Hui Wang

TomoNet is now published in Biological Imaging! It is a 100% GUI-based cryoET integration pipeline package. Specifically, we offer two major options for high-throughput particle picking to achieve high-resolution STA. Please check the website for details. #cryoET #teamtomo

@mimi1inh @NatureSMB @leonie_anton @mehsehret @Wenjing_Cheng_ @DziekanJerzy @CowmanAlan Looks very cool!

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @NatureSMB🥳rdcu.be/eBbrH Massive team effort led by @leonie_anton @mehsehret @Wenjing_Cheng_ in collaboration with @DziekanJerzy @CowmanAlan #teamtomo

Our study on the atomic structure of the T. brucei microtubule, has been published in Science and featured in UCLA News! This work was led by Dr. Xia @xianxia13 in collaboration with colleagues from Dr. Kent Hill's and Dr. James Wohlschlegel's labs. newsroom.ucla.edu/stories/atomic…

Excited to share our latest work: Using cryoET, we unraveled the in situ structure of a methanogen surface layer and its cell envelope. Now published in Science Advances! #teamtomo science.org/doi/10.1126/sc…

@RuiZhangWUSTL Thanks for sharing!

I want to take the opportunity to mention that I've written a slightly more user-friendly tutorial for the original microtubule seam-search protocol, together with other useful MT processing tricks. evernote.com/shard/s178/sh/…

@songli_UCLA @BioEngUCLA @dinodicarlo Thanks for the Excellent works!

It was a privilege and a rewarding experience serving as the department chair @BioEngUCLA in the past 8 years. I’m so proud of the department growth and achievements. Thanks to our faculty, staff and students! We will continue to thrive under the leadership @dinodicarlo

Our preprint of TomoNet is online now!

Here is the movie showing the hierarchical organization of the sheath layer, and how the giant sheath layer assembles from only one single SH protein.

Our paper is out! We resolved the sheath structure from M. Hungatei cells using cryoET. ⁦⁦Thanks to all the coauthors @zhou_lab⁩ and Gunsalus lab.⁦#TeamTomo nature.com/articles/s4146…

Check out the recent cryoET work with our beloved colleagues in Hill lab on the flagella structure of T. Brucei. nature.com/articles/s4146…

Check our preprint on the FAP106 study in T. brucei

@ChrtienDenis1 @EdoardoDImprima @NatureComms The current Isonet version only consider single axis tomograms.

@subletterdave @yuntaoinnit @zhou_lab Isonet doesn’t produce tilt series yet. One may could project tilt series based on the predicted volume to have corresponding TS, but still have missing wedge in pseudo subtomograms. So some extra work need to be done in the future

@yuntaoinnit @zhou_lab Does Isonet produce tilt series as well as tomograms? Relion uses tilt series to generate pseudo-subtomograms (in Relion 4 at least). May have to use a program other than Relion 4 to generate IsoNet subtomograms.

Isonet paper is published online now on nature communications! Congrats to Yuntao @yuntaoinnit and Heng!

Cryo electron tomography with Volta phase plate reveals novel structural foundations of the 96-nm axonemal repeat in the pathogen Trypanosoma brucei dx.doi.org/10.7554/eLife.…

We used #cryoEM to identify the proteins that form mammalian doublet microtubules in bovine respiratory cilia and contribute to the motility of cilia @miao_gui @jacobranderso @JosephineBotsch sciencedirect.com/science/articl…

The flagella of some single-celled algae generate a lasso-like beat pattern that propels the cell through water elifesciences.org/articles/67701…

OUT NOW: Bluetongue virus capsid protein VP5 perforates membranes at low endosomal pH during viral entry @zhou_lab @xianxia13 @UCLA @LSHTM Read it here: rdcu.be/cAiHD nature.com/articles/s4156…

The #DeepFinder paper is online @naturemethods! Paper📜 nature.com/articles/s4159… Code🧑‍💻 gitlab.inria.fr/serpico/deep-f… Press📰 pioneercampus.org/themenmenue-re… New #DeepLearning🤖🧠 software for identifying molecular complexes inside cellular tomograms. I hope it helps #TeamTomo. Try it out!!