Guo-Liang "Chewie" Chew

@ribonucleicacid The paucity of As and Ts in the first exon is likely due to the depletion of start (ATG) and stop (TAA, TAG, TGA), all of which are AT rich. This general depletion of uORFs is observed quite generally.

This plot of nucleotide content along human protein-coding genes (from 5' to 3' ends), with exons in yellow and introns in white, has many secrets hidden inside. I'm building a model of gene evolution. It explains many of these trends, but other patterns are still mysterious.

@eugenevalkov Isn't this similar to the (formerly Ambion) Bright star BioDetect system? I've previously used this to detect 30nt RNA footprints

@johnprensner @StevenSalzberg1 @carninci I discussed this in the last chapter of my thesis some time ago: dash.harvard.edu/handle/1/17467…

LLMs are ideal models for molecular biology. Here is my new essay reviewing some recent advances and looking to the future. @manoliskellis @lindaavey @anshulkundaje @BoWang87 @StevenSalzberg1 @illumina @chrmanning @slavov_n towardsdatascience.com/large-language…

Introducing PrimateAI-3D, a significant step forward in our use of deep learning to identify genetic mutations most likely to cause disease. Learn more about the groundbreaking research behind the updated algorithm, published today in @ScienceMagazine. illumina.com/company/news-c…

Interesting study of UK Biobank exomes for common traits. In aggregate, rare variants still explain much less variance than common PRS (note scale is corr), but can already better identify phenotypic extremes (top 0.1%). medrxiv.org/content/10.110…

@ShechnerLab I got Psoralen-biotin (Ambion BrightStar) to work during my grad school days, both for small (~30nt) and large mRNAs. Caveat: probe was to EF1A, so a very abundant mRNA. Could work for your case though...

Hallo fellow RNA Nerds! We’re in need of a working protocol for non-radioactive northern blots. Those probing ribosomal precursors are *especially* welcome. Anybody got something working?

@Ruchi_P_Jain @shifengxue @ZebrafishRock I agree, I believe the Sive lab had a technique for brain ventricle injections (PMC2791086)

@shifengxue @ZebrafishRock Could be Peptidylglycine α-amidating monooxygenase (PAM), similar staining seen in an article from 2018.

#zebrafish experts, we mixed up a probe recently and got this pattern at 24hpf. What is this diamond-shaped structure in the head and what could this probe be (might be 2 probes)? Or is it just super intense probe trapping? #GuessThatISH @ZebrafishRock

LEUKOCYTE: a complete musical overview of the human immune system hope @bts_bighit don't mind :)

Groundbreaking research by Mo Ameen and Laksshman Sundaram in @Cell highlighting AI to diagnose non-coding de novo mutations in congenital heart disorders, with Anshul Kundaje, Will Greenleaf, Thomas Quartermous, Kevin Wang, Ioannis Karakikes at @Stanford: cell.com/cell/fulltext/…

@DrimisWinteri @Ohnolog @LaboJeanPerrin @rolilab

We made this giant #zebrafish larva model at @LaboJeanPerrin to show kids how a zebrafish larva swim. Green leds are supposed to be neurons. Isn’t it amazing? 🤩

@mbeisen @MarshallJulius *Obi-Two Cannoli

@MarshallJulius No. That's Obi-Wan Cannolo. This is Obi-Wan Cannoli.

Obi-Wan Cannoli

@aspens_grove Also depends on where the conference is, and the field in general. CSHL / Gordon conferences in the basic sciences are chill (I've attended them in shorts, even as a postdoc), but the more "medical" and "European" a conference is, the better people tend to be dressed.

@aspens_grove It doesn't get easier. You just get better.

@PauliGroup 🤯

@joel_nitta purrr.tidyverse.org/reference/map2…

@joel_nitta use pmap!

Would like to have two (or more) sets of dots (...) to pass in functions #RStats #my_druthers

@HsiehLab @illumina Thanks Andrew!

@chewomics @illumina Wow, congrats!

A big-ish announcement: I've just started a new position @Illumina, and I'm currently spinning up the Singapore branch of the Illumina AI lab.

Our's and other bioinformatics data science groups at Illumina Singapore are rapidly expanding in the coming months. If the prospect of using *big* data to make fundamental and impactful discoveries in genome biology excites you, please reach out to me.

We will develop and use best-in-class AI methods (including deep learning) to better understand the biological and clinical impact of human genetic variation, with a particular focus on Asian genomes in Singapore.