominiLab @BalzarottiFran, I.Jansen, @RLincolnChemist @ElisaDEste, J.O.Wirth, S.J.Sahl, @wmarkbates @bewersdorflab Missing:@JGDanzl & @SSchnorren

Rhodamines exist in two forms: Zwitterion (fluorescent) and Spirolactone (non-fluorescent). Zwitterion is hydrophilic and likes water, but Spirolactone is hydrophobic and keeps its tail out of water. See our visualization of these two forms and enjoy #FluorescenceFriday !!!

Our group wish all of you Merry #Christmas and #HappyNewYear ! We wish all of you good health and peaceful cozy celebrations. Enjoy our postcard made of amazing structures which we observed in living cells. #AcademicTwitter #SciArt #FluorescenceFriday #Christmas2022

Best fireworks ever!

Please RT ! We are looking for a seriously motivated #PhD student to join in 2023. Join us, if you are passionate about chromatin labeling and imaging using #STED and #MINFLUX microscopy! Apply to @IMPRS_GS: uni-goettingen.de/de/application… #AcademicTwitter #PhDposition #Research

Back to #imaging and testing of lice-cell probes. #Centrosome and primary cilium in 3D #STED. Today is 11.11, and we wish you a happy #FluorescenceFriday ! #Science #SciArt #RealTimeChem #Chemtwitter

Nice and glowing. Coumarin 6 is a derivative of one of the coumarin. Coumarins' chemical synthesis was started in 1868 by W. H. Perkin. #FluorescenceFriday #fluorescentfriday #Science #SciArt #RealTimeChem #Chemtwitter

While we enjoys the western fascination with Halloween we have our own quirky commemoration. On November 1st Lithuanians go to cemeteries to light candles on the graves of their loved ones... Old cemeteries become a sea of lights. It's spooky yet mesmerizing experience.

Sometimes chemical reactions can be very bright and glowing! Happy #FluorescenceFriday or #FluorescentFriday! #SciArt #GLOW #RealTimeChem #Chemtwitter

Abstract writing template by nature @PhDVoice @PhD_Genie @yourPhDpal @phdfriend

"In order to demonstrate the repeatability/reproducibility of the method authors are asked to include relative standard deviations (RSD) or coefficient of variation (CV) in Tables." The Editor's comment to a review paper with no quantitative data. Publishing process is broken?

We have a new #chemistry lab at @mpi_nat which is nice to use for the synthesis of fluorescent probes. Let's start #FluorescenceFriday ! Enjoy ! #biology #photography #imaging #sciart

Excellent application of HMSiR-Hoechst probe: Biophysical ordering transitions underlie genome 3D re-organization during cricket spermiogenesis biorxiv.org/content/10.110…

New biocompatible probes for actin show more dynamics! We see not only F-actin, but also G-actin! Read more about old Jasplakinolide probes and their improved versions here: pubs.rsc.org/en/content/art… and biorxiv.org/content/10.110…

It was a great pleasure to work on this colorful project!

Inflation has also hit our group! By putting sanctions on the number of synthesis steps, we got the following image. Need more information, read here: biorxiv.org/content/10.110…

Moving towards Addprobe (@Addgene analog) = cheap and highly efficient synthesis of > 20 rhodamines' 4-isomers and >80 probes. Let's stain everything! Enjoy live cell movie Vimentin (cyan), DNA (green), tubulin (yellow) and mitochondria (red-hot)! Read: biorxiv.org/content/10.110…

Useful reading about MINFLUX: