Tim Grant

Making cryo-EM grids by flying particles through a native mass-spec is pretty cool, but can it be cooler? Yes - by adding a LASER! Particles damaged by the process can be fixed by adding a thin layer of ice then briefly hitting the grid with a laser! tinyurl.com/49aurm35

Learning how to process data using cisTEM and Relion at the cryoEM @cshlcourses ! @GrantCryoEMLab

Congrats to @tommy_anderson2 on his first author publication from our lab detailing the first structure of a polymerase complex from an alphacoronavirus, PEDV! Also a big shoutout to Josh Coon who helped with the native MS analysis! academic.oup.com/nar/advance-ar…

1/6: #CryoEM Exhausted with fighting the air-water interface (AWI) problem during sample grid plunge freezing? We may have an easy solution for you! Read our latest preprint on how we use LEA proteins as AWI sample protectants for challenging samples: biorxiv.org/content/10.110…

Great to have Pete in the lab!

nature.com/articles/d4158… I feel there can't be many people who have a quote containing colorful language in a journal?

@SjorsScheres @F4ustus You can get this on t-shirts - I really need to get one! 😀

Apparently people can get very emotional about #cryoem software. @GrantCryoEMLab

Congrats to those from our Morgridge community, @RomaBroadberry from the @GrantCryoEMLab and @kyle_flickinger from the @JasonRCantor Lab! 👏 #FearlessScience #RisingSparks

Congratulations @RomaBroadberry! Very proud

Congrats to the IPiB students who received poster awards at the IPiB Retreat! Roma Broadberry (Grant Lab) Jess Davidson (Simcox Lab) Kyle Flickinger (Cantor Lab) Evelyn Okal (Raman Lab) Phoenix Shepherd (Kirchdoerfer Lab) More photos from the day: flic.kr/s/aHBqjAVhZi

An Oct. 2-5 workshop @WIDiscovery will bring together #MassSpec and #CryoEM scientists to explore the possibilities and challenges of these novel techniques. Registration is open. For more info, visit coonlabs.com/ms_23/. @Coon_Labs @erwright73 @GrantCryoEMLab

We're #hiring! Join our department as a Professor of Biochemistry (open rank). Visit go.wisc.edu/biochem-facult… for more info and to apply. #FacultyJobs #PhDJobs #Biochemistry #TenureTrack

I love it when proteins move a lot! In our just published structure of DNA replication restart proteins PriA/B + replication fork (nature.com/articles/s4146…) we see one domain swing almost 90° upon DNA binding, turns out this is important. Awesome collaboration with @kecklabuw.

Our latest publication reports the cryo-EM structures of the human Polα-Primase DNA elongation complexes - rdcu.be/c96yY A Lim lab X Tahirov lab collaboration led by @QixiangHe and Andrey. Thank you, @sarakosman & @NatureSMB, for working with us! Please RT to share! 1/4

Kicking off the 2023 ⁦@CSHL⁩ #CryoEM course! Participants have braved flight delays, train fires, and long immigration lines to be here, looking forward to the next 2 weeks!

@UWMadisonRNA I'll put it on the requested features list! 😀

@KevinCFelt I'm not familiar with Phenix map sigma value comparison? Diffmap just scales two maps (both by resolution and in real space) and tells you the difference. It's very important to scale the amplitudes to get accurate comparison.

@GrantCryoEMLab for cryoEM map comparisons, would you say that Phenix map sigma value comparison and the Diffmap script from the Grigorieff Lab fulfil the same function?

@dmborek @SjorsScheres @DTegunov @DavidGilmore3 @biochem_fan @zo_lab @AnalyticsLigo Is this the software that uses some cisTEM code?

@SjorsScheres @DTegunov @DavidGilmore3 @biochem_fan @zo_lab @AnalyticsLigo It wasn’t patented.

"Ligo Analytics" ligoanalytics.com Apparently there is yet another startup company trying to make a software suite for CryoEM data collection, image processing and model building.

Attend next week's International Steenbock Lectures in Biochemistry to hear from Sjors Scheres @MRC_LMB on developments in #cryoEM and what cryo-EM can tell us about #amyloid filaments in the brain. Livestream: go.wisc.edu/bio_colloq @SjorsScheres @GrantCryoEMLab

From Alexis Rohou (@AlexisRohou) of @genentech and colleagues, a video article on how to configure gP2S, a web application for the tracking of cryoEM experiments. Watch the video on JoVE: hubs.ly/Q013j9xW0