Ting-Feng Lin

Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.110…. We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.

Hi #neuroscience! As I transition into my new role as an Assistant Professor at Utrecht University in the beautiful Netherlands, I’m excited to share that I’ll soon be recruiting a PhD student to study cerebellar neural plasticity and development using larval zebrafish.

Our paper "Optimizing gRNA selection for high-penetrance F0 CRISPR screening for interrogating disease gene function" is now published in @NAR_Open doi.org/10.1093/nar/gk…. This work was led by our super postdoc @ShengJiaLin9413 * Several papers on F0 mutagenesis have been published, including those by Jao et al., Burger et al., Wu et al., Kroll et al., and others—all methods work well. We set out to test and compare strategies to achieve the highest phenotype penetrance using minimal resources. * We utilized indel prediction models such as Lindel (or inDelphi/ForeCast and others) to design gRNAs with high predicted success. * Targeting the N-terminal half of functional domains worked best. We tested 324 gRNAs across 125 genes, targeting key protein domains with just 1-2 gRNAs. * Our data shows that targeting the N-terminal half of a functional protein domain significantly increases phenotypic penetrance compared to targeting other regions of the gene. Selecting gRNAs with high Lindel scores (>75) substantially improved knockout efficiency. * Transcriptomic analysis revealed that gene expression patterns in F0 knockouts closely mimic those in stable knockout lines. * We validated 37 candidate disease genes across 8 tissues and screened 63 hearing-related genes, identifying 52 with hearing defects. Also, we identified 10 new neurodevelopmental disorder (NDD) genes in zebrafish.

Developmental synaptic pruning in the olivo-cerebellar circuit sculpts predictive processing biorxiv.org/cgi/content/sh… #biorxiv_neursci

How do zebrafish map their environment? A new Nature paper by @rolilab found first evidence for place cells in fish. These specialized neurons play a crucial role in forming mental maps of space, social networks, and abstract relationships. 👉kyb.tuebingen.mpg.de/place-cells