Nishit Goradia

Updated version Pathbreaking work! It has been quite a journey! biorxiv.org/content/10.110…

Our latest study of the scavenger receptor CD163, led by @RichardZhou_. This follows our previous study of how CD163 uses its remarkable molecular architecture to bind haptoglobin-haemoglobin. We now show how its flexible arms allow haemoglobin binding and ligand promiscuity.

In the latest issue! Thermodynamic prediction of RNA cellular activity from sequence via conformational ensembles dlvr.it/TSZGwz

Algorithm-driven, phenotype-directed bioactive molecular discovery dlvr.it/TSZFXW

Cancer-linked aggregation of p53 is driven by sequence-encoded frustration, solvation, and hydrophobic gating absent in its paralogs dlvr.it/TSZBHk

Mitochondrial components secretion in extracellular vesicles promotes alveolar epithelial mitochondrial quality control | Nature Communications nature.com/articles/s4146…

Mitochondria defend the nuclear envelope | Nature Chemical Biology nature.com/articles/s4158…

One of the most-viewed PNAS articles in the last week is “3D epithelial cell topology tunes signaling range to promote precise patterning.” Explore the article here: ow.ly/inf250YZGTe For more trending articles, visit ow.ly/F5iW50YZGTg.

FILM: a mid-IR photothermal microscopy method for gentle imaging of metabolic processes in cells and worms. @HHMIJanelia @HHMI_science nature.com/articles/s4159…

α-Synuclein aggregates induce mitochondrial damage and trigger innate immunity to drive neuron–microglia communication: nature.com/articles/s4146…

Guide DNA — not RNA — expands the CRISPR toolkit: nature.com/articles/s4158…

A genetically encoded peptide inhibitor of mTORC1 is developed and reveals that nuclear mTORC1 regulates a specific set of genes and promotes cancer cell proliferation nature.com/articles/s4158…

Ki-67 promotes circulating tumor cell intravasation and metastasis in breast cancer dlvr.it/TSYb73

休眠リボソームからの翻訳再開メカニズム 飢餓状態の細胞をCryo-ETで観察し、未知の電子密度から新因子のSNORを同定。SNORはリボソームに結合し、飢餓からの回復時にタンパク質合成を再開するライセンス因子として機能。欠損で休眠からの回復に異常 手法も因子も面白いです nature.com/articles/s4158…

DNA-guided CRISPR–Cas12 for cellular RNA targeting go.nature.com/49SP29d

🧬 One CRISPR editor. One step. Gene insertion + multi-site epigenetic silencing — with 1,000× fewer chromosomal translocations. The trick: gRNA length controls the mode. → 20nt gRNA = Cas9 cuts → HDR transgene insertion → ≤16nt gRNA = Cas9 binds but doesn't cut → KRAB+DNMT3A/3L epigenetic silencing In primary T cells: ✅ ~30% dual-edit efficiency (eGFP insertion + B2M silencing), stable >31 days ✅ NY-ESO-1 TCR knock-in + B2M silencing (82%) + TET2 silencing ✅ Translocation risk: 1,000× lower than triple-KO Nature Communications doi.org/10.1038/s41467… #CRISPR #CARTcell #CellTherapy #EpigeneticEditing #GeneEditing

I am very happy to share that our paper is now published in @Nature 😊 Cell-type-targeted mitochondrial transplantation rescues cell degeneration. nature.com/articles/s4158… @IOB_ch (1/6)

Genetically targeted mTORC1 inhibitor reveals transcriptional control by nuclear mTORC1: nature.com/articles/s4158…

Online now! Structural basis for the subtype-selective activation of KCa3.1 channels dlvr.it/TSXcB5

In situ structure of the human gap junction | Science Advances science.org/doi/10.1126/sc…

proteins aren’t static structures. they constantly sample rare higher-energy states that shape function, aggregation, and disease. this paper maps those hidden conformational fluctuations across thousands of protein domains at scale, opening the door to predicting protein energy landscapes instead of just native structures. nature.com/articles/s4158…