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Inga Songailiene
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Inga Songailiene retweetledi
Genome delivery of a contractile tailed phage and its superinfection exclusion mechanism | bioRxiv biorxiv.org/content/10.648…
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@crypticprophage No, we cited your work in ApeA paper because we respect it.
Regarding the mechanism - we described tRNA 3'-end cleavage activity which your mentioned TA is not doing.
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@IngaTrails For Eco2: "The mechanism of Eco2 appears to be akin to that of the HEPN–MNT toxin–antitoxin system, ..." but you fail to cite the 1st ref for the mechanism for this TAs. You also fail to cite the 1stTAs that inhibited phage. For ApeA, you were forced to cite the prior lit.
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Toprim is different domain than HEPN. MNT can fit in here.
tRNA cleavage expriments from our MNT-HEPN study really helped for Eco2. The study:
pubmed.ncbi.nlm.nih.gov/33290744/
Cryptic Prophage (phage 🧬🪤 in 🦠)@crypticprophage
nature.com/articles/s4159… Retron Eco2, another derivative of TA system HEPN-MntA (doi: 10.1093/nar/gkaa855), works as a trimeric complex that is activated by T2 phage endonuclease DenB, which cleaves msDNA & leads to RNA degradation.
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@crypticprophage what do you mean? Eco2 has nothing to do on AMPylation. We cited your paper on ApeA HEPN study, our study complements your findings in NAR and adds extra info about tRNA cleavage that was relevant to Eco2 paper.
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@IngaTrails Point remains, which you concur, Eco2 may be a derivative of the HEPN-MntA TA system, and yours was the 2nd report of this MntA AMPylation type VII and ours was the first and was not cited.
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@crypticprophage Toprim is different domain than HEPN. MNT can fit in here.
tRNA cleavage expriments from our MNT-HEPN study really helped for Eco2. The study: pubmed.ncbi.nlm.nih.gov/33290744/
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nature.com/articles/s4159…
Retron Eco2, another derivative of TA system HEPN-MntA (doi: 10.1093/nar/gkaa855), works as a trimeric complex that is activated by T2 phage endonuclease DenB, which cleaves msDNA & leads to RNA degradation.
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Deoxydinucleotides can activate doughnut-shaped mutimeric HEPN ApeA nucleases for tRNA cleavage in the anticodon loop ✂️🛡🧬
Great work lead by Jonas, Giedrius and great team 💪🙌
Jonas Juozapaitis@JJuozapaitis
🧬 We discovered how bacteria detect phage infections by sensing fragments of their own degraded DNA. Meet #ApeA – a #BacterialImmunity system activated by a novel signal - #Deoxydinucleotides. Read our preprint: biorxiv.org/content/10.648… A thread 🧵 (1/11)
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Inga Songailiene retweetledi
Happy to share that our paper on the PlPVC1 eCIS is out in @NatureComms 🥳 Congratulations to first author Leyre Marín-Arraiza and all co-authors! #cryoEM @cryoEM_Papers @UCPH_CPR nature.com/articles/s4146…
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Impressive spacer selection mechanism by Cas9-adaptation supercomplexes - all pathway captured by #cryoEM #Cas9 🧬🧩🧵
Giedrius Sasnauskas@GiedriusSasn
'Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation' Check out our preprint on #bioRxiv on the #Cas9-Cas1-Cas2-Csn2 supercomplex involved in #CRISPR spacer acquisition: biorxiv.org/cgi/content/sh… Thread below.
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Inga Songailiene retweetledi
Excited to see our work published in @ScienceAdvances!
We present RBPseg, a method for structural prediction of #phage receptor-binding proteins.
We applied it to diverse tail fibers and validated the predicted structures using #cryoEM.
Read here: science.org/doi/10.1126/sc…
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Inga Songailiene retweetledi
I highly recommend attending this amazing microbiology conference!
🦠🔬👩🏼🔬🧬🧪
Only a few more days to submit your abstracts! (April 1st ).
Looking forwards! 🍻
EMBL Events@EMBLEvents
⏳ The #EESMicrobiology abstract submission deadline is fast approaching ⏳ 🦠🔬 Don't miss the chance to present your research to top scientists from around the world working with bacteria and new technologies! Submit your abstract by 1 April 👉 s.embl.org/ees25-06-x This EMBO | EMBL Symposium will focus on new approaches and technologies that have the potential to revolutionise future microbiology research, from systems-based to single-cell 🧬 📅 24 – 26 June 📍 EMBL Heidelberg and Virtual #EMBLEvents #Microbiology
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Inga Songailiene retweetledi
RNA-guided systems span kingdoms, from bacterial immunity to eukaryotic gene regulation. How did they evolve? We traced the origins of CRISPR-Cas13 and identified its likely ancestor: an RNA-based toxin-antitoxin (TA) system. Here’s what we uncovered:
cell.com/cell/fulltext/…
1/8
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Inga Songailiene retweetledi
I can add "Life's Engines: How Microbes Made Earth Habitable" by Paul G. Falkowski.
What more can you recommend for next year?
Eric Topol@EricTopol
My favorite books, 2024 by @emollick @firstadopter @anilananth @harari_yuval @carlzimmer and other great authors not on X
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@TaraBartolec @KMitosch @savitski_lab @TypasLab Thank you for testing MNT-HEPN, ApeA and including them into the study! The mechanisms of activation for these systems still remain mysterious.
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1/11 - With @KMitosch and Clément Potel + colleagues in @savitski_lab @TypasLab @embl, I’m very happy to share our discovery of a hyper-promiscuous phage protein kinase (T7K) which broadly deactivates bacterial defenses! biorxiv.org/content/10.110…
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Inga Songailiene retweetledi
Happy to share our spotlight (with @g1_guillermo at @UCPH_CPR) in @CellChemBiol, highlighting the groundbreaking work by @IlyaOsterman & @SorekLab on how #phages reconstitute NAD to bypass bacterial immunity🦠🛡️. Check it out here! rb.gy/n8w9na 🤓
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Inga Songailiene retweetledi
Excited to share our latest collab with Wenyuan Han's Lab! 🚨 Bacteria use dITP as an immune signal to activate NAD+-depleting effectors. Phages counter immunity by destroying dAMP (dITP precursor)! 💥🧬
Preprint 🔗: biorxiv.org/content/10.110…
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Inga Songailiene retweetledi
Thrilled to share that our Zorya paper is now published online in Nature! It was an incredible team effort @__jphilipp @Salmo_Lab @SimonJacksonNZ —thank you to everyone who contributed to this work.@IngaTrails @VKleinSousa @AritzRoa @freddie__martin @yongwangKU @AHarms485 @others
Nicholas M. I. Taylor Lab@NMITaylorLab
Very happy our paper on Zorya anti-phage defense system is now published in @Nature! 🥳 Big congrats to first authors @HaidaiHu and @__jphilipp and all other authors! Wonderful collab with @Salmo_Lab and @SimonJacksonNZ #cryoEM @UCPH_CPR @koebenhavns_uni nature.com/articles/s4158…
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