Sofi Quinodoz

So incredibly honored and thrilled to have been selected as a @HHMINews Hanna Gray Fellow! Huge thank you to so many – including my incredible mentors @brangwynnelab @mitchguttman and colleagues @Princeton @Caltech who I have been so lucky to work with! 🧬👩‍🔬 #HannaGrayFellows

Combining sequencing and imaging, @sofiquinodoz maps the spatiotemporal dynamics of rRNA processing, demonstrating how rRNA serves as both a scaffold and a substrate for the nucleolus—a multiphase, liquid-like structure. nature.com/articles/s4158… @brangwynnelab @omenndarlingbio

Now on the Princeton Engineering and ODBI sites, a new mapping tool that allows researchers to peer deep into cells' inner compartments to watch and control ribosome assembly, shedding new light on the machinery responsible for making proteins.

Excited that our nucleolus mapping paper just came out today in Nature! Truly an amazing study from even more amazing due of @sofiquinodoz & @jiang_lifei w/ @LafontaineLab & Sebastian Klinge and other fantastic co-authors nature.com/articles/s4158…

@ShechnerLab @dschweppe1 @oligopain @shaoenong @berteroale Congrats Dave!! Super cool

@dschweppe1 @oligopain @shaoenong @berteroale And that's a wrap (for now)! I hope you've enjoyed this thread and give our preprint a read. Let us know what you think! (30/30)

It's my pleasure to present the next big preprint from SheqLab! An exciting application of our O-MAP platform that I hope will transform the study of nuclear architecture. If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)

@rawatravans Thanks so much!

@sofiquinodoz Hi Sofi, This is fantastic publication. Congratulations 🎊 Great job done and congrats to all authors.

Excited to share a new preprint! (1)🔬The nucleolus is the most prominent nuclear condensate, with a fascinating multilayered liquid-like structure, and is the site of ribosome biogenesis. But how does this multiphase architecture form and function? biorxiv.org/content/10.110…

@DavidWSanders2 @SuperSciJew @DrJorine @danielswlee @mitchguttman @_prashantbhat Thanks David 😊

@sofiquinodoz @SuperSciJew @DrJorine @danielswlee @mitchguttman @_prashantbhat Epic.

@CreminsLab Thanks so much Jenn! Definitely a big team effort 💪😊

It has been a wonderful journey uncovering the mysteries of the multiphase nucleolus🤯🤩! So lucky to drive this project together with amazing postdoc @sofiquinodoz @brangwynnelab, and awesome collaborators, utilizing genomics, imaging, engineered rDNA🧬, and modeling approaches.

@jiang_lifei @brangwynnelab 💪🤩👩‍🔬🧬🔬 dream team!

@LomvardasLab Thanks so much Stavros!!

Mapping and engineering RNA-controlled architecture of the multiphase nucleolus biorxiv.org/content/10.110… Gorgeous work!

@C_wang_stories Thank you!

@KiWookKim816 Thank you!

@sofiquinodoz WOW! SUPER COOL!

@KalliKappel Thanks so much Kalli! And congrats on your position at UCLA! 🤩

@sofiquinodoz Amazing!! Congrats Sofi!!! 🤩

@daph_cabi Thank you!

Looks super interesting!! Congratulations!

@yodai_takei Thank you Yodai :)!

@sofiquinodoz Congrats, Sofi!

@SuYapeng Thanks so much Yapeng!

@sofiquinodoz Congrats Sofi! Amazing work!

@shrinivaslab Thanks Krishna :) !!

@sofiquinodoz Congratulations @sofiquinodoz :) Look forward to diving in.

Also thanks to @SuperSciJew @DrJorine @danielswlee for initial inspiration/discussions for 5eU imaging to measure rRNA flux, and @mitchguttman @_prashantbhat for initial advice/protocols with 5eU-seq 🙏! Along with many others who helped with this work along the way!!

(12) Together, we find that different rRNA processing steps are not only spatially organized in distinct nucleolar phases, but also play an active role in building and arranging the nucleolar phases that serve as processing compartments.