Simon Hardwick

@naturemethods has written a nice little synopsis of our recent SnISOr-Seq paper, including a chat with @hagentilgner: nature.com/articles/s4159…

@noush_joglekar @hagentilgner @NatureNeuro Congrats @noush_joglekar, @hagentilgner and all involved!

If you were looking for evidence that isoforms are cool, all-pervasive in the 🧠, and everybody should study them at the single cell level, our work on mouse development and brain-region variability from the @hagentilgner lab is now live @NatureNeuro! 1/ nature.com/articles/s4159…

@DJGould94 @noush_joglekar best umap plot i've seen in a while tbh

PhD students presenting their findings to their supervisor:

Only 2 days to go until the inaugural @ARNAconference kicks off in Thredbo! I'll be speaking in Session 2 on Monday morning 🧬 #arna #mrna

Research Highlight by @DrLeiTang: SnISOr-Seq enables cell-type specific isoform analysis in frozen samples. Original paper: nature.com/articles/s4158… Highlight: nature.com/articles/s4159…

Hey there #Bioinformatics folks looking for a job, I have an academic PI friend looking to hire bioinfx talent to work on long read (@PacBio Iso-Seq) + other omics. International candidates welcome! no job req yet so for now DM me to be connected. RT appreciated ❤️

.@si_hardwick & team present single-nuclei isoform RNA sequencing (SnISOr-Seq). Their method enables investigation of cell-type-specific splicing patterns & improves understanding of isoform combinations in healthy tissue & in disease: bit.ly/3wNrFvi

Imagine you could sequence one molecule on @PacBio and @nanopore ... you could really tell all the differences on a single-molecule basis, where any discrepancy must be a mistake in one approach: genome.cshlp.org/content/early/… Led by the incredible Alla M. & Andrey P. @genomeresearch

For the first time in the #covid pandemic, Australia now has more average daily cases than the US. Yet our average daily deaths is only 16, compared to 1,224 for the US 🤔

@RNASeqBlog has written a nice little blog post on our recent SnISOr-Seq paper :)

We used ReadUntil targeted @nanopore sequencing to solve tricky tandem repeat disorders. Read all about it: science.org/doi/10.1126/sc…

Amazing work led by postdocs Simon H, Wen H and grad student Anoushka J, with contributions by so many : nature.com/articles/s4158… Get splicing info out of human brain. TSS-exon, exon-polyA and distant exon pairing rules are more cell-type specific. Adjacent exon pairs less so.

Hot off the press! Latest paper from our lab is out now in Nature Biotech. Thanks to @noush_joglekar, @rainryuhei_wen, @hagentilgner and everyone else involved... nature.com/articles/s4158…

Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue go.nature.com/3sNrrlc

I am beyond thrilled to present our work on single-nuclei isoform sequencing (SnISOr-Seq), now out in Nature Biotech nature.com/articles/s4158…. Thread below ⬇️

Had a blast leading this project with @noush_joglekar, @hagentilgner and many others at Cornell and elsewhere...

Single-nuclei isoform RNA sequencing reveals combination patterns of transcript elements across human brain cell types biorxiv.org/cgi/content/sh… #bioRxiv

Our new work on single-nuclei isoform sequencing is available on biorxiv: biorxiv.org/content/10.110… Led by postdocs @si_hardwick & Wen Hu and PhDstudent @noush_joglekar but with contributions by many. Applied to human PFC and sc splicing, TSS, polyA sites and combination patterns.

Our new preprint is up! We develop and apply a new technique which enables single-nuclei, long-read RNA sequencing in human brain samples (or any frozen, archived, or hard-to-dissociate sample) @hagentilgner biorxiv.org/content/10.110…

We are really excited to share our work on a new modular RNA delivery technology called SEND, built from endogenous components found in the mammalian genome. @ScienceMagazine science.sciencemag.org/content/373/65…