Lei Jin

A small step from 2025: “Comparative Study of BBB-Targeting AAV Capsids on Central Nervous System Delivery Efficiency | bioRxiv” biorxiv.org/content/10.648…

Online now: Protocol for recombinant rabies virus titration by quantitative PCR dlvr.it/TJP2fL #protocol #starprotocols #cellpress

Moved to a new space today

Neuroscientists @ecallaway @salkinstitute and @ian_wickersham @MIT wanted to develop a better way to map individual neuronal connections. They found an unlikely ally in a deadly neurotropic virus. Learn more: bit.ly/3RtVomr

Great article about our and others' rabies virus work in @TheScientistLLC, by @HannahThomasy: the-scientist.com/engineered-rab…

Research Highlight by Nina Vogt: @ian_wickersham and team developed a rabies virus-based tool for monosynaptic retrograde tracing of neurons in the rodent brain. This version is less cytotoxic than its predecessor and allows long-term experiments. nature.com/articles/s4159…

Neurons interact with others at synapses, but mapping these connections is a challenge. Aixin Zhang, @leijinmit, @XiaoyinChenLab, @ian_wickersham and others explore ways to “sequence” these connections using RNA barcoded rabies viruses. Read the study: elifesciences.org/articles/87866

A lighter read from eLife Digest introducing the paper. elifesciences.org/digests/87866/…

Our first paper on synaptic connectivity mapping using barcoded rabies, led by Aixin Zhang in my lab and @leijinmit, is now VOR on eLife. An absolute pleasure working with @ian_wickersham Shenqin Yao, Bosiljka Tasic on this. Looking forward to pushing this further!

Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing | eLife elifesciences.org/articles/87866

A second-generation system for labeling direct inputs to targeted neuronal populations in vivo with minimal toxicity, using double-deletion-mutant rabies viruses @leijinmit @ian_wickersham @mcgovernmit @MIT nature.com/articles/s4159…

A new tool developed by @ian_wickersham + @leijinmit gives neuroscientists the power to find connected neurons within the brain’s tangled network of cells, and then follow or manipulate those neurons over a prolonged period. @ScienceMIT @mitbrainandcog mcgovern.mit.edu/2024/01/11/map…

Our second-generation monosynaptic tracing paper is now online at Nature Neuroscience (open access): "Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses" nature.com/articles/s4159… 1/12

An @MIT team including Dr. @ian_wickersham introduce a 3rd gen of #rabies viral vectors which appear to be as nontoxic as 2nd gen ones but can be grown at much higher titers, allowing a significant increase in the number of retrogradely labeled neurons. bit.ly/3GqvutR

New paper from our lab finally officially published (with open access): "Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency" cell.com/cell-reports-m… 1/11

@winnieyangwn thanks

@leijinmit Congrats!🥳🥳

Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency: Cell Reports Methods cell.com/cell-reports-m…

BREAKING NEWS The 2023 #NobelPrize in Physiology or Medicine has been awarded to Katalin Karikó and Drew Weissman for their discoveries concerning nucleoside base modifications that enabled the development of effective mRNA vaccines against COVID-19.

Another updated preprint alert! This update is to our paper introducing "∆L" (third-generation, deletion of only the polymerase gene L) rabies viral vectors: biorxiv.org/content/10.110… 1/

#PeerReview from @eLife of 👉🏿 Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing #review" target="_blank" rel="nofollow noopener">biorxiv.org/content/10.110… #bioRxiv #reviewedpreprint