Michael Totty

Very excited to share my second postdoc paper with you all, now out in @ScienceAdvances! We used targeted snRNA-seq to profile four major subnuclei of the primate across three species (including humans). 1/14 science.org/doi/10.1126/sc…

New leaked Epstein documents show the FBI removed the hard drive from the prison's camera system and wiped all footage from the night Jeffrey Epstein was murdered..

Stress undermine behavioral therapies for PTSD; beta-blockers may alleviate this problem. We hope that this work in animals will inform clinical interventions in humans. Thanks to @NIH for supporting the work and my lab group @BeckmanIllinois for this outstanding effort!

Please stop insulting the centuries of effort put in by biologists & computational biologists who built those prior knowledge networks & literature that u piggy back on by calling ur nearest neighbor look up engine a virtual cell. 26/

By tracking nearly every movement of a tiny fish’s life from adolescence to death, a new Science study reveals a hidden behavioral blueprint of aging—one that can predict a fish’s age or how long an individual will live. Learn more: scim.ag/4dm1GA9

Vanderbilt vs. the actual 4 seeds—hard to figure

Vanderbilt & Michigan State: both No. 4s in bracket preview on Feb. 21 MSU: one Q1A win since then | 2 wins over tourney teams. Rose to a 3 Vandy: three Q1A wins | 4 wins over tourney teams. Fell to a 5 Brand bias at its worst. cbssports.com/college-basket…

This is wild. theaustralian.com.au/business/techn…

Spatial perturb-seq: single-cell functional genomics within intact tissue architecture Scale up your in situ CRISPR screens Out in @NatureComms

@EphysTanner Cool paper! But shifts in neural dynamics due to new learning (extinction) is not representational drift.

New Kaye Lab preprint is out now! We used longitudinal dendritic spine imaging, synaptic proteomics, mini scope calcium imaging, and electrophysiology to study MDMA-induced plasticity and representational drift during fear extinction! 🔗⬇️

The empty drops you threw out in your single-cell RNA sequencing analysis might be hiding mysterious things 👀 check out our bioRxiv preprint! The empty drops do not contain cells. Yet we can still use them to learn interesting things about biology and technology. 1/

How many samples should you sequence? Collect too few, and the experiment is inconclusive. Collect too many, and the costs add up very quickly. Check out our bioRxiv preprint and calculator at poweranalysis-fb.streamlit.app! 1/

Finally, it allows us to put a number on the intuition that getting a p-value of 1e-100 at a l2fc of 0.5 requires on the order of hundreds of samples, and if this test is reported in a sample with n=5, something terribly wrong may have happened. 6/ x.com/GorinGennady/s…

Powerful: Texas A&M wide receiver KC Concepcion on why he decided to talk to the media at the NFL combine despite having a stutter. “I want to be a role model for those who are not able to speak in front of large crowds.” KC is a true role model ❤️👏

🚨 Today in @Nature, we report GEMINI—a genetically encoded intracellular memory device that writes cellular dynamics into tree-ring-like fluorescent patterns within cytoplasmic protein assemblies.[1/n] nature.com/articles/s4158…

After years of work, the centerpiece of my PhD is published in @NatureMethods! Read it to learn about the biophysical insights we can get from single-cell data! But first, I would like to talk a bit about RNA velocity and normalization. 1/

Inspirational: Texas A&M wide receiver KC Concepcion speaks out about the negativity he's received for stuttering at the NFL combine. “I wanna be a role model for those who may be scared to speak up, who may be afraid & not confident in yourself… Don’t let an outside person’s thoughts, opinions get in the way of you being great, of you achieving something in life.” A role model on and off the field ❤️🙏

Terrible: People have been making fun of Texas A&M wide receiver KC Concepcion for the way he spoke while answering questions at the NFL combine. KC has a speech impediment and has been stuttering his whole life. Give him praise, not negativity ❤️👏

1/8 We learned what kinds of neurons are in the brain with Drop-seq, and where they are located with Slide-seq/tags. But what about their synaptic anatomy?

New research shows how the #brain links environments with appetite & responses to food.This contextual linkage helps explain how healthy individuals control appetite, but in an unhealthy state, can lose control over eating—as is seen in #eatingdisorders. ctt.ec/ea701+

In 1999, Tom Maniatis discovered something remarkable: neurons achieve self-avoidance via stochastic methylation of the protocadherin gene cluster. We've just discovered this locus is an evolvable in-vivo barcode across the human tissues: biorxiv.org/cgi/content/sh… 🧵