Osvaldo Burastero

Scientists from EMBL Hamburg and CSSB have provided key insights into the cellular process of clathrin-mediated endocytosis. They revealed details on specialised functions of two adaptor proteins involved in the process. embl.org/news/science-t…

@Osvalburastero providing a tutorial on eSPC, our online data analysis platform for molecular biophysics in the frame of “ Towards data FAIRness in molecular biophysics” conference in Prague, CZ. Data access and storage as a relevant topic to address in the biophysics field.

🔊 I am delighted to share our latest paper “Subtleties in Clathrin heavy chain binding boxes provide selectivity among adaptor proteins of budding yeast” Great collaboration with our colleagues from the @CssbHamburg @SkruznyMichal Our publication ⬇️ rdcu.be/dZujI

Big thanks to the original developers for creating code that's both reusable and easy to understand!🙌(github.com/rubiscoo/Dichr… and mpinat.mpg.de/sesca).

More updates to our CD tool (spc.embl-hamburg.de/app/chirakit) 🥳 - CD spectra prediction from protein structures (Nagy, et al. 2019) - Bayesian secondary structure estimation (Nagy, et al. 2021) - Thermal unfolding for helical peptides (Zavrtanik, et al., 2024)

Working with dimers and Circular Dichroism? We updated our Chirakit tool with more unfolding models (spc.embl-hamburg.de/app/chirakit). All feedback is welcome! :) The image below shows a global fitting (N2 <-> I2 <-> 2U) of data from Figure 3A, Felipe Montecinos-Franjola et al., 2012.

@claudialenamaro @instructhub @ERIC_forum Thank you Claudia!!

Congratulations Osvaldo! Keep us informed of developments and well done! @instructhub @ERIC_forum

Thanks a lot @MOSBRIeu and @kemijski for the fantastic conference!

Our latest submission to @biorxivpreprint is now online. biorxiv.org/content/10.110… Huge thanks to all authors @LucasADefelipe @SkruznyMichal @uetrechtlab @RolandTh1 @katharinchen5 @Osvalburastero @IsabelTBento and contributors involved in this collaborative work.

Join #EMBOPEPC to learn how to troubleshoot in the wet lab during solubilisation and reconstitution, and how to conduct experiments at the beamlines and during early stages of cryoEM screening. 🔎⚡️ ✍️ Apply by 17 June 📍 EMBL Hamburg ➡️ s.embl.org/pep24-01 🔸 Speakers and Trainers Lucia Banci, University of Florence Clement Blanchet, @EMBLHamburg @Osvalburastero, EMBL Hamburg @LucasADefelipe, EMBL Hamburg Juan Du, @VAInstitute Jens Frauenfeld, @SaliproBiotech @MelissaPoynor, EMBL Hamburg @Steve_Harborne, @PeakProteins @ProfSyK, @UniofOxford @jankosinski, EMBL Hamburg @kirkovalev94, EMBL Hamburg @linamalinama1, @VU_LT @MarlovitsLab, @UKEHamburg and @CssbHamburg @Mel_McDowell22, @MPIbp Pedram Mehrabi,@MPSDHamburg @stemuench, @UniversityLeeds Stephan Niebling, EMBL Hamburg Arwen Pearson, @unihh and @MPSDHamburg Martin Pelosse, @EMBLGrenoble @KimRemans, EMBL Heidelberg Mikhail Savitski, EMBL Heidelberg Eike Schulz, @UKEHamburg Markus Seeger, @seegerlab, @UZH_en @BettinaSiebers, University of Duisburg-Essen Henning Tidow, @unihh Maya Topf, Centre for @CssbHamburg and @LeibnizLIV Charlotte Uetrecht, @uetrechtlab, @CssbHamburgy and @LeibnizLIV Katharina Veith, EMBL Hamburg David von Stetten, EMBL Hamburg Yexin Xie, @EMBLHamburg 🔸 Scientific Organisers Maria Garcia, EMBL Hamburg Simon Mortensen, EMBL Hamburg Kim Remans, EMBL Heidelberg Angelica Struve, EMBL Hamburg Henning Tidow, @unihh #membraneproteins #structuralbiology #cryoem #saxs #macromolecularcrystallography #crystallography #biophysicalcharacterisation #cellbiology #spectrometry #xraydiffraction #enzymaticreactions #proteinmodelling

It has features for basic spectral processing, thermal/chemical unfolding, secondary structure calculation, spectra decomposition (PCA/SVD), and more...

Working on circular dichroism? Try ChiraKit, the latest addition to our eSPC platform. Available at spc.embl-hamburg.de A great collaboration between the SPC facility, EMBL Hamburg & the ISA, Department of Physics and Astronomy, Aarhus University, Denmark.

Do you know our next PhD students? We have two openings starting this summer, one for engineering of biomolecular condensates, and one for de novo design of protein binders (and their applications). Link and details ⬇️

The call for applications to the ARISE Fellowship Programme closes on 30 September! Do you love working on technology development? ARISE trains scientists & engineers for careers in research infrastructures. Learn more & apply now: embl.org/about/info/ari…

Raynals is user-friendly software for the analysis of single-angle dynamic light-scattering data that uses the Tikhonov–Phillips regularization and is free for academic use @IUCr #DynamicLightScattering #MolecularBiophysics #OnlineDataAnalysis doi.org/10.1107/S20597…

@SPC_EMBL_HH @EMBLHamburg @institutpasteur @embl @MOSBRIeu Try the tool at our online platform spc.embl-hamburg.de and don't doubt to send us feedback so we can improve it!

Our new tool for the analysis of Dynamic Light Scattering (DLS) has been published!🥳🥳 journals.iucr.org/d/issues/2023/… Kudos to the @SPC_EMBL_HH, @EMBLHamburg and the Plate Forme de Biophysique Moleculaire, PFBMI, @institutpasteur, and many thanks to the founders (@embl & @MOSBRIeu)

Join me and my team developing X-ray imaging methods and have some fun in Hamburg at the same time!

Excellent training and great opportunity to meet the fellows from the other sites. Thank you @embl!

Latest publication of our Fraunhofer user, Tania Szal et. al, showing the aggregate effect of efflux pump inhibitors on ArcAB-TolC complex in E.coli! @embl @EMBLHamburg @FraunhoferITMP @CssbHamburg @UKEHamburg doi.org/10.3390/biom13…